Raman spectroscopy enables non-invasive investigation of chemical composition of biological tissues. Due to similar chemical composition, the analysis of Raman spectra of brain structures and assignment of their spectral features to chemical constituents presents a particular challenge. In this study we demonstrate that standard and independent component analysis of Raman spectra is capable of assessment of differences in chemical composition between functionally related gray and white matter structures. Our results show the ability of Raman spectroscopy to successfully depict variation in chemical composition between structurally similar and/or functionally connected brain structures. The observed differences were attributed to variations in content of proteins and lipids in these structures. Independent component analysis enabled separation of contributions of major constituents in spectra and revealed spectral signatures of low-concentration metabolites. This provided finding of dis...crepancies between structures of striatum as well as between white matter structures. Raman spectroscopy can provide information about variations in contents of major chemical constituents in brain structures, while the application of independent component analysis performed on obtained spectra can help in revealing minute differences between closely related brain structures.
TY - JOUR
AU - Daković, Marko
AU - Stojiljković, Aleksandra S.
AU - Bajuk-Bogdanović, Danica
AU - Starcević, Ana
AU - Puskas, Laslo
AU - Filipović, Branislav
AU - Uskoković-Marković, Snežana
AU - Holclajtner-Antunović, Ivanka
PY - 2013
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/1883
AB - Raman spectroscopy enables non-invasive investigation of chemical composition of biological tissues. Due to similar chemical composition, the analysis of Raman spectra of brain structures and assignment of their spectral features to chemical constituents presents a particular challenge. In this study we demonstrate that standard and independent component analysis of Raman spectra is capable of assessment of differences in chemical composition between functionally related gray and white matter structures. Our results show the ability of Raman spectroscopy to successfully depict variation in chemical composition between structurally similar and/or functionally connected brain structures. The observed differences were attributed to variations in content of proteins and lipids in these structures. Independent component analysis enabled separation of contributions of major constituents in spectra and revealed spectral signatures of low-concentration metabolites. This provided finding of discrepancies between structures of striatum as well as between white matter structures. Raman spectroscopy can provide information about variations in contents of major chemical constituents in brain structures, while the application of independent component analysis performed on obtained spectra can help in revealing minute differences between closely related brain structures.
PB - Elsevier Science BV, Amsterdam
T2 - Talanta
T1 - Profiling differences in chemical composition of brain structures using Raman spectroscopy
VL - 117
SP - 133
EP - 138
DO - 10.1016/j.talanta.2013.08.058
ER -
@article{
author = "Daković, Marko and Stojiljković, Aleksandra S. and Bajuk-Bogdanović, Danica and Starcević, Ana and Puskas, Laslo and Filipović, Branislav and Uskoković-Marković, Snežana and Holclajtner-Antunović, Ivanka",
year = "2013",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/1883",
abstract = "Raman spectroscopy enables non-invasive investigation of chemical composition of biological tissues. Due to similar chemical composition, the analysis of Raman spectra of brain structures and assignment of their spectral features to chemical constituents presents a particular challenge. In this study we demonstrate that standard and independent component analysis of Raman spectra is capable of assessment of differences in chemical composition between functionally related gray and white matter structures. Our results show the ability of Raman spectroscopy to successfully depict variation in chemical composition between structurally similar and/or functionally connected brain structures. The observed differences were attributed to variations in content of proteins and lipids in these structures. Independent component analysis enabled separation of contributions of major constituents in spectra and revealed spectral signatures of low-concentration metabolites. This provided finding of discrepancies between structures of striatum as well as between white matter structures. Raman spectroscopy can provide information about variations in contents of major chemical constituents in brain structures, while the application of independent component analysis performed on obtained spectra can help in revealing minute differences between closely related brain structures.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Talanta",
title = "Profiling differences in chemical composition of brain structures using Raman spectroscopy",
volume = "117",
pages = "133-138",
doi = "10.1016/j.talanta.2013.08.058"
}
Daković, M., Stojiljković, A. S., Bajuk-Bogdanović, D., Starcević, A., Puskas, L., Filipović, B., Uskoković-Marković, S.,& Holclajtner-Antunović, I. (2013). Profiling differences in chemical composition of brain structures using Raman spectroscopy.
Talanta
Elsevier Science BV, Amsterdam., 117, 133-138.
https://doi.org/10.1016/j.talanta.2013.08.058
Daković M, Stojiljković AS, Bajuk-Bogdanović D, Starcević A, Puskas L, Filipović B, Uskoković-Marković S, Holclajtner-Antunović I. Profiling differences in chemical composition of brain structures using Raman spectroscopy. Talanta. 2013;117:133-138
Daković Marko, Stojiljković Aleksandra S., Bajuk-Bogdanović Danica, Starcević Ana, Puskas Laslo, Filipović Branislav, Uskoković-Marković Snežana, Holclajtner-Antunović Ivanka, "Profiling differences in chemical composition of brain structures using Raman spectroscopy" Talanta, 117 (2013):133-138,
https://doi.org/10.1016/j.talanta.2013.08.058 .
