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Carboxyhemoglobin in blood of smokers and non-smokers determined by gas chromatography with thermal conductivity detector

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Authors
Buha, Aleksandra
Vaseashta, A
Bulat, Zorica
Matović, Vesna
Article (Published version)
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Abstract
The aim of this study was to determine Carboxyhemoglobin (COHb) levels in the blood of smokers and non-smokers using gas chromatography; evaluate the impact of smoking habits; and determine whether living environment has influence on COHb blood content in non-smokers. Blood samples were collected from 127 Belgrade residents of different age and gender and smoking disposition (39 smokers and 88 non-smokers). The samples were analyzed by an Agilent Technologies 6890N gas chromatograph with molecular sieve packed column with thermal conductivity detector. A median value of COHb levels in non-smokers was 0.81 % and in smokers 5.33 %. Strong negative correlation (Pearson's r = -0.529) was obtained between COHb levels and time interval between last smoked cigarette and moment of blood sampling with significantly higher COHb levels in smokers who had their last cigarette ≤ 30 min before blood sampling. Statistically significant difference in COHb levels was obtained between residents living i...n the center of Belgrade, as compared to residents living in suburbs and outskirts of Belgrade with median values of 1.06 and 0.53 %, respectively. The obtained results suggest risk associated with first and second hand CO exposure. The method has greater applicability to test harmful exposure in polluted industries and detonation byproducts in battlefield and subject of nano-toxicity.

Keywords:
Blood-sampling / Carboxyhemoglobin / Gas chromatography / Nano-toxicity / Smokers
Source:
NATO Science for Peace and Security Series B: Physics and Biophysics, 2013, 163-171

DOI: 10.1007/978-94-007-7003-4-13

ISSN: 1874-6500

Scopus: 2-s2.0-84881393427
[ Google Scholar ]
2
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/2033
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Buha, Aleksandra
AU  - Vaseashta, A
AU  - Bulat, Zorica
AU  - Matović, Vesna
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2033
AB  - The aim of this study was to determine Carboxyhemoglobin (COHb) levels in the blood of smokers and non-smokers using gas chromatography; evaluate the impact of smoking habits; and determine whether living environment has influence on COHb blood content in non-smokers. Blood samples were collected from 127 Belgrade residents of different age and gender and smoking disposition (39 smokers and 88 non-smokers). The samples were analyzed by an Agilent Technologies 6890N gas chromatograph with molecular sieve packed column with thermal conductivity detector. A median value of COHb levels in non-smokers was 0.81 % and in smokers 5.33 %. Strong negative correlation (Pearson's r = -0.529) was obtained between COHb levels and time interval between last smoked cigarette and moment of blood sampling with significantly higher COHb levels in smokers who had their last cigarette ≤ 30 min before blood sampling. Statistically significant difference in COHb levels was obtained between residents living in the center of Belgrade, as compared to residents living in suburbs and outskirts of Belgrade with median values of 1.06 and 0.53 %, respectively. The obtained results suggest risk associated with first and second hand CO exposure. The method has greater applicability to test harmful exposure in polluted industries and detonation byproducts in battlefield and subject of nano-toxicity.
T2  - NATO Science for Peace and Security Series B: Physics and Biophysics
T1  - Carboxyhemoglobin in blood of smokers and non-smokers determined by gas chromatography with thermal conductivity detector
SP  - 163
EP  - 171
DO  - 10.1007/978-94-007-7003-4-13
ER  - 
@article{
author = "Buha, Aleksandra and Vaseashta, A and Bulat, Zorica and Matović, Vesna",
year = "2013",
abstract = "The aim of this study was to determine Carboxyhemoglobin (COHb) levels in the blood of smokers and non-smokers using gas chromatography; evaluate the impact of smoking habits; and determine whether living environment has influence on COHb blood content in non-smokers. Blood samples were collected from 127 Belgrade residents of different age and gender and smoking disposition (39 smokers and 88 non-smokers). The samples were analyzed by an Agilent Technologies 6890N gas chromatograph with molecular sieve packed column with thermal conductivity detector. A median value of COHb levels in non-smokers was 0.81 % and in smokers 5.33 %. Strong negative correlation (Pearson's r = -0.529) was obtained between COHb levels and time interval between last smoked cigarette and moment of blood sampling with significantly higher COHb levels in smokers who had their last cigarette ≤ 30 min before blood sampling. Statistically significant difference in COHb levels was obtained between residents living in the center of Belgrade, as compared to residents living in suburbs and outskirts of Belgrade with median values of 1.06 and 0.53 %, respectively. The obtained results suggest risk associated with first and second hand CO exposure. The method has greater applicability to test harmful exposure in polluted industries and detonation byproducts in battlefield and subject of nano-toxicity.",
journal = "NATO Science for Peace and Security Series B: Physics and Biophysics",
title = "Carboxyhemoglobin in blood of smokers and non-smokers determined by gas chromatography with thermal conductivity detector",
pages = "163-171",
doi = "10.1007/978-94-007-7003-4-13"
}
Buha, A., Vaseashta, A., Bulat, Z.,& Matović, V.. (2013). Carboxyhemoglobin in blood of smokers and non-smokers determined by gas chromatography with thermal conductivity detector. in NATO Science for Peace and Security Series B: Physics and Biophysics, 163-171.
https://doi.org/10.1007/978-94-007-7003-4-13
Buha A, Vaseashta A, Bulat Z, Matović V. Carboxyhemoglobin in blood of smokers and non-smokers determined by gas chromatography with thermal conductivity detector. in NATO Science for Peace and Security Series B: Physics and Biophysics. 2013;:163-171.
doi:10.1007/978-94-007-7003-4-13 .
Buha, Aleksandra, Vaseashta, A, Bulat, Zorica, Matović, Vesna, "Carboxyhemoglobin in blood of smokers and non-smokers determined by gas chromatography with thermal conductivity detector" in NATO Science for Peace and Security Series B: Physics and Biophysics (2013):163-171,
https://doi.org/10.1007/978-94-007-7003-4-13 . .

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