A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause prob...lem in identification and measurements, hesperidin has been determined successfully.
Keywords:
Direct spectrophotometric method / flavonoids / hesperidin / drug analysis
TY - JOUR
AU - Kuntić, Vesna
AU - Pejić, Nataša
AU - Micić, Svetlana
PY - 2012
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/1734
AB - A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause problem in identification and measurements, hesperidin has been determined successfully.
PB - Slovensko Kemijsko Drustvo, Ljubljana
T2 - Acta Chimica Slovenica
T1 - Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations
VL - 59
IS - 2
SP - 436
EP - 441
ER -
@article{
author = "Kuntić, Vesna and Pejić, Nataša and Micić, Svetlana",
year = "2012",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/1734",
abstract = "A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause problem in identification and measurements, hesperidin has been determined successfully.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations",
volume = "59",
number = "2",
pages = "436-441"
}
Kuntić, V., Pejić, N.,& Micić, S. (2012). Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations.
Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 59(2), 436-441.
Kuntić V, Pejić N, Micić S. Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations. Acta Chimica Slovenica. 2012;59(2):436-441
