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dc.creatorVemić, Ana
dc.creatorJančić-Stojanović, Biljana
dc.creatorStamenković, Ivana
dc.creatorMalenović, Anđelija
dc.date.accessioned2019-09-02T11:34:29Z
dc.date.available2019-09-02T11:34:29Z
dc.date.issued2013
dc.identifier.issn0731-7085
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1927
dc.description.abstractThe simultaneous pharmaceutical analysis of multi-component drugs represents a challenge due to a large total number of analytes present in the sample. These analytes are not only the active pharmaceutical ingredients, but also the impurities that might follow the active substances. The aim of this study was to develop an efficient reversed-phase LC method for the simultaneous analysis of antiparkinsonian drugs levodopa, carbidopa and entacapone along with their six related impurities. For the achievement of desirable separation, different acids with anions possessing different properties according to Hofmeister classification (ortho-phosphoric, trifluoroacetic and perchloric acid) were tested. Finally, in order to draw the unbiased conclusions when optimizing the analytical method, for the final tuning of the gradient program, Box-Behnken experimental design and Derringer's desirability function were used. The experiments were performed on Zorbax Extend C18, 150 mm x 4.6 mm, 5 mu m particle size column with the UV detection at 280 nm and mobile phase flow rate of 1 mL/min. The optimal mobile phase consisted of methanol and 20 mM trifluoroacetic acid (pH 2.0 adjusted with NaOH), while their ratio is changed according to previously defined gradient program. The method was tested for selectivity, sensitivity, linearity, accuracy and precision, and proved to be suitable for routine qualitative and quantitative analysis of levodopa, carbidopa, entacapone and their impurities in their mixture.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Pharmaceutical and Biomedical Analysis
dc.subjectChaotropic agentsen
dc.subjectExperimental designen
dc.subjectGradient elutionen
dc.subjectAntiparkinsonian drugsen
dc.subjectImpuritiesen
dc.titleChaotropic agents in liquid chromatographic method development for the simultaneous analysis of levodopa, carbidopa, entacapone and their impuritiesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈанчић-Стојановић, Биљана; Вемић, Aна; Стаменковић, Ивана; Маленовић, Aнђелија;
dc.citation.volume77
dc.citation.spage9
dc.citation.epage15
dc.citation.other77: 9-15
dc.citation.rankM21
dc.identifier.wos000316306000002
dc.identifier.doi10.1016/j.jpba.2013.01.007
dc.identifier.pmid23354116
dc.identifier.scopus2-s2.0-84873046225
dc.identifier.rcubconv_2803
dc.type.versionpublishedVersion


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