Comparison of spectrophotometric and UV-densitometric methods for the determination of celiprolol hydrochloride in tablets

Abstract

Spectrophotometric and UV-densitometric methods for the determination of celiprolol hydrochloride in tablet formulations were compared. Celiprolol hydrochloride and benzylorange react in a 1:1 ratio in the presence of phosphate buffer, producing a yellow, chloroform-extractable ion pair with an absorption maximum at 401 nm. The conditional stability constants were determined by Sommer and Job's method of non-equimolar solutions. The repeatability of the method was tested by analysing Selectol tablets. Spectrophotometric results were compared with those obtained UV-densitometrically on Silica Gel GF 254 plates with acetone-methanol-triethylamine as mobile phase. There was no significant difference in accuracy between the two methods, however the densitometric method was more rapid.