Fluorometric determination of hesperidin in orange juices available on Serbian market
Fluorometrijsko određivanje hesperidina u sokovima od pomorandže prisutnim na tržištu Srbije

2012
Authors
Pavun, Leposava
Jelikić-Stankov, Milena
Malešev, Dušan
Uskoković-Marković, Snežana

Dimitrić-Marković, Jasmina
Đurđević, Predrag

Đikanović, Daniela
Article (Published version)
Metadata
Show full item recordAbstract
The spectrofluorometric method based on fluorescence ability of aluminium - hesperidin complex for the determination of hesperidin in orange juice is proposed. The linearity range of hesperidin in methanolicaqueous solution was 0.08 - 18.0 μg/mL with LOD and LOQ values as 0.023 μg/mL and 0.070 μg/mL, respectively, and recovery values in the range 97.8 - 99.7 %. Method was simplified by omitting any of surphactant agents usually applied in similar procedures, and successfully applied for the determination of hesperidin in orange juices commercially available on the Serbian market.
U radu je predložena spektrofluorimetrijska metoda za određivanje sadržaja hesperidina u sokovima od pomorandže zasnovana na sposobnosti fluorescencije kompleksa aluminijum - hesperidin. Utvrđena je oblast linearnosti za određivanje hesperidina u metanolno-vodenim rastvora od 0.08 do 18.0 μg/mL, pri čemu je LOD= 0.023 μg/mL i LOQ= 0.070 μg/mL, a recovery vrednost 97.8 - 99.7 %. Metoda je pojednostavljena izostavljanjem često korišćenih površinski aktivnih materija koje se koriste u sličnim procedurama i uspešno je primenjena za određivanje sadržaja hesperidina u sokovima od pomorandže prisutnim na tržištu Srbije.
Keywords:
hesperidin / orange juice / spectrofluorometry / hesperidin / sok od pomorandže / spektrofluorometrijaSource:
Acta agriculturae Serbica, 2012, 17, 34, 93-103Publisher:
- Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
Projects:
- Synthesis, modeling, physicochemical and biological properties of organic compounds and related metal complexes (RS-172016)
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)
- Electroconducting and redox-active polymers and oligomers: synthesis, structure, properties and applications (RS-172043)