FarFaR - Farmaceutski fakultet, repozitorijum
Univerzitet u Beogradu, Farmaceutski fakultet
    • English
    • Српски
    • Српски (Serbia)
  • Srpski (latinica) 
    • Engleski
    • Srpski (ćirilica)
    • Srpski (latinica)
  • Prijava
Pregled zapisa 
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • Pregled zapisa
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • Pregled zapisa
JavaScript is disabled for your browser. Some features of this site may not work without it.

Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography

Nema prikaza
Autori
Novaković, J
Tvrzická, E
Ražić, Slavica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.
Ključne reči:
Free bile acids / HPLC-ELSD / Pharmaceuticals / TLC-FID
Izvor:
Bollettino chimico farmaceutico, 1998, 137, 10, 412-416
Izdavač:
  • Lubner Communication Srl

ISSN: 0006-6648

Scopus: 2-s2.0-0032435597
[ Google Scholar ]
3
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/208
Kolekcije
  • Radovi istraživača / Researchers’ publications
Institucija/grupa
Pharmacy
TY  - JOUR
AU  - Novaković, J
AU  - Tvrzická, E
AU  - Ražić, Slavica
PY  - 1998
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/208
AB  - High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.
PB  - Lubner Communication Srl
T2  - Bollettino chimico farmaceutico
T1  - Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography
VL  - 137
IS  - 10
SP  - 412
EP  - 416
UR  - conv_5356
ER  - 
@article{
author = "Novaković, J and Tvrzická, E and Ražić, Slavica",
year = "1998",
abstract = "High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.",
publisher = "Lubner Communication Srl",
journal = "Bollettino chimico farmaceutico",
title = "Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography",
volume = "137",
number = "10",
pages = "412-416",
url = "conv_5356"
}
Novaković, J., Tvrzická, E.,& Ražić, S.. (1998). Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography. in Bollettino chimico farmaceutico
Lubner Communication Srl., 137(10), 412-416.
conv_5356
Novaković J, Tvrzická E, Ražić S. Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography. in Bollettino chimico farmaceutico. 1998;137(10):412-416.
conv_5356 .
Novaković, J, Tvrzická, E, Ražić, Slavica, "Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography" in Bollettino chimico farmaceutico, 137, no. 10 (1998):412-416,
conv_5356 .

DSpace software copyright © 2002-2015  DuraSpace
O FarFaR-u | Pošaljite zapažanja

OpenAIRERCUB
 

 

Kompletan repozitorijumInstitucije/grupeAutoriNasloviTemeOva institucijaAutoriNasloviTeme

Statistika

Pregled statistika

DSpace software copyright © 2002-2015  DuraSpace
O FarFaR-u | Pošaljite zapažanja

OpenAIRERCUB