HPTLC determination of ceftriaxone, cefixime and cefotaxime in dosage forms
Само за регистроване кориснике
1998
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The objective of this investigation was to develop a HPTLC method for the determination of ceftriaxone, cefixime and cefotaxime, cephalosporins widely used in clinical practice, High performance TLC of cephalosporins was performed on pre-coated silica gel HPTLC plates with concentrating zone (2.5 x 10 cm) by development in mobile phase ethyl acetate-acetone-methanol-water (5:2.5:2.5:1.5 v/v/v/v). A TLC scanner set at 270 nm was used for direct evaluation of the chromatograms in reflectance/absorbance mode. The calibration curves were established as dependence of peak height (linear and polynomial regression) and peak area (polynomial regression) versus ng level (125-500 ng for all cephalosporins investigated). Relative standard deviations obtained from calibration curves was compared. Precision (RSD: 1.12-2.91% (peak height versus ng) and RSD: 1.05-2.75% (peak area versus ng)), and detection limits (ng level) was validated and found to be satisfactory. The method was found to be reprod...ucible and convenient for quantitative analysis of ceftriaxone, cefixime and cefotaxime in their raw materials and their dosage forms.
Извор:
Journal of Pharmaceutical and Biomedical Analysis, 1998, 18, 4-5, 893-898Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/S0731-7085(98)00274-X
ISSN: 0731-7085
PubMed: 9919994
WoS: 000077971500049
Scopus: 2-s2.0-0032441868
Институција/група
PharmacyTY - JOUR AU - Erić-Jovanović, S AU - Agbaba, Danica AU - Živanov-Stakić, Dobrila AU - Vladimirov, S PY - 1998 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/197 AB - The objective of this investigation was to develop a HPTLC method for the determination of ceftriaxone, cefixime and cefotaxime, cephalosporins widely used in clinical practice, High performance TLC of cephalosporins was performed on pre-coated silica gel HPTLC plates with concentrating zone (2.5 x 10 cm) by development in mobile phase ethyl acetate-acetone-methanol-water (5:2.5:2.5:1.5 v/v/v/v). A TLC scanner set at 270 nm was used for direct evaluation of the chromatograms in reflectance/absorbance mode. The calibration curves were established as dependence of peak height (linear and polynomial regression) and peak area (polynomial regression) versus ng level (125-500 ng for all cephalosporins investigated). Relative standard deviations obtained from calibration curves was compared. Precision (RSD: 1.12-2.91% (peak height versus ng) and RSD: 1.05-2.75% (peak area versus ng)), and detection limits (ng level) was validated and found to be satisfactory. The method was found to be reproducible and convenient for quantitative analysis of ceftriaxone, cefixime and cefotaxime in their raw materials and their dosage forms. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - HPTLC determination of ceftriaxone, cefixime and cefotaxime in dosage forms VL - 18 IS - 4-5 SP - 893 EP - 898 DO - 10.1016/S0731-7085(98)00274-X ER -
@article{ author = "Erić-Jovanović, S and Agbaba, Danica and Živanov-Stakić, Dobrila and Vladimirov, S", year = "1998", abstract = "The objective of this investigation was to develop a HPTLC method for the determination of ceftriaxone, cefixime and cefotaxime, cephalosporins widely used in clinical practice, High performance TLC of cephalosporins was performed on pre-coated silica gel HPTLC plates with concentrating zone (2.5 x 10 cm) by development in mobile phase ethyl acetate-acetone-methanol-water (5:2.5:2.5:1.5 v/v/v/v). A TLC scanner set at 270 nm was used for direct evaluation of the chromatograms in reflectance/absorbance mode. The calibration curves were established as dependence of peak height (linear and polynomial regression) and peak area (polynomial regression) versus ng level (125-500 ng for all cephalosporins investigated). Relative standard deviations obtained from calibration curves was compared. Precision (RSD: 1.12-2.91% (peak height versus ng) and RSD: 1.05-2.75% (peak area versus ng)), and detection limits (ng level) was validated and found to be satisfactory. The method was found to be reproducible and convenient for quantitative analysis of ceftriaxone, cefixime and cefotaxime in their raw materials and their dosage forms.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "HPTLC determination of ceftriaxone, cefixime and cefotaxime in dosage forms", volume = "18", number = "4-5", pages = "893-898", doi = "10.1016/S0731-7085(98)00274-X" }
Erić-Jovanović, S., Agbaba, D., Živanov-Stakić, D.,& Vladimirov, S.. (1998). HPTLC determination of ceftriaxone, cefixime and cefotaxime in dosage forms. in Journal of Pharmaceutical and Biomedical Analysis Pergamon-Elsevier Science Ltd, Oxford., 18(4-5), 893-898. https://doi.org/10.1016/S0731-7085(98)00274-X
Erić-Jovanović S, Agbaba D, Živanov-Stakić D, Vladimirov S. HPTLC determination of ceftriaxone, cefixime and cefotaxime in dosage forms. in Journal of Pharmaceutical and Biomedical Analysis. 1998;18(4-5):893-898. doi:10.1016/S0731-7085(98)00274-X .
Erić-Jovanović, S, Agbaba, Danica, Živanov-Stakić, Dobrila, Vladimirov, S, "HPTLC determination of ceftriaxone, cefixime and cefotaxime in dosage forms" in Journal of Pharmaceutical and Biomedical Analysis, 18, no. 4-5 (1998):893-898, https://doi.org/10.1016/S0731-7085(98)00274-X . .