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dc.creatorKuntić, Vesna
dc.creatorPejić, Nataša
dc.creatorMicić, Svetlana
dc.date.accessioned2019-09-02T11:29:46Z
dc.date.available2019-09-02T11:29:46Z
dc.date.issued2012
dc.identifier.issn1318-0207
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1734
dc.description.abstractA simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause problem in identification and measurements, hesperidin has been determined successfully.en
dc.publisherSlovensko Kemijsko Drustvo, Ljubljana
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172041/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceActa Chimica Slovenica
dc.subjectDirect spectrophotometric methoden
dc.subjectflavonoidsen
dc.subjecthesperidinen
dc.subjectdrug analysisen
dc.titleDirect Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparationsen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractМицић, Светлана; Пејић, Наташа; Кунтић, Весна;
dc.citation.volume59
dc.citation.issue2
dc.citation.spage436
dc.citation.epage441
dc.citation.other59(2): 436-441
dc.citation.rankM22
dc.identifier.wos000305826700031
dc.identifier.pmid24061264
dc.identifier.scopus2-s2.0-84862848544
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/549/1732.pdf
dc.identifier.rcubconv_2657
dc.type.versionpublishedVersion


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