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dc.creatorDimitrijević, Mirjana
dc.creatorStanojević, Stanislava
dc.creatorKustrimović, Nataša
dc.creatorMitić, Katarina
dc.creatorVujić, Vesna
dc.creatorAleksić, Iva
dc.creatorRadojević, Katarina
dc.creatorLeposavić, Gordana
dc.date.accessioned2019-09-02T11:32:20Z
dc.date.available2019-09-02T11:32:20Z
dc.date.issued2013
dc.identifier.issn0531-5565
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1840
dc.description.abstractThe phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy;en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//
dc.rightsrestrictedAccess
dc.sourceExperimental Gerontology
dc.subjectAgingen
dc.subjectEstradiol/progesterone balanceen
dc.subjectMacrophagesen
dc.subjectTNF-alphaen
dc.subjectNOen
dc.subjectRaten
dc.titleThe influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha productionen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРадојевић, Катарина; Станојевић, Станислава; Митић, Катарина; Вујић, Весна; Aлексић, Ива; Лепосавић, Гордана; Кустримовић, Наташа; Димитријевић, Мирјана;
dc.citation.volume48
dc.citation.issue11
dc.citation.spage1243
dc.citation.epage1254
dc.citation.other48(11): 1243-1254
dc.citation.rankM21
dc.identifier.wos000325750900014
dc.identifier.doi10.1016/j.exger.2013.07.001
dc.identifier.pmid23850866
dc.identifier.scopus2-s2.0-84883649839
dc.identifier.rcubconv_2934
dc.type.versionpublishedVersion


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