A kinetic method for the determination of selenium(IV) traces is proposed, based on its inhibitory action on the oxidation of Nile Blue A by hydrogen peroxide in phosphate buffer (pH 10.5). A linear dependence was established between the rate of the proposed indicator reaction and selenium concentration in the range 9.5 × 10 -2 -1.58 ng cm -3 . The experimental conditions of maximal selenium effect were established. Selenium, determined by the tangent method, was determined at concentrations over the range 0.22-1.26 ng cm -3 , with relative standard deviations up to 4.5%. The reaction rate was followed spectrophotometrically. The effect of foreign ions on the accuracy of this method was also investigated. The method was applied to the determination of selenium in pharmaceutical preparations and wheat flour.
Ključne reči:
Hydrogen peroxide / Kinetic determination / Nile Blue A oxidation / Pharmaceutical preparations / Selenium(IV) / Total selenium / Wheat flour
TY - JOUR
AU - Milovanović, G.A
AU - Petronijević Mira, R.B
AU - Čakar, Mira
PY - 1998
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/207
AB - A kinetic method for the determination of selenium(IV) traces is proposed, based on its inhibitory action on the oxidation of Nile Blue A by hydrogen peroxide in phosphate buffer (pH 10.5). A linear dependence was established between the rate of the proposed indicator reaction and selenium concentration in the range 9.5 × 10 -2 -1.58 ng cm -3 . The experimental conditions of maximal selenium effect were established. Selenium, determined by the tangent method, was determined at concentrations over the range 0.22-1.26 ng cm -3 , with relative standard deviations up to 4.5%. The reaction rate was followed spectrophotometrically. The effect of foreign ions on the accuracy of this method was also investigated. The method was applied to the determination of selenium in pharmaceutical preparations and wheat flour.
PB - Springer New York
T2 - Mikrochimica Acta
T1 - Kinetic Determination of Trace Levels of Selenium(IV) and Total Selenium by Nile Blue A/Hydrogen Peroxide Method
VL - 128
IS - 1
SP - 43
EP - 48
ER -
@article{
author = "Milovanović, G.A and Petronijević Mira, R.B and Čakar, Mira",
year = "1998",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/207",
abstract = "A kinetic method for the determination of selenium(IV) traces is proposed, based on its inhibitory action on the oxidation of Nile Blue A by hydrogen peroxide in phosphate buffer (pH 10.5). A linear dependence was established between the rate of the proposed indicator reaction and selenium concentration in the range 9.5 × 10 -2 -1.58 ng cm -3 . The experimental conditions of maximal selenium effect were established. Selenium, determined by the tangent method, was determined at concentrations over the range 0.22-1.26 ng cm -3 , with relative standard deviations up to 4.5%. The reaction rate was followed spectrophotometrically. The effect of foreign ions on the accuracy of this method was also investigated. The method was applied to the determination of selenium in pharmaceutical preparations and wheat flour.",
publisher = "Springer New York",
journal = "Mikrochimica Acta",
title = "Kinetic Determination of Trace Levels of Selenium(IV) and Total Selenium by Nile Blue A/Hydrogen Peroxide Method",
volume = "128",
number = "1",
pages = "43-48"
}
Milovanović G, Petronijević Mira R, Čakar M. Kinetic Determination of Trace Levels of Selenium(IV) and Total Selenium by Nile Blue A/Hydrogen Peroxide Method. Mikrochimica Acta. 1998;128(1):43-48
Milovanović, G.A, Petronijević Mira, R.B,& Čakar, M. (1998). Kinetic Determination of Trace Levels of Selenium(IV) and Total Selenium by Nile Blue A/Hydrogen Peroxide Method.
Mikrochimica ActaSpringer New York., 128(1), 43-48.
Milovanović G.A, Petronijević Mira R.B, Čakar Mira, "Kinetic Determination of Trace Levels of Selenium(IV) and Total Selenium by Nile Blue A/Hydrogen Peroxide Method" 128, no. 1 (1998):43-48
