The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O-2(.-)), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O-2(.-) concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, althoug...h they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of AIDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O-2(.-) and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.
TY - JOUR
AU - Vujović, Ana
AU - Spasojević-Kalimanovska, Vesna
AU - Bogavac-Stanojević, Nataša
AU - Kotur-Stevuljević, Jelena
AU - Sopić, Miron
AU - Stefanović, Aleksandra
AU - Baralić, Ivana
AU - Đorđević, Brižita
AU - Jelić-Ivanović, Zorana
AU - Spasić, Slavica
PY - 2013
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1919
AB - The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O-2(.-)), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O-2(.-) concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, although they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of AIDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O-2(.-) and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.
PB - Taylor & Francis Ltd, Abingdon
T2 - Biotechnology & Biotechnological Equipment
T1 - Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training
VL - 27
IS - 3
SP - 3843
EP - 3847
DO - 10.5504/BBEQ.2012.0144
ER -
@article{
author = "Vujović, Ana and Spasojević-Kalimanovska, Vesna and Bogavac-Stanojević, Nataša and Kotur-Stevuljević, Jelena and Sopić, Miron and Stefanović, Aleksandra and Baralić, Ivana and Đorđević, Brižita and Jelić-Ivanović, Zorana and Spasić, Slavica",
year = "2013",
abstract = "The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O-2(.-)), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O-2(.-) concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, although they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of AIDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O-2(.-) and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Biotechnology & Biotechnological Equipment",
title = "Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training",
volume = "27",
number = "3",
pages = "3843-3847",
doi = "10.5504/BBEQ.2012.0144"
}
Vujović, A., Spasojević-Kalimanovska, V., Bogavac-Stanojević, N., Kotur-Stevuljević, J., Sopić, M., Stefanović, A., Baralić, I., Đorđević, B., Jelić-Ivanović, Z.,& Spasić, S.. (2013). Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training. in Biotechnology & Biotechnological Equipment
Taylor & Francis Ltd, Abingdon., 27(3), 3843-3847.
https://doi.org/10.5504/BBEQ.2012.0144
Vujović A, Spasojević-Kalimanovska V, Bogavac-Stanojević N, Kotur-Stevuljević J, Sopić M, Stefanović A, Baralić I, Đorđević B, Jelić-Ivanović Z, Spasić S. Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training. in Biotechnology & Biotechnological Equipment. 2013;27(3):3843-3847.
doi:10.5504/BBEQ.2012.0144 .
