FarFaR - Pharmacy Repository
University of Belgrade, Faculty of Pharmacy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrilic)
    • Serbian (Latin)
  • Login
View Item 
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training

Thumbnail
2013
1917.pdf (658.6Kb)
Authors
Vujović, Ana
Spasojević-Kalimanovska, Vesna
Bogavac-Stanojević, Nataša
Kotur-Stevuljević, Jelena
Sopić, Miron
Stefanović, Aleksandra
Baralić, Ivana
Đorđević, Brižita
Jelić-Ivanović, Zorana
Spasić, Slavica
Article (Published version)
Metadata
Show full item record
Abstract
The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O-2(.-)), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O-2(.-) concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, althoug...h they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of AIDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O-2(.-) and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.

Keywords:
gene expression / Cu/Zn SOD / Mn SOD / soccer training / oxidative stress
Source:
Biotechnology & Biotechnological Equipment, 2013, 27, 3, 3843-3847
Publisher:
  • Taylor & Francis Ltd, Abingdon
Projects:
  • Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers (RS-175035)

DOI: 10.5504/BBEQ.2012.0144

ISSN: 1310-2818

WoS: 000320828500016

Scopus: 2-s2.0-84883257146
[ Google Scholar ]
1
1
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/1919
Collections
  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutionsAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB