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Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography

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Authors
Novaković, J
Tvrzická, E
Ražić, Slavica
Article (Published version)
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Abstract
High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.
Keywords:
Free bile acids / HPLC-ELSD / Pharmaceuticals / TLC-FID
Source:
Bollettino chimico farmaceutico, 1998, 137, 10, 412-416
Publisher:
  • Lubner Communication Srl

ISSN: 0006-6648

Scopus: 2-s2.0-0032435597
[ Google Scholar ]
3
Handle
https://hdl.handle.net/21.15107/rcub_farfar_208
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/208
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Novaković, J
AU  - Tvrzická, E
AU  - Ražić, Slavica
PY  - 1998
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/208
AB  - High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.
PB  - Lubner Communication Srl
T2  - Bollettino chimico farmaceutico
T1  - Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography
VL  - 137
IS  - 10
SP  - 412
EP  - 416
UR  - https://hdl.handle.net/21.15107/rcub_farfar_208
ER  - 
@article{
author = "Novaković, J and Tvrzická, E and Ražić, Slavica",
year = "1998",
abstract = "High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.",
publisher = "Lubner Communication Srl",
journal = "Bollettino chimico farmaceutico",
title = "Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography",
volume = "137",
number = "10",
pages = "412-416",
url = "https://hdl.handle.net/21.15107/rcub_farfar_208"
}
Novaković, J., Tvrzická, E.,& Ražić, S.. (1998). Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography. in Bollettino chimico farmaceutico
Lubner Communication Srl., 137(10), 412-416.
https://hdl.handle.net/21.15107/rcub_farfar_208
Novaković J, Tvrzická E, Ražić S. Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography. in Bollettino chimico farmaceutico. 1998;137(10):412-416.
https://hdl.handle.net/21.15107/rcub_farfar_208 .
Novaković, J, Tvrzická, E, Ražić, Slavica, "Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography" in Bollettino chimico farmaceutico, 137, no. 10 (1998):412-416,
https://hdl.handle.net/21.15107/rcub_farfar_208 .

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