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Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations

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2012
1732.pdf (123.1Kb)
Authors
Kuntić, Vesna
Pejić, Nataša
Micić, Svetlana
Article (Published version)
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Abstract
A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause prob...lem in identification and measurements, hesperidin has been determined successfully.

Keywords:
Direct spectrophotometric method / flavonoids / hesperidin / drug analysis
Source:
Acta Chimica Slovenica, 2012, 59, 2, 436-441
Publisher:
  • Slovensko Kemijsko Drustvo, Ljubljana
Projects:
  • Development of molecules with antiinflammatory and cardioprotective activity: structural modifications, modelling, physicochemical characterization and formulation investigations (RS-172041)

ISSN: 1318-0207

PubMed: 24061264

WoS: 000305826700031

Scopus: 2-s2.0-84862848544
[ Google Scholar ]
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/1734
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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