Thorough investigation of the retention mechanisms and retention behavior of amides and sulfonamides on amino column in hydrophilic interaction liquid chromatography
Само за регистроване кориснике
2013
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this paper detailed analysis of a mixture of four amides (tropicamide, nicotinamide, tiracetam, and piracetam) and six sulfonamides (sulfanilamide, sulfacetamide, sulfamethoxazole, sulfafurazole, furosemide, and bumetanide) on aminopropyl column in hydrophilic interaction chromatography (HILIC) was carried out. Since, there are no papers on the topic of the assessment of the contribution of ion-exchange retention mechanism involved in the separation of the acidic compounds on aminopropyl column in HILIC mode, the authors utilized the retention data of the acidic sulfonamides for this purpose. Next, broad range of the aqueous buffer concentrations in the mobile phase was examined providing the separation under either HILIC or RP conditions. Turning points between these two mechanisms were determined and then the fitting of the experimental data in the localized and non-localized adsorption models in both RP and HILIC regions was assessed. Since not many papers in the literature were ...dealing with the estimation of factor influence on the retention behavior of neutral and acidic compounds on aminopropyl column in HILIC, Box-Behnken design and Response Surface Methodology were applied. On the basis of the obtained data, ten quadratic models were proposed and their adequacy was confirmed using ANOVA test. Furthermore, retention data was graphically evaluated by the construction of 3D response surface plots. Finally, good predictive ability of the suggested models was proved with five additional verification experiments.
Кључне речи:
Hydrophilic interaction chromatography / Amides / Sulfonamides / Ion-exchange / Retention behavior / Box-Behnken designИзвор:
Journal of Chromatography A, 2013, 1301, 27-37Издавач:
- Elsevier Science BV, Amsterdam
Финансирање / пројекти:
- Моделовање различитих хроматографских система са хемометријским приступом у фармацеутској анализи (RS-MESTD-Basic Research (BR or ON)-172052)
DOI: 10.1016/j.chroma.2013.05.034
ISSN: 0021-9673
PubMed: 23791449
WoS: 000322098100004
Scopus: 2-s2.0-84879889158
Институција/група
PharmacyTY - JOUR AU - Jovanović, Marko AU - Jančić-Stojanović, Biljana PY - 2013 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1991 AB - In this paper detailed analysis of a mixture of four amides (tropicamide, nicotinamide, tiracetam, and piracetam) and six sulfonamides (sulfanilamide, sulfacetamide, sulfamethoxazole, sulfafurazole, furosemide, and bumetanide) on aminopropyl column in hydrophilic interaction chromatography (HILIC) was carried out. Since, there are no papers on the topic of the assessment of the contribution of ion-exchange retention mechanism involved in the separation of the acidic compounds on aminopropyl column in HILIC mode, the authors utilized the retention data of the acidic sulfonamides for this purpose. Next, broad range of the aqueous buffer concentrations in the mobile phase was examined providing the separation under either HILIC or RP conditions. Turning points between these two mechanisms were determined and then the fitting of the experimental data in the localized and non-localized adsorption models in both RP and HILIC regions was assessed. Since not many papers in the literature were dealing with the estimation of factor influence on the retention behavior of neutral and acidic compounds on aminopropyl column in HILIC, Box-Behnken design and Response Surface Methodology were applied. On the basis of the obtained data, ten quadratic models were proposed and their adequacy was confirmed using ANOVA test. Furthermore, retention data was graphically evaluated by the construction of 3D response surface plots. Finally, good predictive ability of the suggested models was proved with five additional verification experiments. PB - Elsevier Science BV, Amsterdam T2 - Journal of Chromatography A T1 - Thorough investigation of the retention mechanisms and retention behavior of amides and sulfonamides on amino column in hydrophilic interaction liquid chromatography VL - 1301 SP - 27 EP - 37 DO - 10.1016/j.chroma.2013.05.034 ER -
@article{ author = "Jovanović, Marko and Jančić-Stojanović, Biljana", year = "2013", abstract = "In this paper detailed analysis of a mixture of four amides (tropicamide, nicotinamide, tiracetam, and piracetam) and six sulfonamides (sulfanilamide, sulfacetamide, sulfamethoxazole, sulfafurazole, furosemide, and bumetanide) on aminopropyl column in hydrophilic interaction chromatography (HILIC) was carried out. Since, there are no papers on the topic of the assessment of the contribution of ion-exchange retention mechanism involved in the separation of the acidic compounds on aminopropyl column in HILIC mode, the authors utilized the retention data of the acidic sulfonamides for this purpose. Next, broad range of the aqueous buffer concentrations in the mobile phase was examined providing the separation under either HILIC or RP conditions. Turning points between these two mechanisms were determined and then the fitting of the experimental data in the localized and non-localized adsorption models in both RP and HILIC regions was assessed. Since not many papers in the literature were dealing with the estimation of factor influence on the retention behavior of neutral and acidic compounds on aminopropyl column in HILIC, Box-Behnken design and Response Surface Methodology were applied. On the basis of the obtained data, ten quadratic models were proposed and their adequacy was confirmed using ANOVA test. Furthermore, retention data was graphically evaluated by the construction of 3D response surface plots. Finally, good predictive ability of the suggested models was proved with five additional verification experiments.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of Chromatography A", title = "Thorough investigation of the retention mechanisms and retention behavior of amides and sulfonamides on amino column in hydrophilic interaction liquid chromatography", volume = "1301", pages = "27-37", doi = "10.1016/j.chroma.2013.05.034" }
Jovanović, M.,& Jančić-Stojanović, B.. (2013). Thorough investigation of the retention mechanisms and retention behavior of amides and sulfonamides on amino column in hydrophilic interaction liquid chromatography. in Journal of Chromatography A Elsevier Science BV, Amsterdam., 1301, 27-37. https://doi.org/10.1016/j.chroma.2013.05.034
Jovanović M, Jančić-Stojanović B. Thorough investigation of the retention mechanisms and retention behavior of amides and sulfonamides on amino column in hydrophilic interaction liquid chromatography. in Journal of Chromatography A. 2013;1301:27-37. doi:10.1016/j.chroma.2013.05.034 .
Jovanović, Marko, Jančić-Stojanović, Biljana, "Thorough investigation of the retention mechanisms and retention behavior of amides and sulfonamides on amino column in hydrophilic interaction liquid chromatography" in Journal of Chromatography A, 1301 (2013):27-37, https://doi.org/10.1016/j.chroma.2013.05.034 . .