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dc.creatorMalenović, Anđelija
dc.creatorJovanović, Marko
dc.creatorPetrović, Sonja
dc.creatorKostić, Nada
dc.creatorVemić, Ana
dc.creatorJančić-Stojanović, Biljana
dc.date.accessioned2019-09-02T11:30:08Z
dc.date.available2019-09-02T11:30:08Z
dc.date.issued2012
dc.identifier.issn1073-9149
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1749
dc.description.abstractIn this article, the multiobjective optimization of reversed-phase high-performance liquid chromatography (RP-HPLC) method for the analysis of folic acid and its two impurities, p-aminobenzoic acid and N-(4-aminobenzoyl)-L-glutamic acid, is presented. During the preliminary study, the independent variables whose impact should be further examined in the optimization were defined (acetonitrile content in the mobile phase, molarity of sodium 1-heptanesulfonate in the aqueous phase, and pH of the aqueous phase). The face-centered central composite design was chosen for the method optimization. As the dependent variables, the separation between the impurities and the retention factor of folic acid were followed. The analysis of variance (ANOVA) test was done, and good correlation among results was confirmed. For the more accurate and reliable optimization, Derringer's desirability function was applied. On the basis of restrictions adopted for the selected targets, the resulting optimal composition of the mobile phase was acetonitrile -3 mM sodium 1-heptanesulfonate (5.3:94.7 V/V), pH of the aqueous phase adjusted to 2.1 with the concentrated ortho-phosphoric acid. Furthermore, the method was subjected to method validation. It proved to be reliable and suitable for the routine analysis of investigated substances in active pharmaceutical ingredients, as well as in pharmaceutical preparations.en
dc.publisherTaylor & Francis Inc, Philadelphia
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceInstrumentation Science & Technology
dc.subjectp-aminobenzoic aciden
dc.subjectN-(4-aminobenzoyl)-L-glutamic aciden
dc.subjectcentral composite designen
dc.subjectdesirability-based optimizationen
dc.subjectfolic aciden
dc.titleOptimization of liquid chromatographic method for the separation of folic acid and its two impuritiesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈанчић-Стојановић, Биљана; Јовановић, Марко; Маленовић, Aнђелија; Петровић, Соња; Костић, Нада; Вемић, Aна;
dc.citation.volume40
dc.citation.issue2-3
dc.citation.spage138
dc.citation.epage149
dc.citation.other40(2-3): 138-149
dc.citation.rankM23
dc.identifier.wos000303580500006
dc.identifier.doi10.1080/10739149.2011.651670
dc.identifier.scopus2-s2.0-84862105705
dc.identifier.rcubconv_2631
dc.type.versionpublishedVersion


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