A reversed-phase high performance liquid chromatographic method for the determination of maprotilin in bulk drugs, ampoules and tablets is described. To achieve maximum quantity, optimum conditions were used. In order to investigate the effect of each factor and their interaction a three variable, two-level, full-factorial design was chosen. A response-surface diagram was developed to identify the optimum experimental conditions. Maximum response was obtained on a Bondesil C18 column (5μm × 4.6mm × 250mm) with acetonitril-methanol (pH adjusted 4.5 with 0.1 mol/l CH3COOH) mobile phase (1:1, v/v ) and UV detection (λ.=272nm). Peak area responses were linearly related to concentrations of maprotilin in the range 4μg/ml-60μg/ml. The regression equation is y= -1.06574 + 564.095x with correlation coefficient r=0.996. The reliability of the proposed method was checked up at three different concentrations (RSD varied from 0.592 to 2.675%). The described method applied for the determination of ...maprotilin in tablets and ampoules gave precise and reproducible results; recovery was 98.61% for tablets and 98.92% for ampoules. The developed method can be recommended for the routine analysis of maprotilin in tablets and ampoules.
Извор:
Journal de Pharmacie de Belgique, 1998, 53, 3, 267-
TY - JOUR
AU - Vujić, Zorica
AU - Radulović, Dušanka
PY - 1998
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/200
AB - A reversed-phase high performance liquid chromatographic method for the determination of maprotilin in bulk drugs, ampoules and tablets is described. To achieve maximum quantity, optimum conditions were used. In order to investigate the effect of each factor and their interaction a three variable, two-level, full-factorial design was chosen. A response-surface diagram was developed to identify the optimum experimental conditions. Maximum response was obtained on a Bondesil C18 column (5μm × 4.6mm × 250mm) with acetonitril-methanol (pH adjusted 4.5 with 0.1 mol/l CH3COOH) mobile phase (1:1, v/v ) and UV detection (λ.=272nm). Peak area responses were linearly related to concentrations of maprotilin in the range 4μg/ml-60μg/ml. The regression equation is y= -1.06574 + 564.095x with correlation coefficient r=0.996. The reliability of the proposed method was checked up at three different concentrations (RSD varied from 0.592 to 2.675%). The described method applied for the determination of maprotilin in tablets and ampoules gave precise and reproducible results; recovery was 98.61% for tablets and 98.92% for ampoules. The developed method can be recommended for the routine analysis of maprotilin in tablets and ampoules.
T2 - Journal de Pharmacie de Belgique
T1 - Statistical optimization applied to the HPLC determination of maprotilin
VL - 53
IS - 3
SP - 267
ER -
@article{
author = "Vujić, Zorica and Radulović, Dušanka",
year = "1998",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/200",
abstract = "A reversed-phase high performance liquid chromatographic method for the determination of maprotilin in bulk drugs, ampoules and tablets is described. To achieve maximum quantity, optimum conditions were used. In order to investigate the effect of each factor and their interaction a three variable, two-level, full-factorial design was chosen. A response-surface diagram was developed to identify the optimum experimental conditions. Maximum response was obtained on a Bondesil C18 column (5μm × 4.6mm × 250mm) with acetonitril-methanol (pH adjusted 4.5 with 0.1 mol/l CH3COOH) mobile phase (1:1, v/v ) and UV detection (λ.=272nm). Peak area responses were linearly related to concentrations of maprotilin in the range 4μg/ml-60μg/ml. The regression equation is y= -1.06574 + 564.095x with correlation coefficient r=0.996. The reliability of the proposed method was checked up at three different concentrations (RSD varied from 0.592 to 2.675%). The described method applied for the determination of maprotilin in tablets and ampoules gave precise and reproducible results; recovery was 98.61% for tablets and 98.92% for ampoules. The developed method can be recommended for the routine analysis of maprotilin in tablets and ampoules.",
journal = "Journal de Pharmacie de Belgique",
title = "Statistical optimization applied to the HPLC determination of maprotilin",
volume = "53",
number = "3",
pages = "267"
}
Vujić Z, Radulović D. Statistical optimization applied to the HPLC determination of maprotilin. Journal de Pharmacie de Belgique. 1998;53(3):267
Vujić, Z.,& Radulović, D. (1998). Statistical optimization applied to the HPLC determination of maprotilin.
Journal de Pharmacie de Belgique, 53(3), 267.
Vujić Zorica, Radulović Dušanka, "Statistical optimization applied to the HPLC determination of maprotilin" 53, no. 3 (1998):267
