The endogenous opioid system (EOS) plays a critical role in addictive processes. Molecular dysregulations in this system may be specific for different stages of addiction cycle and neurocircuitries involved and therefore may differentially contribute to the initiation and maintenance of addiction. Here we evaluated whether the EOS is altered in brain areas involved in cognitive control of addiction including the dorsolateral prefrontal cortex (dl-PFC), orbitofrontal cortex (OFC) and hippocampus in human alcohol-dependent subjects. Levels of EOS mRNAs were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and levels of dynorphins by radioimmunoassay (RIA) in post-mortem specimens obtained from 14 alcoholics and 14 controls. Prodynorphin mRNA and dynorphins in dl-PFC, ?-opioid receptor mRNA in OFC and dynorphins in hippocampus were up-regulated in alcoholics. No significant changes in expression of proenkephalin, and mu- and d-opioid receptors were evide...nt; pro-opiomelanocortin mRNA levels were below the detection limit. Activation of the ?-opioid receptor by up-regulated dynorphins in alcoholics may underlie in part neurocognitive dysfunctions relevant for addiction and disrupted inhibitory control.
Keywords:
Alcohol dependence / dynorphin / endogenous opioid system / human brain / -opioid receptor / prodynorphin
Source:
Addiction Biology, 2013, 18, 1, 161-169
Publisher:
Wiley-Blackwell, Hoboken
Funding / projects:
Swedish Council for Working Life and Social Research (FAS)
TY - JOUR
AU - Bazov, Igor
AU - Kononenko, Olga
AU - Watanabe, Hiroyuki
AU - Kuntić, Vesna
AU - Sarkisyan, Daniil
AU - Taqi, Malik M.
AU - Hussain, Muhammad Z.
AU - Nyberg, Fred
AU - Yakovleva, Tatjana
AU - Bakalkin, Georgy
PY - 2013
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1942
AB - The endogenous opioid system (EOS) plays a critical role in addictive processes. Molecular dysregulations in this system may be specific for different stages of addiction cycle and neurocircuitries involved and therefore may differentially contribute to the initiation and maintenance of addiction. Here we evaluated whether the EOS is altered in brain areas involved in cognitive control of addiction including the dorsolateral prefrontal cortex (dl-PFC), orbitofrontal cortex (OFC) and hippocampus in human alcohol-dependent subjects. Levels of EOS mRNAs were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and levels of dynorphins by radioimmunoassay (RIA) in post-mortem specimens obtained from 14 alcoholics and 14 controls. Prodynorphin mRNA and dynorphins in dl-PFC, ?-opioid receptor mRNA in OFC and dynorphins in hippocampus were up-regulated in alcoholics. No significant changes in expression of proenkephalin, and mu- and d-opioid receptors were evident; pro-opiomelanocortin mRNA levels were below the detection limit. Activation of the ?-opioid receptor by up-regulated dynorphins in alcoholics may underlie in part neurocognitive dysfunctions relevant for addiction and disrupted inhibitory control.
PB - Wiley-Blackwell, Hoboken
T2 - Addiction Biology
T1 - The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction
VL - 18
IS - 1
SP - 161
EP - 169
DO - 10.1111/j.1369-1600.2011.00366.x
ER -
@article{
author = "Bazov, Igor and Kononenko, Olga and Watanabe, Hiroyuki and Kuntić, Vesna and Sarkisyan, Daniil and Taqi, Malik M. and Hussain, Muhammad Z. and Nyberg, Fred and Yakovleva, Tatjana and Bakalkin, Georgy",
year = "2013",
abstract = "The endogenous opioid system (EOS) plays a critical role in addictive processes. Molecular dysregulations in this system may be specific for different stages of addiction cycle and neurocircuitries involved and therefore may differentially contribute to the initiation and maintenance of addiction. Here we evaluated whether the EOS is altered in brain areas involved in cognitive control of addiction including the dorsolateral prefrontal cortex (dl-PFC), orbitofrontal cortex (OFC) and hippocampus in human alcohol-dependent subjects. Levels of EOS mRNAs were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and levels of dynorphins by radioimmunoassay (RIA) in post-mortem specimens obtained from 14 alcoholics and 14 controls. Prodynorphin mRNA and dynorphins in dl-PFC, ?-opioid receptor mRNA in OFC and dynorphins in hippocampus were up-regulated in alcoholics. No significant changes in expression of proenkephalin, and mu- and d-opioid receptors were evident; pro-opiomelanocortin mRNA levels were below the detection limit. Activation of the ?-opioid receptor by up-regulated dynorphins in alcoholics may underlie in part neurocognitive dysfunctions relevant for addiction and disrupted inhibitory control.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Addiction Biology",
title = "The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction",
volume = "18",
number = "1",
pages = "161-169",
doi = "10.1111/j.1369-1600.2011.00366.x"
}
Bazov, I., Kononenko, O., Watanabe, H., Kuntić, V., Sarkisyan, D., Taqi, M. M., Hussain, M. Z., Nyberg, F., Yakovleva, T.,& Bakalkin, G.. (2013). The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction. in Addiction Biology
Wiley-Blackwell, Hoboken., 18(1), 161-169.
https://doi.org/10.1111/j.1369-1600.2011.00366.x
Bazov I, Kononenko O, Watanabe H, Kuntić V, Sarkisyan D, Taqi MM, Hussain MZ, Nyberg F, Yakovleva T, Bakalkin G. The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction. in Addiction Biology. 2013;18(1):161-169.
doi:10.1111/j.1369-1600.2011.00366.x .
Bazov, Igor, Kononenko, Olga, Watanabe, Hiroyuki, Kuntić, Vesna, Sarkisyan, Daniil, Taqi, Malik M., Hussain, Muhammad Z., Nyberg, Fred, Yakovleva, Tatjana, Bakalkin, Georgy, "The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction" in Addiction Biology, 18, no. 1 (2013):161-169,
https://doi.org/10.1111/j.1369-1600.2011.00366.x . .
