High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.
Keywords:
Free bile acids / HPLC-ELSD / Pharmaceuticals / TLC-FID
TY - JOUR
AU - Novaković, J
AU - Tvrzická, E
AU - Ražić, Slavica
PY - 1998
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/208
AB - High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.
PB - Lubner Communication Srl
T2 - Bollettino chimico farmaceutico
T1 - Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography
VL - 137
IS - 10
SP - 412
EP - 416
ER -
@article{
author = "Novaković, J and Tvrzická, E and Ražić, Slavica",
year = "1998",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/208",
abstract = "High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.",
publisher = "Lubner Communication Srl",
journal = "Bollettino chimico farmaceutico",
title = "Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography",
volume = "137",
number = "10",
pages = "412-416"
}
Novaković J, Tvrzická E, Ražić S. Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography. Bollettino chimico farmaceutico. 1998;137(10):412-416
Novaković, J., Tvrzická, E.,& Ražić, S. (1998). Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography.
Bollettino chimico farmaceuticoLubner Communication Srl., 137(10), 412-416.
Novaković J, Tvrzická E, Ražić Slavica, "Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography" 137, no. 10 (1998):412-416
