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An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol

Authorized Users Only
2013
Authors
Smajić, Miralem
Vujić, Zorica
Mulavdić, Nedzad
Brborić, Jasmina
Article (Published version)
Metadata
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Abstract
An experimental design method was used for fast, simple, and accurate high-performance-pressure liquid chromatograpy (HPLC) determination of losartan and hydrochlorothiazide in combined dosage forms. This method avoids the disadvantages of the traditional analytical approach, which is time-consuming, involves a large number of runs, and does not allow the determination of multiple interacting parameters. On the basis of preliminary experiments, three independent variables (methanol content, pH value of the mobile phase, and flow rate) were selected as input, and as dependent variables, five responses (retention time of hydrochlorothiazide, retention time of losartan, asymmetry of hydrochlorothiazide peak, asymmetry of losartan peak, and resolution) were chosen. A full 2(3) factorial design was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influencing parameters in the previously selected experimental do...main. After optimizing the experimental conditions, a separation was conducted on a Zorbax C-8 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-acetonitrile-acetate buffer 45:20:35 v/v/v, pH 4.8 with flow rate of 0.82 mL min(-1) and column temperature of 25 A degrees C. The developed method was successfully applied to simultaneous separation of these active drug compounds in their commercial pharmaceutical dosage forms.

Keywords:
HPLC / Experimental design / Losartan / Hydrochlorothiazide
Source:
Chromatographia, 2013, 76, 7-8, 419-425
Publisher:
  • Springer Heidelberg, Heidelberg
Funding / projects:
  • Development of molecules with antiinflammatory and cardioprotective activity: structural modifications, modelling, physicochemical characterization and formulation investigations (RS-172041)

DOI: 10.1007/s10337-013-2388-8

ISSN: 0009-5893

WoS: 000316819600016

Scopus: 2-s2.0-84878369036
[ Google Scholar ]
16
12
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/1930
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Smajić, Miralem
AU  - Vujić, Zorica
AU  - Mulavdić, Nedzad
AU  - Brborić, Jasmina
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1930
AB  - An experimental design method was used for fast, simple, and accurate high-performance-pressure liquid chromatograpy (HPLC) determination of losartan and hydrochlorothiazide in combined dosage forms. This method avoids the disadvantages of the traditional analytical approach, which is time-consuming, involves a large number of runs, and does not allow the determination of multiple interacting parameters. On the basis of preliminary experiments, three independent variables (methanol content, pH value of the mobile phase, and flow rate) were selected as input, and as dependent variables, five responses (retention time of hydrochlorothiazide, retention time of losartan, asymmetry of hydrochlorothiazide peak, asymmetry of losartan peak, and resolution) were chosen. A full 2(3) factorial design was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influencing parameters in the previously selected experimental domain. After optimizing the experimental conditions, a separation was conducted on a Zorbax C-8 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-acetonitrile-acetate buffer 45:20:35 v/v/v, pH 4.8 with flow rate of 0.82 mL min(-1) and column temperature of 25 A degrees C. The developed method was successfully applied to simultaneous separation of these active drug compounds in their commercial pharmaceutical dosage forms.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol
VL  - 76
IS  - 7-8
SP  - 419
EP  - 425
DO  - 10.1007/s10337-013-2388-8
ER  - 
@article{
author = "Smajić, Miralem and Vujić, Zorica and Mulavdić, Nedzad and Brborić, Jasmina",
year = "2013",
abstract = "An experimental design method was used for fast, simple, and accurate high-performance-pressure liquid chromatograpy (HPLC) determination of losartan and hydrochlorothiazide in combined dosage forms. This method avoids the disadvantages of the traditional analytical approach, which is time-consuming, involves a large number of runs, and does not allow the determination of multiple interacting parameters. On the basis of preliminary experiments, three independent variables (methanol content, pH value of the mobile phase, and flow rate) were selected as input, and as dependent variables, five responses (retention time of hydrochlorothiazide, retention time of losartan, asymmetry of hydrochlorothiazide peak, asymmetry of losartan peak, and resolution) were chosen. A full 2(3) factorial design was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influencing parameters in the previously selected experimental domain. After optimizing the experimental conditions, a separation was conducted on a Zorbax C-8 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-acetonitrile-acetate buffer 45:20:35 v/v/v, pH 4.8 with flow rate of 0.82 mL min(-1) and column temperature of 25 A degrees C. The developed method was successfully applied to simultaneous separation of these active drug compounds in their commercial pharmaceutical dosage forms.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol",
volume = "76",
number = "7-8",
pages = "419-425",
doi = "10.1007/s10337-013-2388-8"
}
Smajić, M., Vujić, Z., Mulavdić, N.,& Brborić, J.. (2013). An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol. in Chromatographia
Springer Heidelberg, Heidelberg., 76(7-8), 419-425.
https://doi.org/10.1007/s10337-013-2388-8
Smajić M, Vujić Z, Mulavdić N, Brborić J. An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol. in Chromatographia. 2013;76(7-8):419-425.
doi:10.1007/s10337-013-2388-8 .
Smajić, Miralem, Vujić, Zorica, Mulavdić, Nedzad, Brborić, Jasmina, "An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol" in Chromatographia, 76, no. 7-8 (2013):419-425,
https://doi.org/10.1007/s10337-013-2388-8 . .

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