Chloroform Extract of Underground Parts of Ferula heuffelii: Secondary Metabolites and Spasmolytic Activity
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2014
Authors
Pavlović, IvanKrunić, Aleksej
Nikolić, Dejan
Radenković, Mirjana
Branković, Suzana
Niketić, Marjan

Petrović, Silvana

Article (Published version)

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Plants from the genus Ferula L. (Apiaceae) were used for various purposes in traditional medicine of different nations throughout the history. Ferula heuffelii GRISEB. ex HEUFFEL is a perennial species endemic for Balkan peninsula. Ten compounds which belong to classes of prenyl-furocoumarin-, prenyl-dihydrofurochromone-, prenyl-benzoyl-and prenyl-benzoylfuranone-type sesquiterpenoids, as well as sesquiterpene coumarins and phenylpropanoids, were, for the first time, isolated from the CHCl3 extract of the underground parts of this plant and identified. Furthermore, extract and three isolated compounds, i.e., latifolone (1), dshamirone (4), and (2S*, 3R*)-2-[(3E)-4,8-dimethylnona-3,7-dien-1-yl]-2,3-dihydro7- hydroxy-2,3-dimethylfuro[3,2-c] coumarin (6) were, for the first time, evaluated for their in vitro antispasmodic activities in three experimental models: spontaneous contraction, and ACh- and KCl-induced contraction of an isolated rat ileum. The extract (0.1-1.3 mg/ml) and compound... 6 (1 - 10 mu g/ml) exhibited dose-dependent effect in all three models. Compound 1 (1-6 mu g/ml) affected spontaneous contractions and those induced by KCl, while compound 4 (8 mu g/ml) displayed only moderate activity with ACh-induced contractions. It can be concluded that tested compounds contribute to exhibited antispasmodic activity of crude extract. Additionally, extract (0.1-1.3 mg/ml) was tested for in vitro relaxant activity on an isolated rat trachea, and relaxed the KCl-induced contractions in a dose-dependent manner.
Source:
Chemistry & Biodiversity, 2014, 11, 9, 1417-1427Publisher:
- Wiley-VCH Verlag GMBH, Weinheim
Funding / projects:
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-173021)
DOI: 10.1002/cbdv.201400094
ISSN: 1612-1872
PubMed: 25238082
WoS: 000342625500011
Scopus: 2-s2.0-84925953269
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PharmacyTY - JOUR AU - Pavlović, Ivan AU - Krunić, Aleksej AU - Nikolić, Dejan AU - Radenković, Mirjana AU - Branković, Suzana AU - Niketić, Marjan AU - Petrović, Silvana PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2077 AB - Plants from the genus Ferula L. (Apiaceae) were used for various purposes in traditional medicine of different nations throughout the history. Ferula heuffelii GRISEB. ex HEUFFEL is a perennial species endemic for Balkan peninsula. Ten compounds which belong to classes of prenyl-furocoumarin-, prenyl-dihydrofurochromone-, prenyl-benzoyl-and prenyl-benzoylfuranone-type sesquiterpenoids, as well as sesquiterpene coumarins and phenylpropanoids, were, for the first time, isolated from the CHCl3 extract of the underground parts of this plant and identified. Furthermore, extract and three isolated compounds, i.e., latifolone (1), dshamirone (4), and (2S*, 3R*)-2-[(3E)-4,8-dimethylnona-3,7-dien-1-yl]-2,3-dihydro7- hydroxy-2,3-dimethylfuro[3,2-c] coumarin (6) were, for the first time, evaluated for their in vitro antispasmodic activities in three experimental models: spontaneous contraction, and ACh- and KCl-induced contraction of an isolated rat ileum. The extract (0.1-1.3 mg/ml) and compound 6 (1 - 10 mu g/ml) exhibited dose-dependent effect in all three models. Compound 1 (1-6 mu g/ml) affected spontaneous contractions and those induced by KCl, while compound 4 (8 mu g/ml) displayed only moderate activity with ACh-induced contractions. It can be concluded that tested compounds contribute to exhibited antispasmodic activity of crude extract. Additionally, extract (0.1-1.3 mg/ml) was tested for in vitro relaxant activity on an isolated rat trachea, and relaxed the KCl-induced contractions in a dose-dependent manner. PB - Wiley-VCH Verlag GMBH, Weinheim T2 - Chemistry & Biodiversity T1 - Chloroform Extract of Underground Parts of Ferula heuffelii: Secondary Metabolites and Spasmolytic Activity VL - 11 IS - 9 SP - 1417 EP - 1427 DO - 10.1002/cbdv.201400094 ER -
@article{ author = "Pavlović, Ivan and Krunić, Aleksej and Nikolić, Dejan and Radenković, Mirjana and Branković, Suzana and Niketić, Marjan and Petrović, Silvana", year = "2014", abstract = "Plants from the genus Ferula L. (Apiaceae) were used for various purposes in traditional medicine of different nations throughout the history. Ferula heuffelii GRISEB. ex HEUFFEL is a perennial species endemic for Balkan peninsula. Ten compounds which belong to classes of prenyl-furocoumarin-, prenyl-dihydrofurochromone-, prenyl-benzoyl-and prenyl-benzoylfuranone-type sesquiterpenoids, as well as sesquiterpene coumarins and phenylpropanoids, were, for the first time, isolated from the CHCl3 extract of the underground parts of this plant and identified. Furthermore, extract and three isolated compounds, i.e., latifolone (1), dshamirone (4), and (2S*, 3R*)-2-[(3E)-4,8-dimethylnona-3,7-dien-1-yl]-2,3-dihydro7- hydroxy-2,3-dimethylfuro[3,2-c] coumarin (6) were, for the first time, evaluated for their in vitro antispasmodic activities in three experimental models: spontaneous contraction, and ACh- and KCl-induced contraction of an isolated rat ileum. The extract (0.1-1.3 mg/ml) and compound 6 (1 - 10 mu g/ml) exhibited dose-dependent effect in all three models. Compound 1 (1-6 mu g/ml) affected spontaneous contractions and those induced by KCl, while compound 4 (8 mu g/ml) displayed only moderate activity with ACh-induced contractions. It can be concluded that tested compounds contribute to exhibited antispasmodic activity of crude extract. Additionally, extract (0.1-1.3 mg/ml) was tested for in vitro relaxant activity on an isolated rat trachea, and relaxed the KCl-induced contractions in a dose-dependent manner.", publisher = "Wiley-VCH Verlag GMBH, Weinheim", journal = "Chemistry & Biodiversity", title = "Chloroform Extract of Underground Parts of Ferula heuffelii: Secondary Metabolites and Spasmolytic Activity", volume = "11", number = "9", pages = "1417-1427", doi = "10.1002/cbdv.201400094" }
Pavlović, I., Krunić, A., Nikolić, D., Radenković, M., Branković, S., Niketić, M.,& Petrović, S.. (2014). Chloroform Extract of Underground Parts of Ferula heuffelii: Secondary Metabolites and Spasmolytic Activity. in Chemistry & Biodiversity Wiley-VCH Verlag GMBH, Weinheim., 11(9), 1417-1427. https://doi.org/10.1002/cbdv.201400094
Pavlović I, Krunić A, Nikolić D, Radenković M, Branković S, Niketić M, Petrović S. Chloroform Extract of Underground Parts of Ferula heuffelii: Secondary Metabolites and Spasmolytic Activity. in Chemistry & Biodiversity. 2014;11(9):1417-1427. doi:10.1002/cbdv.201400094 .
Pavlović, Ivan, Krunić, Aleksej, Nikolić, Dejan, Radenković, Mirjana, Branković, Suzana, Niketić, Marjan, Petrović, Silvana, "Chloroform Extract of Underground Parts of Ferula heuffelii: Secondary Metabolites and Spasmolytic Activity" in Chemistry & Biodiversity, 11, no. 9 (2014):1417-1427, https://doi.org/10.1002/cbdv.201400094 . .