Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review
Abstract
Lyme borreliosis is the most common vector-borne disease in the northern hemisphere. The agents of Lyme borreliosis are borrelia, bacteria of the family Spirochaetaceae, which are grouped in Borrelia burgdorferi sensu lato species complex. Borreliae are fastidious, slow-growing and biochemically inactive bacteria that need special attention and optimal conditions for cultivation. The isolation of Borrelia from clinical material and their cultivation is a time-consuming and demanding procedure. Cultivation lasts from 9 up to 12 weeks, which is much longer than is necessary to grow most other human bacterial pathogens. Although B. burgdorferi sensu lato is susceptible to a wide range of antimicrobial agents in vitro, up to now the susceptibility of individual Borrelia species to antibiotics is defined only partially.
Keywords:
Borrelia / growth conditions in vitro / susceptibility testingSource:
Archives of Biological Sciences, 2013, 65, 2, 533-547Publisher:
- Srpsko biološko društvo, Beograd, i dr.
Funding / projects:
DOI: 10.2298/ABS1302533V
ISSN: 0354-4664
WoS: 000318826000016
Scopus: 2-s2.0-84876956938
Collections
Institution/Community
PharmacyTY - JOUR AU - Veinović, Gorana AU - Filipić, Brankica AU - Stanković, Jelena PY - 2013 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2067 AB - Lyme borreliosis is the most common vector-borne disease in the northern hemisphere. The agents of Lyme borreliosis are borrelia, bacteria of the family Spirochaetaceae, which are grouped in Borrelia burgdorferi sensu lato species complex. Borreliae are fastidious, slow-growing and biochemically inactive bacteria that need special attention and optimal conditions for cultivation. The isolation of Borrelia from clinical material and their cultivation is a time-consuming and demanding procedure. Cultivation lasts from 9 up to 12 weeks, which is much longer than is necessary to grow most other human bacterial pathogens. Although B. burgdorferi sensu lato is susceptible to a wide range of antimicrobial agents in vitro, up to now the susceptibility of individual Borrelia species to antibiotics is defined only partially. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review VL - 65 IS - 2 SP - 533 EP - 547 DO - 10.2298/ABS1302533V ER -
@article{ author = "Veinović, Gorana and Filipić, Brankica and Stanković, Jelena", year = "2013", abstract = "Lyme borreliosis is the most common vector-borne disease in the northern hemisphere. The agents of Lyme borreliosis are borrelia, bacteria of the family Spirochaetaceae, which are grouped in Borrelia burgdorferi sensu lato species complex. Borreliae are fastidious, slow-growing and biochemically inactive bacteria that need special attention and optimal conditions for cultivation. The isolation of Borrelia from clinical material and their cultivation is a time-consuming and demanding procedure. Cultivation lasts from 9 up to 12 weeks, which is much longer than is necessary to grow most other human bacterial pathogens. Although B. burgdorferi sensu lato is susceptible to a wide range of antimicrobial agents in vitro, up to now the susceptibility of individual Borrelia species to antibiotics is defined only partially.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review", volume = "65", number = "2", pages = "533-547", doi = "10.2298/ABS1302533V" }
Veinović, G., Filipić, B.,& Stanković, J.. (2013). Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 65(2), 533-547. https://doi.org/10.2298/ABS1302533V
Veinović G, Filipić B, Stanković J. Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review. in Archives of Biological Sciences. 2013;65(2):533-547. doi:10.2298/ABS1302533V .
Veinović, Gorana, Filipić, Brankica, Stanković, Jelena, "Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review" in Archives of Biological Sciences, 65, no. 2 (2013):533-547, https://doi.org/10.2298/ABS1302533V . .