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Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography

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2013
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Authors
Jovanović, Marko
Jančić-Stojanović, Biljana
Rakić, Tijana
Malenović, Anđelija
Ivanović, Darko
Medenica, Mirjana
Article (Published version)
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Abstract
Five different columns (two silica, two cyanopropyl and one diol) were investigated in hydrophilic interaction liquid chromatography (HILIC). For the assessment of columns behavior in HILIC mode, six basic drugs (lamotrigine, thioridazine, clozapine, chlorpheniramine, pheniramine and sulpiride) were chosen. The assessment of the influence of the concentration of organic modifier on analytes' retention on each column was provided by fitting the retention data into theoretical models. Utilizing the statistical analysis, the selection of the model that provides better prediction of the retention behavior was enabled. Dual RP-HILIC mechanism was suggested on cyanopropyl and diol columns, therefore the transition points between the two mechanisms on these columns were calculated. Furthermore, in order to investigate the impact of three factors simultaneously on the retention behavior of the analyzed substances on Betasil Silica column, chemometrically-aided empirical models were built. The ...experiments were conducted according to the matrix of Box-Behnken design and on the basis of the retention data, six quadratic models were obtained and their adequacy was confirmed using ANOVA test. The obtained coefficients of quadratic models enabled the elucidation of both single factor and factor interactions influence. This was also graphically presented in 3D response surface plots.

Keywords:
Hydrophilic interaction chromatography / Retention prediction / Retention behavior / Basic drugs / Box-Behnken design
Source:
Central European Journal of Chemistry, 2013, 11, 7, 1150-1162
Publisher:
  • Versita, Warsaw
Projects:
  • Modelling of different chromatographic systems with chemometrical approach in pharmaceutical analysis (RS-172052)

DOI: 10.2478/s11532-013-0245-0

ISSN: 1895-1066

WoS: 000318180300012

Scopus: 2-s2.0-84881308170
[ Google Scholar ]
9
9
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2001
Collections
  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy
TY  - JOUR
AU  - Jovanović, Marko
AU  - Jančić-Stojanović, Biljana
AU  - Rakić, Tijana
AU  - Malenović, Anđelija
AU  - Ivanović, Darko
AU  - Medenica, Mirjana
PY  - 2013
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2001
AB  - Five different columns (two silica, two cyanopropyl and one diol) were investigated in hydrophilic interaction liquid chromatography (HILIC). For the assessment of columns behavior in HILIC mode, six basic drugs (lamotrigine, thioridazine, clozapine, chlorpheniramine, pheniramine and sulpiride) were chosen. The assessment of the influence of the concentration of organic modifier on analytes' retention on each column was provided by fitting the retention data into theoretical models. Utilizing the statistical analysis, the selection of the model that provides better prediction of the retention behavior was enabled. Dual RP-HILIC mechanism was suggested on cyanopropyl and diol columns, therefore the transition points between the two mechanisms on these columns were calculated. Furthermore, in order to investigate the impact of three factors simultaneously on the retention behavior of the analyzed substances on Betasil Silica column, chemometrically-aided empirical models were built. The experiments were conducted according to the matrix of Box-Behnken design and on the basis of the retention data, six quadratic models were obtained and their adequacy was confirmed using ANOVA test. The obtained coefficients of quadratic models enabled the elucidation of both single factor and factor interactions influence. This was also graphically presented in 3D response surface plots.
PB  - Versita, Warsaw
T2  - Central European Journal of Chemistry
T1  - Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography
VL  - 11
IS  - 7
SP  - 1150
EP  - 1162
DO  - 10.2478/s11532-013-0245-0
ER  - 
@article{
author = "Jovanović, Marko and Jančić-Stojanović, Biljana and Rakić, Tijana and Malenović, Anđelija and Ivanović, Darko and Medenica, Mirjana",
year = "2013",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/2001",
abstract = "Five different columns (two silica, two cyanopropyl and one diol) were investigated in hydrophilic interaction liquid chromatography (HILIC). For the assessment of columns behavior in HILIC mode, six basic drugs (lamotrigine, thioridazine, clozapine, chlorpheniramine, pheniramine and sulpiride) were chosen. The assessment of the influence of the concentration of organic modifier on analytes' retention on each column was provided by fitting the retention data into theoretical models. Utilizing the statistical analysis, the selection of the model that provides better prediction of the retention behavior was enabled. Dual RP-HILIC mechanism was suggested on cyanopropyl and diol columns, therefore the transition points between the two mechanisms on these columns were calculated. Furthermore, in order to investigate the impact of three factors simultaneously on the retention behavior of the analyzed substances on Betasil Silica column, chemometrically-aided empirical models were built. The experiments were conducted according to the matrix of Box-Behnken design and on the basis of the retention data, six quadratic models were obtained and their adequacy was confirmed using ANOVA test. The obtained coefficients of quadratic models enabled the elucidation of both single factor and factor interactions influence. This was also graphically presented in 3D response surface plots.",
publisher = "Versita, Warsaw",
journal = "Central European Journal of Chemistry",
title = "Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography",
volume = "11",
number = "7",
pages = "1150-1162",
doi = "10.2478/s11532-013-0245-0"
}
Jovanović M, Jančić-Stojanović B, Rakić T, Malenović A, Ivanović D, Medenica M. Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography. Central European Journal of Chemistry. 2013;11(7):1150-1162
Jovanović, M., Jančić-Stojanović, B., Rakić, T., Malenović, A., Ivanović, D.,& Medenica, M. (2013). Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography.
Central European Journal of ChemistryVersita, Warsaw., 11(7), 1150-1162.
https://doi.org/10.2478/s11532-013-0245-0
Jovanović Marko, Jančić-Stojanović Biljana, Rakić Tijana, Malenović Anđelija, Ivanović Darko, Medenica Mirjana, "Five different columns in the analysis of basic drugs in hydrophilic interaction liquid chromatography" 11, no. 7 (2013):1150-1162,
https://doi.org/10.2478/s11532-013-0245-0 .

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