Приказ основних података о документу
The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum
Efekat predtretmana sa l-name na glutation i glutation peroksidazu u strijatumu pacova na neurotoksičnost izazvanu parakvatom
dc.creator | Đukić, Mirjana | |
dc.creator | Ninković, Milica | |
dc.creator | Stevanović, Ivana | |
dc.creator | Ilić, Katarina | |
dc.creator | Đurđević, Dragan | |
dc.date.accessioned | 2019-09-02T11:30:49Z | |
dc.date.available | 2019-09-02T11:30:49Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0004-1963 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/1778 | |
dc.description.abstract | Contact herbicide paraquat (DQ) is bipyridylium compound, which undergoes redox metabolism; hence enlarged in humans radical production mediated its toxicity. Target organs of systemic effect of PQ poisoning are lung and kidney. The mechanism of PQ-induced neurotoxicity is not elucidated till now. The objective of our study was to examine the role of nitric oxide (NOx) in PQ-induced neurotoxicity, primarily focusing on glutathione cycles [total glutathione content (GSH) and glutathione peroxidase (GPx) activity]. In order to investigate the role of NOx in oxidative stress (OS) and/or nitrosative stress (NS) response to PQ neurotoxicity, we used NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of PQ administration. Study was conducted on Wistar rats randomly divided in groups (n=8 for controls and n=24 for experimental groups) depending on the applied treatments. The tested compounds were intrastriatally (i.s.) administered. Measuring of GSH content and GPx activity was performed at 30 min, 24 hours and 7 days after treatments. Parkinsonism’s like symptoms were observed only in the group of rats treated with PQ. The L-NAME protected animals from PQ-induced neurotoxicity, which could be concluded from the absence of Parkinsonism’s like symptoms and reduced OS/NS response in the striatum of rats pretreated with L-NAME. | en |
dc.description.abstract | Kontaktni herbicid parakvat (PK) je dipiridinsko jedinjenje, koje podleže redoks metabolizmu i svoju toksičnost ispoljava posredstvom povećanog stvaranja slobodnih radikala. Ciljni organi sistemskog toksičnog efekta PK kod čoveka su pluća i bubrezi. Mehanizam PKindukovane neurotoksičnosti do sada još uvek nije u potpunosti rasvetljen. Cilj našeg rada bio je da se ispita uloga azot oksida (NOx) u neurotoksičnosti PK, sa posebnim osvrtom na glutationski ciklus [glutation (GSH) i enzim glutation peroksidazu (GPx)]. U cilju da se istraži uloga NOx u oksidativnom stresu (OS) i / ili nitrosativnom stresu (NS), kao odgovoru na neurotoksičnost PK. U predtretmanu parakvatom koristili smo NG-nitro- L-arginin metil estar (L-NAME), neselektivni inhibitor azot oksid sintetaze (NOS), aplikovan je pre davanja PQ . Studija je sprovedena na pacovima Wistar soja nasumice podeljenim u grupe (n = 8 za kontrolnu grupu i n = 24 za eksperimentalne grupe) u zavisnosti od tretmana. Testirana jedinjenja su intrastrijatalno (i.s.) aplikovana. Merenje sadržaja GSH i aktivnosti GPx izvršena su 30 min, 24 sata i 7 dana posle tretmana. Parkinsonizam je kao simptom primećen samo u grupi pacova tretiranih PK - om. L-NAME je ispoljio zaštitni efekat kod životinja kod kojih je i.s. davan PK, što bi se moglo zaključiti na osnovu odsustva simptoma parkinsonizma i smanjenog OS/NS odgovora u strijatumu u grupi pretretiranoj sa L-NAME. | sr |
dc.publisher | Savez farmaceutskih udruženja Srbije, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41018/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-sa/4.0/ | |
dc.source | Arhiv za farmaciju | |
dc.subject | paraquat | en |
dc.subject | neurotoxicity | en |
dc.subject | striatum | en |
dc.subject | nitric oxide | en |
dc.subject | L-NAME | en |
dc.subject | parakvat | sr |
dc.subject | neurotoksičnost | sr |
dc.subject | strijatum | sr |
dc.subject | azot oksid | sr |
dc.subject | L-NAME | sr |
dc.title | The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum | en |
dc.title | Efekat predtretmana sa l-name na glutation i glutation peroksidazu u strijatumu pacova na neurotoksičnost izazvanu parakvatom | sr |
dc.type | article | |
dc.rights.license | BY-SA | |
dcterms.abstract | Стевановић, Ивана; Ђукић, Мирјана; Нинковић, Милица; Ђурђевић, Драган; Илић, Катарина; Ефекат предтретмана са л-наме на глутатион и глутатион пероксидазу у стријатуму пацова на неуротоксичност изазвану паракватом; Ефекат предтретмана са л-наме на глутатион и глутатион пероксидазу у стријатуму пацова на неуротоксичност изазвану паракватом; | |
dc.citation.volume | 62 | |
dc.citation.issue | 3 | |
dc.citation.spage | 237 | |
dc.citation.epage | 251 | |
dc.citation.other | 62(3): 237-251 | |
dc.citation.rank | M53 | |
dc.identifier.scopus | 2-s2.0-84863806823 | |
dc.identifier.fulltext | https://farfar.pharmacy.bg.ac.rs//bitstream/id/589/1776.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_farfar_1778 | |
dc.type.version | publishedVersion |