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dc.creatorCrevar-Sakač, Milkica
dc.creatorVujić, Zorica
dc.creatorBrborić, Jasmina
dc.creatorKuntić, Vesna
dc.creatorUskoković-Marković, Snežana
dc.date.accessioned2019-09-02T11:34:33Z
dc.date.available2019-09-02T11:34:33Z
dc.date.issued2013
dc.identifier.issn1420-3049
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1928
dc.description.abstractThe aim of the present study was to optimize a chromatographic method for the analysis of atorvastatin (acid and lactone forms), ortho- and para-hydroxyatorvastatin by using an experimental design approach. Optimization experiments were conducted through a process of screening and optimization. The purpose of a screening design is to identify the factors that have significant effects on the selected chromatographic responses, and for this purpose a full 23 factorial design was used. The location of the true optimum was established by applying Derringer's desirability function, which provides simultaneously optimization of all seven responses. The ranges of the independent variables used for the optimization were content of acetonitrile in mobile phase (60-70%), temperature of column (30-40 C degrees) and flow rate (0.8-1.2 mL min(-1)). The influences of these independent variables were evaluated for the output responses: retention time of first peak (p-hydroxyatorvastatin) and of last peak (atorvastatin, lactone form), symmetries of all four peaks and relative retention time of p-hydroxyatorvastatin. The primary goal of this investigation was establishing a new simple and sensitive method that could be used in analysis of biological samples. The method was validated and successfully applied for determination of atorvastatin (acid and lactone forms) and its metabolites in plasma.en
dc.publisherMDPI, Basel
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172041/RS//
dc.rightsopenAccess
dc.sourceMolecules
dc.subjectatorvastatin (acid and lactone)en
dc.subjectortho- and para-hydroxyatorvastatinen
dc.subjectcentral composite designen
dc.subjectDerringer's desirability functionen
dc.subjectHPLCen
dc.titleAn Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasmaen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractЦревар-Сакач, Милкица; Ускоковић-Марковић, Снежана; Вујић, Зорица; Кунтић, Весна; Брборић, Јасмина;
dc.citation.volume18
dc.citation.issue3
dc.citation.spage2469
dc.citation.epage2482
dc.citation.other18(3): 2469-2482
dc.citation.rankM22
dc.identifier.wos000316611700002
dc.identifier.doi10.3390/molecules18032469
dc.identifier.pmid23439563
dc.identifier.scopus2-s2.0-84875606694
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/700/1926.pdf
dc.identifier.rcubconv_2806
dc.type.versionpublishedVersion


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