Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms
Razvoj i validacija fluorimetrijske metode za određivanje hesperidina u humanom serumu i farmaceutskim preparatima

2012
Authors
Pavun, Leposava
Dimitrić-Marković, Jasmina
Đurđević, Predrag

Jelikić-Stankov, Milena
Đikanović, Daniela
Ćirić, Andrija

Malešev, Dušan
Article (Published version)
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A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 μg mL-1 with a limit of detection, LOD, of 0.016 μg mL-1 and a limit of quantification, LOQ, of 0.049 μg mL-1. Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 μg mL-1. The LOD was 0.032 μg mL-1 while LOQ was 0.096 μg mL-1. Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC-MS/MS method for plasma samples and an HPLC/UV method for tablets wi...th direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 μg mL-1. The LOD was 0.01 μg mL-1 and the LOQ was 0.03 μg mL-1. The linearity range for the determination of hesperidin in plasma was 0.02-10.00 μg mL-1 with an LOD 0.005 μg mL-1 and an LOQ of 0.015 μg mL-1. The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories.
Razvijena je fluorimetrijska metoda za određivanje hesperidina u humanom serumu i farmaceutskim preparatima koja se zasniva na fluorescenciji kompleksa aluminijum(III)-hesperidin. Kompleks pokazuje intenzivnu fluorescenciju u prisustvu surfaktanta SB 12 na 476 nm prilikom ekscitacije na 390 nm. Linearna zavisnost intenziteta fluorescencije od koncentracije pri određivanju hesperidina u farmaceutskim preparatima dobijena je u koncentracionom opsegu 0,06-24,4 μg mL-1 sa granicom detekcije od 0,016 μg mL-1 i granicom kvantifikacije od 0,049 μg mL-1. Dobijene 'recovery' vrednosti u intervalu 99,3-99,7 % pokazuju veliku preciznost metode. Linearna zavisnost intenziteta fluorescencije kompeksa od koncentacije hesperidina dobijena je u koncentracionom opsegu 0,1-12,2 μg mL-1 sa granicom detekcije od 0,032 μg mL-1 i granicom kvantifikacije od 0,096 μg mL-1. 'Recovery' vrednosti su dobijene u opsegu 98,4 do 99,8 %. Pouzdanost metode proverena je LC-MS/MS metodom za određivanje hesperidina u ser...umu, a HPLC/UV metodom proverena je pouzdanost prilikom određivanja hesperidina u farmaceutskim preparatima. Linearna zavisnost pri određivanju hesperidina u farmaceutskim preparatima dobijena je u intervalu 0,05-10,00 μg mL-1. Granica detekcije je iznosila 0,01, a granica kvantifikacije je 0,03 μg mL-1. Linearna zavisnost pri određivanju hesperidina u humanom serumu je dobijena u intervalu 0,02-10,00 μg mL-1 sa granicom detekcije od 0,005 i granicom kvantifikacije od 0,015 μg mL-1. Dobro slaganje između ove dve metode pokazuje primenljivost fluorimetrijske metode u kliničkim laboratorijama i laboratorijama za kontrolu kvaliteta. Predložena fluorimetrijska metoda je jednostavna, pouzdana i precizna za određivanje hesperidina u humanom serumu i farmaceutskim preparatima.
Keywords:
hesperidin / human plasma / tablets / fluorometry / LC-MS/MSSource:
Journal of the Serbian Chemical Society, 2012, 77, 11, 1625-1640Publisher:
- Srpsko hemijsko društvo, Beograd
Funding / projects:
- Synthesis, modeling, physicochemical and biological properties of organic compounds and related metal complexes (RS-172016)
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)
DOI: 10.2298/JSC111005060P
ISSN: 0352-5139
WoS: 000312746700010
Scopus: 2-s2.0-84871823513
Collections
Institution/Community
PharmacyTY - JOUR AU - Pavun, Leposava AU - Dimitrić-Marković, Jasmina AU - Đurđević, Predrag AU - Jelikić-Stankov, Milena AU - Đikanović, Daniela AU - Ćirić, Andrija AU - Malešev, Dušan PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1821 AB - A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 μg mL-1 with a limit of detection, LOD, of 0.016 μg mL-1 and a limit of quantification, LOQ, of 0.049 μg mL-1. Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 μg mL-1. The LOD was 0.032 μg mL-1 while LOQ was 0.096 μg mL-1. Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC-MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 μg mL-1. The LOD was 0.01 μg mL-1 and the LOQ was 0.03 μg mL-1. The linearity range for the determination of hesperidin in plasma was 0.02-10.00 μg mL-1 with an LOD 0.005 μg mL-1 and an LOQ of 0.015 μg mL-1. The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories. AB - Razvijena je fluorimetrijska metoda za određivanje hesperidina u humanom serumu i farmaceutskim preparatima koja se zasniva na fluorescenciji kompleksa aluminijum(III)-hesperidin. Kompleks pokazuje intenzivnu fluorescenciju u prisustvu surfaktanta SB 12 na 476 nm prilikom ekscitacije na 390 nm. Linearna zavisnost intenziteta fluorescencije od koncentracije pri određivanju hesperidina u farmaceutskim preparatima dobijena je u koncentracionom opsegu 0,06-24,4 μg mL-1 sa granicom detekcije od 0,016 μg mL-1 i granicom kvantifikacije od 0,049 μg mL-1. Dobijene 'recovery' vrednosti u intervalu 99,3-99,7 % pokazuju veliku preciznost metode. Linearna zavisnost intenziteta fluorescencije kompeksa od koncentacije hesperidina dobijena je u koncentracionom opsegu 0,1-12,2 μg mL-1 sa granicom detekcije od 0,032 μg mL-1 i granicom kvantifikacije od 0,096 μg mL-1. 'Recovery' vrednosti su dobijene u opsegu 98,4 do 99,8 %. Pouzdanost metode proverena je LC-MS/MS metodom za određivanje hesperidina u serumu, a HPLC/UV metodom proverena je pouzdanost prilikom određivanja hesperidina u farmaceutskim preparatima. Linearna zavisnost pri određivanju hesperidina u farmaceutskim preparatima dobijena je u intervalu 0,05-10,00 μg mL-1. Granica detekcije je iznosila 0,01, a granica kvantifikacije je 0,03 μg mL-1. Linearna zavisnost pri određivanju hesperidina u humanom serumu je dobijena u intervalu 0,02-10,00 μg mL-1 sa granicom detekcije od 0,005 i granicom kvantifikacije od 0,015 μg mL-1. Dobro slaganje između ove dve metode pokazuje primenljivost fluorimetrijske metode u kliničkim laboratorijama i laboratorijama za kontrolu kvaliteta. Predložena fluorimetrijska metoda je jednostavna, pouzdana i precizna za određivanje hesperidina u humanom serumu i farmaceutskim preparatima. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms T1 - Razvoj i validacija fluorimetrijske metode za određivanje hesperidina u humanom serumu i farmaceutskim preparatima VL - 77 IS - 11 SP - 1625 EP - 1640 DO - 10.2298/JSC111005060P ER -
@article{ author = "Pavun, Leposava and Dimitrić-Marković, Jasmina and Đurđević, Predrag and Jelikić-Stankov, Milena and Đikanović, Daniela and Ćirić, Andrija and Malešev, Dušan", year = "2012", abstract = "A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 μg mL-1 with a limit of detection, LOD, of 0.016 μg mL-1 and a limit of quantification, LOQ, of 0.049 μg mL-1. Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 μg mL-1. The LOD was 0.032 μg mL-1 while LOQ was 0.096 μg mL-1. Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC-MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 μg mL-1. The LOD was 0.01 μg mL-1 and the LOQ was 0.03 μg mL-1. The linearity range for the determination of hesperidin in plasma was 0.02-10.00 μg mL-1 with an LOD 0.005 μg mL-1 and an LOQ of 0.015 μg mL-1. The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories., Razvijena je fluorimetrijska metoda za određivanje hesperidina u humanom serumu i farmaceutskim preparatima koja se zasniva na fluorescenciji kompleksa aluminijum(III)-hesperidin. Kompleks pokazuje intenzivnu fluorescenciju u prisustvu surfaktanta SB 12 na 476 nm prilikom ekscitacije na 390 nm. Linearna zavisnost intenziteta fluorescencije od koncentracije pri određivanju hesperidina u farmaceutskim preparatima dobijena je u koncentracionom opsegu 0,06-24,4 μg mL-1 sa granicom detekcije od 0,016 μg mL-1 i granicom kvantifikacije od 0,049 μg mL-1. Dobijene 'recovery' vrednosti u intervalu 99,3-99,7 % pokazuju veliku preciznost metode. Linearna zavisnost intenziteta fluorescencije kompeksa od koncentacije hesperidina dobijena je u koncentracionom opsegu 0,1-12,2 μg mL-1 sa granicom detekcije od 0,032 μg mL-1 i granicom kvantifikacije od 0,096 μg mL-1. 'Recovery' vrednosti su dobijene u opsegu 98,4 do 99,8 %. Pouzdanost metode proverena je LC-MS/MS metodom za određivanje hesperidina u serumu, a HPLC/UV metodom proverena je pouzdanost prilikom određivanja hesperidina u farmaceutskim preparatima. Linearna zavisnost pri određivanju hesperidina u farmaceutskim preparatima dobijena je u intervalu 0,05-10,00 μg mL-1. Granica detekcije je iznosila 0,01, a granica kvantifikacije je 0,03 μg mL-1. Linearna zavisnost pri određivanju hesperidina u humanom serumu je dobijena u intervalu 0,02-10,00 μg mL-1 sa granicom detekcije od 0,005 i granicom kvantifikacije od 0,015 μg mL-1. Dobro slaganje između ove dve metode pokazuje primenljivost fluorimetrijske metode u kliničkim laboratorijama i laboratorijama za kontrolu kvaliteta. Predložena fluorimetrijska metoda je jednostavna, pouzdana i precizna za određivanje hesperidina u humanom serumu i farmaceutskim preparatima.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms, Razvoj i validacija fluorimetrijske metode za određivanje hesperidina u humanom serumu i farmaceutskim preparatima", volume = "77", number = "11", pages = "1625-1640", doi = "10.2298/JSC111005060P" }
Pavun, L., Dimitrić-Marković, J., Đurđević, P., Jelikić-Stankov, M., Đikanović, D., Ćirić, A.,& Malešev, D.. (2012). Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 77(11), 1625-1640. https://doi.org/10.2298/JSC111005060P
Pavun L, Dimitrić-Marković J, Đurđević P, Jelikić-Stankov M, Đikanović D, Ćirić A, Malešev D. Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms. in Journal of the Serbian Chemical Society. 2012;77(11):1625-1640. doi:10.2298/JSC111005060P .
Pavun, Leposava, Dimitrić-Marković, Jasmina, Đurđević, Predrag, Jelikić-Stankov, Milena, Đikanović, Daniela, Ćirić, Andrija, Malešev, Dušan, "Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms" in Journal of the Serbian Chemical Society, 77, no. 11 (2012):1625-1640, https://doi.org/10.2298/JSC111005060P . .