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dc.creatorŽunić, G.D
dc.creatorSpasić, S
dc.creatorJelić-Ivanović, Zorana
dc.date.accessioned2019-09-02T11:31:21Z
dc.date.available2019-09-02T11:31:21Z
dc.date.issued2012
dc.identifier.issn1064-3745
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1799
dc.description.abstractWhole blood and/or plasma amino acids are useful for monitoring whole body protein and amino acid metabolism in an organism under various physiological and pathophysiological conditions. Various methodological procedures are in use for their measurement in biological fluids. From the time when capillary electrophoresis was introduced as a technology offering rapid separation of various ionic and/or ionizable compounds with low sample and solvent consumption, there were many attempts to use it for the measurement of amino acids present in physiological fluids. As a rule, these methods require derivatization procedures for detection purposes. Here, we present two protocols for the analysis of free amino acids employing free zone capillary electrophoresis. Main advantage of both methods is an absence of any derivatization procedures that permits the analysis of free amino acid in physiological fluids. The method using direct detection and carrier electrolyte consisting of disodium monophosphate (10 mM at pH 2.90) permits determination of compounds that absorb in UV region (aromatic and sulfur containing amino acids, as well as some peptides, such as carnosine, reduced and oxidized glutathione). The other method uses indirect absorbance detection, employing 8 mM p-amino salicylic acid buffered with sodium carbonate at pH 10.2 as running electrolyte. It permits quantification of 30 underivatized physiological amino acids and peptides. In our experience, factorial design represents a useful tool for final optimization of the electrophoretic conditions if it is necessary.en
dc.rightsrestrictedAccess
dc.sourceMethods in Molecular Biology
dc.subjectAmino acid analysisen
dc.subjectAmino acidsen
dc.subjectBlooden
dc.subjectCapillary electrophoresisen
dc.subjectPlasmaen
dc.titleCapillary electrophoresis of free amino acids in physiological fluids without derivatization employing direct or indirect absorbance detectionen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЖунић, Г.Д; Јелић-Ивановић, Зорана; Спасић, С;
dc.citation.volume828
dc.citation.spage243
dc.citation.epage254
dc.citation.other828: 243-254
dc.identifier.doi10.1007/978-1-61779-445-2_19
dc.identifier.scopus2-s2.0-84555187378
dc.identifier.rcubconv_4905
dc.type.versionpublishedVersion


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