Role of ovarian hormones in T-cell homeostasis: From the thymus to the periphery
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2013
Authors
Perišić, MilicaStojić-Vukanić, Zorica

Pilipović, Ivan
Kosec, Duško
Nacka-Aleksić, Mirjana

Đikić, Jasmina

Arsenović-Ranin, Nevena

Leposavić, Gordana

Article (Published version)

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The study explored the putative role of ovarian hormones in the peripubertal remodelling of peripheral T-cell compartment. Ovariectomy at age of 1 month enhanced the peripubertal rise in CD4+ and CD8+ cell numbers in peripheral blood (PB) and spleen from 2-month-old rats. This reflected maintenance of thymopoietic efficiency at the prepubertal level (judging by numbers of the most mature CD4+ and CD8+ thymocytes and recent thymic emigrants) and alterations in T-cell survival/proliferation in the periphery. Compared with age-matched controls, the frequency of apoptotic cells among CD8+ peripheral blood lymphocytes (PBLs) and CD4+ and CD8+ splenocytes was diminished in ovariectomized (Ox) rats, at least partly, due to lower CD95 surface density. The diminished frequency of the apoptotic T splenocytes could also be associated with the rise in the amount of splenic IL-7 mRNA. Additionally, the latter finding was consistent with the augmented proliferation of CD4+ and CD8+ splenocytes. Howe...ver, the enhanced proliferation of these cells could also be linked to the rise in IL-2 receptor surface density. This increase was related to the enhanced splenic TNF-alpha mRNA expression. Additionally, ovariectomy led to the phenotypic alterations in the major PBL and splenic T-cell subsets by diminishing/preventing the peripubertal changes in the frequency of cells at distinct stages of post-thymic differentiation/maturation (recent thymic emigrants, mature naive and memory cells), and by decreasing the frequency of NKT cells within peripheral CD8+ subsets. In addition to numerical and phenotypic changes in T-cell compartment (due to the lack of ovarian hormone action at both the thymic and peripheral level), Ox rats exhibited a much larger delayed-type hypersensitivity (DTH) response compared with age-matched controls. This suggested the augmented T-cell-mediated immune response in Ox rats compared with aged-matched controls.
Keywords:
Peripubertal development / Ovarian hormones / T-cell proliferation / T-cell apoptosis / Peripheral T-cell maturation / Immunoregulatory T-cellsSource:
Immunobiology, 2013, 218, 3, 353-367Publisher:
- Elsevier Gmbh, Urban & Fischer Verlag, Jena
Funding / projects:
DOI: 10.1016/j.imbio.2012.05.009
ISSN: 0171-2985
PubMed: 22704521
WoS: 000316235700008
Scopus: 2-s2.0-84873258455
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PharmacyTY - JOUR AU - Perišić, Milica AU - Stojić-Vukanić, Zorica AU - Pilipović, Ivan AU - Kosec, Duško AU - Nacka-Aleksić, Mirjana AU - Đikić, Jasmina AU - Arsenović-Ranin, Nevena AU - Leposavić, Gordana PY - 2013 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1915 AB - The study explored the putative role of ovarian hormones in the peripubertal remodelling of peripheral T-cell compartment. Ovariectomy at age of 1 month enhanced the peripubertal rise in CD4+ and CD8+ cell numbers in peripheral blood (PB) and spleen from 2-month-old rats. This reflected maintenance of thymopoietic efficiency at the prepubertal level (judging by numbers of the most mature CD4+ and CD8+ thymocytes and recent thymic emigrants) and alterations in T-cell survival/proliferation in the periphery. Compared with age-matched controls, the frequency of apoptotic cells among CD8+ peripheral blood lymphocytes (PBLs) and CD4+ and CD8+ splenocytes was diminished in ovariectomized (Ox) rats, at least partly, due to lower CD95 surface density. The diminished frequency of the apoptotic T splenocytes could also be associated with the rise in the amount of splenic IL-7 mRNA. Additionally, the latter finding was consistent with the augmented proliferation of CD4+ and CD8+ splenocytes. However, the enhanced proliferation of these cells could also be linked to the rise in IL-2 receptor surface density. This increase was related to the enhanced splenic TNF-alpha mRNA expression. Additionally, ovariectomy led to the phenotypic alterations in the major PBL and splenic T-cell subsets by diminishing/preventing the peripubertal changes in the frequency of cells at distinct stages of post-thymic differentiation/maturation (recent thymic emigrants, mature naive and memory cells), and by decreasing the frequency of NKT cells within peripheral CD8+ subsets. In addition to numerical and phenotypic changes in T-cell compartment (due to the lack of ovarian hormone action at both the thymic and peripheral level), Ox rats exhibited a much larger delayed-type hypersensitivity (DTH) response compared with age-matched controls. This suggested the augmented T-cell-mediated immune response in Ox rats compared with aged-matched controls. PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena T2 - Immunobiology T1 - Role of ovarian hormones in T-cell homeostasis: From the thymus to the periphery VL - 218 IS - 3 SP - 353 EP - 367 DO - 10.1016/j.imbio.2012.05.009 ER -
@article{ author = "Perišić, Milica and Stojić-Vukanić, Zorica and Pilipović, Ivan and Kosec, Duško and Nacka-Aleksić, Mirjana and Đikić, Jasmina and Arsenović-Ranin, Nevena and Leposavić, Gordana", year = "2013", abstract = "The study explored the putative role of ovarian hormones in the peripubertal remodelling of peripheral T-cell compartment. Ovariectomy at age of 1 month enhanced the peripubertal rise in CD4+ and CD8+ cell numbers in peripheral blood (PB) and spleen from 2-month-old rats. This reflected maintenance of thymopoietic efficiency at the prepubertal level (judging by numbers of the most mature CD4+ and CD8+ thymocytes and recent thymic emigrants) and alterations in T-cell survival/proliferation in the periphery. Compared with age-matched controls, the frequency of apoptotic cells among CD8+ peripheral blood lymphocytes (PBLs) and CD4+ and CD8+ splenocytes was diminished in ovariectomized (Ox) rats, at least partly, due to lower CD95 surface density. The diminished frequency of the apoptotic T splenocytes could also be associated with the rise in the amount of splenic IL-7 mRNA. Additionally, the latter finding was consistent with the augmented proliferation of CD4+ and CD8+ splenocytes. However, the enhanced proliferation of these cells could also be linked to the rise in IL-2 receptor surface density. This increase was related to the enhanced splenic TNF-alpha mRNA expression. Additionally, ovariectomy led to the phenotypic alterations in the major PBL and splenic T-cell subsets by diminishing/preventing the peripubertal changes in the frequency of cells at distinct stages of post-thymic differentiation/maturation (recent thymic emigrants, mature naive and memory cells), and by decreasing the frequency of NKT cells within peripheral CD8+ subsets. In addition to numerical and phenotypic changes in T-cell compartment (due to the lack of ovarian hormone action at both the thymic and peripheral level), Ox rats exhibited a much larger delayed-type hypersensitivity (DTH) response compared with age-matched controls. This suggested the augmented T-cell-mediated immune response in Ox rats compared with aged-matched controls.", publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena", journal = "Immunobiology", title = "Role of ovarian hormones in T-cell homeostasis: From the thymus to the periphery", volume = "218", number = "3", pages = "353-367", doi = "10.1016/j.imbio.2012.05.009" }
Perišić, M., Stojić-Vukanić, Z., Pilipović, I., Kosec, D., Nacka-Aleksić, M., Đikić, J., Arsenović-Ranin, N.,& Leposavić, G.. (2013). Role of ovarian hormones in T-cell homeostasis: From the thymus to the periphery. in Immunobiology Elsevier Gmbh, Urban & Fischer Verlag, Jena., 218(3), 353-367. https://doi.org/10.1016/j.imbio.2012.05.009
Perišić M, Stojić-Vukanić Z, Pilipović I, Kosec D, Nacka-Aleksić M, Đikić J, Arsenović-Ranin N, Leposavić G. Role of ovarian hormones in T-cell homeostasis: From the thymus to the periphery. in Immunobiology. 2013;218(3):353-367. doi:10.1016/j.imbio.2012.05.009 .
Perišić, Milica, Stojić-Vukanić, Zorica, Pilipović, Ivan, Kosec, Duško, Nacka-Aleksić, Mirjana, Đikić, Jasmina, Arsenović-Ranin, Nevena, Leposavić, Gordana, "Role of ovarian hormones in T-cell homeostasis: From the thymus to the periphery" in Immunobiology, 218, no. 3 (2013):353-367, https://doi.org/10.1016/j.imbio.2012.05.009 . .
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