The spectrofluorometric method based on fluorescence ability of aluminium - hesperidin complex for the determination of hesperidin in orange juice is proposed. The linearity range of hesperidin in methanolicaqueous solution was 0.08 - 18.0 μg/mL with LOD and LOQ values as 0.023 μg/mL and 0.070 μg/mL, respectively, and recovery values in the range 97.8 - 99.7 %. Method was simplified by omitting any of surphactant agents usually applied in similar procedures, and successfully applied for the determination of hesperidin in orange juices commercially available on the Serbian market.
U radu je predložena spektrofluorimetrijska metoda za određivanje sadržaja hesperidina u sokovima od pomorandže zasnovana na sposobnosti fluorescencije kompleksa aluminijum - hesperidin. Utvrđena je oblast linearnosti za određivanje hesperidina u metanolno-vodenim rastvora od 0.08 do 18.0 μg/mL, pri čemu je LOD= 0.023 μg/mL i LOQ= 0.070 μg/mL, a recovery vrednost 97.8 - 99.7 %. Metoda je pojednostavljena izostavljanjem često korišćenih površinski aktivnih materija koje se koriste u sličnim procedurama i uspešno je primenjena za određivanje sadržaja hesperidina u sokovima od pomorandže prisutnim na tržištu Srbije.
Keywords:
hesperidin / orange juice / spectrofluorometry / hesperidin / sok od pomorandže / spektrofluorometrija
Source:
Acta agriculturae Serbica, 2012, 17, 34, 93-103
Publisher:
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
TY - JOUR
AU - Pavun, Leposava
AU - Jelikić-Stankov, Milena
AU - Malešev, Dušan
AU - Uskoković-Marković, Snežana
AU - Dimitrić-Marković, Jasmina
AU - Đurđević, Predrag
AU - Đikanović, Daniela
PY - 2012
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1824
AB - The spectrofluorometric method based on fluorescence ability of aluminium - hesperidin complex for the determination of hesperidin in orange juice is proposed. The linearity range of hesperidin in methanolicaqueous solution was 0.08 - 18.0 μg/mL with LOD and LOQ values as 0.023 μg/mL and 0.070 μg/mL, respectively, and recovery values in the range 97.8 - 99.7 %. Method was simplified by omitting any of surphactant agents usually applied in similar procedures, and successfully applied for the determination of hesperidin in orange juices commercially available on the Serbian market.
AB - U radu je predložena spektrofluorimetrijska metoda za određivanje sadržaja hesperidina u sokovima od pomorandže zasnovana na sposobnosti fluorescencije kompleksa aluminijum - hesperidin. Utvrđena je oblast linearnosti za određivanje hesperidina u metanolno-vodenim rastvora od 0.08 do 18.0 μg/mL, pri čemu je LOD= 0.023 μg/mL i LOQ= 0.070 μg/mL, a recovery vrednost 97.8 - 99.7 %. Metoda je pojednostavljena izostavljanjem često korišćenih površinski aktivnih materija koje se koriste u sličnim procedurama i uspešno je primenjena za određivanje sadržaja hesperidina u sokovima od pomorandže prisutnim na tržištu Srbije.
PB - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2 - Acta agriculturae Serbica
T1 - Fluorometric determination of hesperidin in orange juices available on Serbian market
T1 - Fluorometrijsko određivanje hesperidina u sokovima od pomorandže prisutnim na tržištu Srbije
VL - 17
IS - 34
SP - 93
EP - 103
UR - https://hdl.handle.net/21.15107/rcub_farfar_1824
ER -
@article{
author = "Pavun, Leposava and Jelikić-Stankov, Milena and Malešev, Dušan and Uskoković-Marković, Snežana and Dimitrić-Marković, Jasmina and Đurđević, Predrag and Đikanović, Daniela",
year = "2012",
abstract = "The spectrofluorometric method based on fluorescence ability of aluminium - hesperidin complex for the determination of hesperidin in orange juice is proposed. The linearity range of hesperidin in methanolicaqueous solution was 0.08 - 18.0 μg/mL with LOD and LOQ values as 0.023 μg/mL and 0.070 μg/mL, respectively, and recovery values in the range 97.8 - 99.7 %. Method was simplified by omitting any of surphactant agents usually applied in similar procedures, and successfully applied for the determination of hesperidin in orange juices commercially available on the Serbian market., U radu je predložena spektrofluorimetrijska metoda za određivanje sadržaja hesperidina u sokovima od pomorandže zasnovana na sposobnosti fluorescencije kompleksa aluminijum - hesperidin. Utvrđena je oblast linearnosti za određivanje hesperidina u metanolno-vodenim rastvora od 0.08 do 18.0 μg/mL, pri čemu je LOD= 0.023 μg/mL i LOQ= 0.070 μg/mL, a recovery vrednost 97.8 - 99.7 %. Metoda je pojednostavljena izostavljanjem često korišćenih površinski aktivnih materija koje se koriste u sličnim procedurama i uspešno je primenjena za određivanje sadržaja hesperidina u sokovima od pomorandže prisutnim na tržištu Srbije.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Fluorometric determination of hesperidin in orange juices available on Serbian market, Fluorometrijsko određivanje hesperidina u sokovima od pomorandže prisutnim na tržištu Srbije",
volume = "17",
number = "34",
pages = "93-103",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1824"
}
Pavun, L., Jelikić-Stankov, M., Malešev, D., Uskoković-Marković, S., Dimitrić-Marković, J., Đurđević, P.,& Đikanović, D.. (2012). Fluorometric determination of hesperidin in orange juices available on Serbian market. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 17(34), 93-103.
https://hdl.handle.net/21.15107/rcub_farfar_1824
Pavun L, Jelikić-Stankov M, Malešev D, Uskoković-Marković S, Dimitrić-Marković J, Đurđević P, Đikanović D. Fluorometric determination of hesperidin in orange juices available on Serbian market. in Acta agriculturae Serbica. 2012;17(34):93-103.
https://hdl.handle.net/21.15107/rcub_farfar_1824 .
Pavun, Leposava, Jelikić-Stankov, Milena, Malešev, Dušan, Uskoković-Marković, Snežana, Dimitrić-Marković, Jasmina, Đurđević, Predrag, Đikanović, Daniela, "Fluorometric determination of hesperidin in orange juices available on Serbian market" in Acta agriculturae Serbica, 17, no. 34 (2012):93-103,
https://hdl.handle.net/21.15107/rcub_farfar_1824 .
