Antibacterial activity of three newly-synthesized chalcones & synergism with antibiotics against clinical isolates of methicillin-resistant Staphylococcus aureus
Apstrakt
Background & objectives: Multidrug-resistance of methicillin-resistant Staphylococcus aureus (MRSA) is a serious therapeutical problem. Chalcones belong to a group of naturally occurring flavonoids, usually found in various plant species, and have potent antibacterial, antiviral and antifungal activities. The goal of this study was to evaluate the antibacterial effect of three newly-synthesized chalcones against clinical isolates of MRSA, and their synergism with beta-lactam and non-beta-lactam antibiotics. Methods: Antimicrobial activity of the three newly-synthesized chalcones was tested against 19 clinical isolates of MRSA and a laboratory control strain of MRSA (ATCC 43300). The synergism with beta-lactams: cefotaxime (CFX), ceftriaxone (CTX), and non-beta-lactam antibiotics: ciprofloxacin (CIP), gentamicin (GEN) and trimethoprim/sulphamethoxazole (TMP-SMX) was investigated by checkerboard method. Results: All evaluated compounds showed significant anti-MRSA activity with MIC value...s from 25-200 g/ml. Observed synergism with antibiotics demonstrated that chalcones significantly enhanced the efficacy of CIP, GEN and TMP-SMX. Interpretation & conclusions: our study demonstrated that three newly-synthesized chalcones exhibited significant anti-MRSA effect and synergism with non-beta-lactam antibiotics. The most effective compound was 1,3-Bis-(2-hydroxy-phenyl)-propenone. Our results provide useful information for future research of possible application of chalcones in combination with conventional anti-MRSA therapy as promising new antimicrobial agents.
Ključne reči:
Chalcones / checkerboard method / MRSA / multidrug-resistance / synergyIzvor:
Indian Journal of Medical Research, 2014, 140, 130-137Izdavač:
- Indian Council Medical Res, New Delhi
Finansiranje / projekti:
- Bakterije rezistentne na antibiotike u Srbiji: fenotipska i genotipska karakterizacija (RS-MESTD-Basic Research (BR or ON)-175039)
Institucija/grupa
PharmacyTY - JOUR AU - Božić, Dragana AU - Milenković, Marina AU - Ivković, Branka AU - Cirković, Ivana PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2071 AB - Background & objectives: Multidrug-resistance of methicillin-resistant Staphylococcus aureus (MRSA) is a serious therapeutical problem. Chalcones belong to a group of naturally occurring flavonoids, usually found in various plant species, and have potent antibacterial, antiviral and antifungal activities. The goal of this study was to evaluate the antibacterial effect of three newly-synthesized chalcones against clinical isolates of MRSA, and their synergism with beta-lactam and non-beta-lactam antibiotics. Methods: Antimicrobial activity of the three newly-synthesized chalcones was tested against 19 clinical isolates of MRSA and a laboratory control strain of MRSA (ATCC 43300). The synergism with beta-lactams: cefotaxime (CFX), ceftriaxone (CTX), and non-beta-lactam antibiotics: ciprofloxacin (CIP), gentamicin (GEN) and trimethoprim/sulphamethoxazole (TMP-SMX) was investigated by checkerboard method. Results: All evaluated compounds showed significant anti-MRSA activity with MIC values from 25-200 g/ml. Observed synergism with antibiotics demonstrated that chalcones significantly enhanced the efficacy of CIP, GEN and TMP-SMX. Interpretation & conclusions: our study demonstrated that three newly-synthesized chalcones exhibited significant anti-MRSA effect and synergism with non-beta-lactam antibiotics. The most effective compound was 1,3-Bis-(2-hydroxy-phenyl)-propenone. Our results provide useful information for future research of possible application of chalcones in combination with conventional anti-MRSA therapy as promising new antimicrobial agents. PB - Indian Council Medical Res, New Delhi T2 - Indian Journal of Medical Research T1 - Antibacterial activity of three newly-synthesized chalcones & synergism with antibiotics against clinical isolates of methicillin-resistant Staphylococcus aureus VL - 140 SP - 130 EP - 137 UR - https://hdl.handle.net/21.15107/rcub_farfar_2071 ER -
@article{ author = "Božić, Dragana and Milenković, Marina and Ivković, Branka and Cirković, Ivana", year = "2014", abstract = "Background & objectives: Multidrug-resistance of methicillin-resistant Staphylococcus aureus (MRSA) is a serious therapeutical problem. Chalcones belong to a group of naturally occurring flavonoids, usually found in various plant species, and have potent antibacterial, antiviral and antifungal activities. The goal of this study was to evaluate the antibacterial effect of three newly-synthesized chalcones against clinical isolates of MRSA, and their synergism with beta-lactam and non-beta-lactam antibiotics. Methods: Antimicrobial activity of the three newly-synthesized chalcones was tested against 19 clinical isolates of MRSA and a laboratory control strain of MRSA (ATCC 43300). The synergism with beta-lactams: cefotaxime (CFX), ceftriaxone (CTX), and non-beta-lactam antibiotics: ciprofloxacin (CIP), gentamicin (GEN) and trimethoprim/sulphamethoxazole (TMP-SMX) was investigated by checkerboard method. Results: All evaluated compounds showed significant anti-MRSA activity with MIC values from 25-200 g/ml. Observed synergism with antibiotics demonstrated that chalcones significantly enhanced the efficacy of CIP, GEN and TMP-SMX. Interpretation & conclusions: our study demonstrated that three newly-synthesized chalcones exhibited significant anti-MRSA effect and synergism with non-beta-lactam antibiotics. The most effective compound was 1,3-Bis-(2-hydroxy-phenyl)-propenone. Our results provide useful information for future research of possible application of chalcones in combination with conventional anti-MRSA therapy as promising new antimicrobial agents.", publisher = "Indian Council Medical Res, New Delhi", journal = "Indian Journal of Medical Research", title = "Antibacterial activity of three newly-synthesized chalcones & synergism with antibiotics against clinical isolates of methicillin-resistant Staphylococcus aureus", volume = "140", pages = "130-137", url = "https://hdl.handle.net/21.15107/rcub_farfar_2071" }
Božić, D., Milenković, M., Ivković, B.,& Cirković, I.. (2014). Antibacterial activity of three newly-synthesized chalcones & synergism with antibiotics against clinical isolates of methicillin-resistant Staphylococcus aureus. in Indian Journal of Medical Research Indian Council Medical Res, New Delhi., 140, 130-137. https://hdl.handle.net/21.15107/rcub_farfar_2071
Božić D, Milenković M, Ivković B, Cirković I. Antibacterial activity of three newly-synthesized chalcones & synergism with antibiotics against clinical isolates of methicillin-resistant Staphylococcus aureus. in Indian Journal of Medical Research. 2014;140:130-137. https://hdl.handle.net/21.15107/rcub_farfar_2071 .
Božić, Dragana, Milenković, Marina, Ivković, Branka, Cirković, Ivana, "Antibacterial activity of three newly-synthesized chalcones & synergism with antibiotics against clinical isolates of methicillin-resistant Staphylococcus aureus" in Indian Journal of Medical Research, 140 (2014):130-137, https://hdl.handle.net/21.15107/rcub_farfar_2071 .