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dc.creatorNovaković, J
dc.creatorTvrzická, E
dc.creatorRažić, Slavica
dc.date.accessioned2019-09-02T10:51:13Z
dc.date.available2019-09-02T10:51:13Z
dc.date.issued1998
dc.identifier.issn0006-6648
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/208
dc.description.abstractHigh-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities.en
dc.publisherLubner Communication Srl
dc.rightsrestrictedAccess
dc.sourceBollettino chimico farmaceutico
dc.subjectFree bile acidsen
dc.subjectHPLC-ELSDen
dc.subjectPharmaceuticalsen
dc.subjectTLC-FIDen
dc.titleDetermination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatographyen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractНоваковић, Ј; Ражић, Славица; Тврзицкá, Е;
dc.citation.volume137
dc.citation.issue10
dc.citation.spage412
dc.citation.epage416
dc.citation.other137(10): 412-416
dc.identifier.scopus2-s2.0-0032435597
dc.identifier.rcubconv_5356
dc.type.versionpublishedVersion


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