Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography
Abstract
High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.
Keywords:
Free bile acids / HPLC-ELSD / Pharmaceuticals / TLC-FIDSource:
Bollettino chimico farmaceutico, 1998, 137, 10, 412-416Publisher:
- Lubner Communication Srl
Collections
Institution
PharmacyTY - JOUR AU - Novaković, J AU - Tvrzická, E AU - Ražić, Slavica PY - 1998 UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/208 AB - High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities. PB - Lubner Communication Srl T2 - Bollettino chimico farmaceutico T1 - Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography VL - 137 IS - 10 SP - 412 EP - 416 ER -
@article{ author = "Novaković, J and Tvrzická, E and Ražić, Slavica", year = "1998", url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/208", abstract = "High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.", publisher = "Lubner Communication Srl", journal = "Bollettino chimico farmaceutico", title = "Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography", volume = "137", number = "10", pages = "412-416" }
Novaković J, Tvrzická E, Ražić S. Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography. Bollettino chimico farmaceutico. 1998;137(10):412-416
Novaković, J., Tvrzická, E.,& Ražić, S. (1998). Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography. Bollettino chimico farmaceuticoLubner Communication Srl., 137(10), 412-416.
Novaković J, Tvrzická E, Ražić Slavica, "Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography" 137, no. 10 (1998):412-416