Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography

2013
Authors
Jovanović, MarkoJančić-Stojanović, Biljana

Rakić, Tijana
Malenović, Anđelija

Ivanović, Darko
Medenica, Mirjana
Article (Published version)

Metadata
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Five different columns (two silica, two cyanopropyl and one diol) were investigated in hydrophilic interaction liquid chromatography (HILIC). For the assessment of columns behavior in HILIC mode, six basic drugs (lamotrigine, thioridazine, clozapine, chlorpheniramine, pheniramine and sulpiride) were chosen. The assessment of the influence of the concentration of organic modifier on analytes' retention on each column was provided by fitting the retention data into theoretical models. Utilizing the statistical analysis, the selection of the model that provides better prediction of the retention behavior was enabled. Dual RP-HILIC mechanism was suggested on cyanopropyl and diol columns, therefore the transition points between the two mechanisms on these columns were calculated. Furthermore, in order to investigate the impact of three factors simultaneously on the retention behavior of the analyzed substances on Betasil Silica column, chemometrically-aided empirical models were built. The ...experiments were conducted according to the matrix of Box-Behnken design and on the basis of the retention data, six quadratic models were obtained and their adequacy was confirmed using ANOVA test. The obtained coefficients of quadratic models enabled the elucidation of both single factor and factor interactions influence. This was also graphically presented in 3D response surface plots.
Keywords:
Hydrophilic interaction chromatography / Retention prediction / Retention behavior / Basic drugs / Box-Behnken designSource:
Central European Journal of Chemistry, 2013, 11, 7, 1150-1162Publisher:
- Versita, Warsaw
Funding / projects:
- Modelling of different chromatographic systems with chemometrical approach in pharmaceutical analysis (RS-172052)
DOI: 10.2478/s11532-013-0245-0
ISSN: 1895-1066
WoS: 000318180300012
Scopus: 2-s2.0-84881308170
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PharmacyTY - JOUR AU - Jovanović, Marko AU - Jančić-Stojanović, Biljana AU - Rakić, Tijana AU - Malenović, Anđelija AU - Ivanović, Darko AU - Medenica, Mirjana PY - 2013 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2001 AB - Five different columns (two silica, two cyanopropyl and one diol) were investigated in hydrophilic interaction liquid chromatography (HILIC). For the assessment of columns behavior in HILIC mode, six basic drugs (lamotrigine, thioridazine, clozapine, chlorpheniramine, pheniramine and sulpiride) were chosen. The assessment of the influence of the concentration of organic modifier on analytes' retention on each column was provided by fitting the retention data into theoretical models. Utilizing the statistical analysis, the selection of the model that provides better prediction of the retention behavior was enabled. Dual RP-HILIC mechanism was suggested on cyanopropyl and diol columns, therefore the transition points between the two mechanisms on these columns were calculated. Furthermore, in order to investigate the impact of three factors simultaneously on the retention behavior of the analyzed substances on Betasil Silica column, chemometrically-aided empirical models were built. The experiments were conducted according to the matrix of Box-Behnken design and on the basis of the retention data, six quadratic models were obtained and their adequacy was confirmed using ANOVA test. The obtained coefficients of quadratic models enabled the elucidation of both single factor and factor interactions influence. This was also graphically presented in 3D response surface plots. PB - Versita, Warsaw T2 - Central European Journal of Chemistry T1 - Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography VL - 11 IS - 7 SP - 1150 EP - 1162 DO - 10.2478/s11532-013-0245-0 ER -
@article{ author = "Jovanović, Marko and Jančić-Stojanović, Biljana and Rakić, Tijana and Malenović, Anđelija and Ivanović, Darko and Medenica, Mirjana", year = "2013", abstract = "Five different columns (two silica, two cyanopropyl and one diol) were investigated in hydrophilic interaction liquid chromatography (HILIC). For the assessment of columns behavior in HILIC mode, six basic drugs (lamotrigine, thioridazine, clozapine, chlorpheniramine, pheniramine and sulpiride) were chosen. The assessment of the influence of the concentration of organic modifier on analytes' retention on each column was provided by fitting the retention data into theoretical models. Utilizing the statistical analysis, the selection of the model that provides better prediction of the retention behavior was enabled. Dual RP-HILIC mechanism was suggested on cyanopropyl and diol columns, therefore the transition points between the two mechanisms on these columns were calculated. Furthermore, in order to investigate the impact of three factors simultaneously on the retention behavior of the analyzed substances on Betasil Silica column, chemometrically-aided empirical models were built. The experiments were conducted according to the matrix of Box-Behnken design and on the basis of the retention data, six quadratic models were obtained and their adequacy was confirmed using ANOVA test. The obtained coefficients of quadratic models enabled the elucidation of both single factor and factor interactions influence. This was also graphically presented in 3D response surface plots.", publisher = "Versita, Warsaw", journal = "Central European Journal of Chemistry", title = "Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography", volume = "11", number = "7", pages = "1150-1162", doi = "10.2478/s11532-013-0245-0" }
Jovanović, M., Jančić-Stojanović, B., Rakić, T., Malenović, A., Ivanović, D.,& Medenica, M.. (2013). Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography. in Central European Journal of Chemistry Versita, Warsaw., 11(7), 1150-1162. https://doi.org/10.2478/s11532-013-0245-0
Jovanović M, Jančić-Stojanović B, Rakić T, Malenović A, Ivanović D, Medenica M. Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography. in Central European Journal of Chemistry. 2013;11(7):1150-1162. doi:10.2478/s11532-013-0245-0 .
Jovanović, Marko, Jančić-Stojanović, Biljana, Rakić, Tijana, Malenović, Anđelija, Ivanović, Darko, Medenica, Mirjana, "Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography" in Central European Journal of Chemistry, 11, no. 7 (2013):1150-1162, https://doi.org/10.2478/s11532-013-0245-0 . .