Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training

2013
Authors
Vujović, AnaSpasojević-Kalimanovska, Vesna

Bogavac-Stanojević, Nataša

Kotur-Stevuljević, Jelena

Sopić, Miron

Stefanović, Aleksandra

Baralić, Ivana
Đorđević, Brižita

Jelić-Ivanović, Zorana

Spasić, Slavica
Article (Published version)
Metadata
Show full item recordAbstract
The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O-2(.-)), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O-2(.-) concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, althoug...h they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of AIDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O-2(.-) and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.
Keywords:
gene expression / Cu/Zn SOD / Mn SOD / soccer training / oxidative stressSource:
Biotechnology & Biotechnological Equipment, 2013, 27, 3, 3843-3847Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
DOI: 10.5504/BBEQ.2012.0144
ISSN: 1310-2818
WoS: 000320828500016
Scopus: 2-s2.0-84883257146
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Institution/Community
PharmacyTY - JOUR AU - Vujović, Ana AU - Spasojević-Kalimanovska, Vesna AU - Bogavac-Stanojević, Nataša AU - Kotur-Stevuljević, Jelena AU - Sopić, Miron AU - Stefanović, Aleksandra AU - Baralić, Ivana AU - Đorđević, Brižita AU - Jelić-Ivanović, Zorana AU - Spasić, Slavica PY - 2013 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1919 AB - The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O-2(.-)), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O-2(.-) concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, although they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of AIDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O-2(.-) and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed. PB - Taylor & Francis Ltd, Abingdon T2 - Biotechnology & Biotechnological Equipment T1 - Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training VL - 27 IS - 3 SP - 3843 EP - 3847 DO - 10.5504/BBEQ.2012.0144 ER -
@article{ author = "Vujović, Ana and Spasojević-Kalimanovska, Vesna and Bogavac-Stanojević, Nataša and Kotur-Stevuljević, Jelena and Sopić, Miron and Stefanović, Aleksandra and Baralić, Ivana and Đorđević, Brižita and Jelić-Ivanović, Zorana and Spasić, Slavica", year = "2013", abstract = "The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O-2(.-)), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O-2(.-) concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, although they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of AIDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O-2(.-) and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Biotechnology & Biotechnological Equipment", title = "Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training", volume = "27", number = "3", pages = "3843-3847", doi = "10.5504/BBEQ.2012.0144" }
Vujović, A., Spasojević-Kalimanovska, V., Bogavac-Stanojević, N., Kotur-Stevuljević, J., Sopić, M., Stefanović, A., Baralić, I., Đorđević, B., Jelić-Ivanović, Z.,& Spasić, S.. (2013). Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training. in Biotechnology & Biotechnological Equipment Taylor & Francis Ltd, Abingdon., 27(3), 3843-3847. https://doi.org/10.5504/BBEQ.2012.0144
Vujović A, Spasojević-Kalimanovska V, Bogavac-Stanojević N, Kotur-Stevuljević J, Sopić M, Stefanović A, Baralić I, Đorđević B, Jelić-Ivanović Z, Spasić S. Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training. in Biotechnology & Biotechnological Equipment. 2013;27(3):3843-3847. doi:10.5504/BBEQ.2012.0144 .
Vujović, Ana, Spasojević-Kalimanovska, Vesna, Bogavac-Stanojević, Nataša, Kotur-Stevuljević, Jelena, Sopić, Miron, Stefanović, Aleksandra, Baralić, Ivana, Đorđević, Brižita, Jelić-Ivanović, Zorana, Spasić, Slavica, "Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training" in Biotechnology & Biotechnological Equipment, 27, no. 3 (2013):3843-3847, https://doi.org/10.5504/BBEQ.2012.0144 . .