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dc.creatorKostić, Nada
dc.creatorDotsikas, Yannis
dc.creatorMalenović, Anđelija
dc.creatorJančić-Stojanović, Biljana
dc.creatorRakić, Tijana
dc.creatorIvanović, Darko
dc.creatorMedenica, Mirjana
dc.date.accessioned2019-09-02T11:35:31Z
dc.date.available2019-09-02T11:35:31Z
dc.date.issued2013
dc.identifier.issn1076-5174
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/1967
dc.description.abstractIn this article, a step-by-step optimization procedure for improving analyte response with implementation of experimental design is described. Zwitterionic antiepileptics, namely vigabatrin, pregabalin and gabapentin, were chosen as model compounds to undergo chloroformate-mediated derivatization followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis. Application of a planned stepwise optimization procedure allowed responses of analytes, expressed as areas and signal-to-noise ratios, to be improved, enabling achievement of lower limit of detection values. Results from the current study demonstrate that optimization of parameters such as scan time, geometry of ion source, sheath and auxiliary gas pressure, capillary temperature, collision pressure and mobile phase composition can have a positive impact on sensitivity of LC-MS/MS methods. Optimization of LC and MS parameters led to a total increment of 53.9%, 83.3% and 95.7% in areas of derivatized vigabatrin, pregabalin and gabapentin, respectively, while for signal-to-noise values, an improvement of 140.0%, 93.6% and 124.0% was achieved, compared to autotune settings. After defining the final optimal conditions, a time-segmented method was validated for the determination of mentioned drugs in plasma. The method proved to be accurate and precise with excellent linearity for the tested concentration range (40.0ngml(-1)-10.0x10(3)ngml(-1)). Copyrighten
dc.publisherWiley-Blackwell, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Mass Spectrometry
dc.subjectoptimizationen
dc.subjectantiepilepticsen
dc.subjection source parametersen
dc.subjectexperimental designen
dc.subjectresponse surface methodologyen
dc.titleStepwise optimization approach for improving LC-MS/MS analysis of zwitterionic antiepileptic drugs with implementation of experimental designen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРакић, Тијана; Ивановић, Дарко; Меденица, Мирјана; Костић, Нада; Маленовић, Aнђелија; Дотсикас, Yаннис; Јанчић-Стојановић, Биљана;
dc.citation.volume48
dc.citation.issue7
dc.citation.spage875
dc.citation.epage884
dc.citation.other48(7): 875-884
dc.citation.rankM21
dc.identifier.wos000321336000015
dc.identifier.doi10.1002/jms.3236
dc.identifier.pmid23832944
dc.identifier.scopus2-s2.0-84879922986
dc.type.versionpublishedVersion


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Приказ основних података о документу