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Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography
dc.creator | Novaković, J | |
dc.creator | Tvrzická, E | |
dc.creator | Ražić, Slavica | |
dc.date.accessioned | 2019-09-02T10:51:13Z | |
dc.date.available | 2019-09-02T10:51:13Z | |
dc.date.issued | 1998 | |
dc.identifier.issn | 0006-6648 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/208 | |
dc.description.abstract | High-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and thin layer chromatography with flame ionization detection (TLC-FID) have been applied to the separation of five main free bile acids present in humans : cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA), lithocholic (LCA) and ursodeoxycholic (UDCA) acid. HPLC separation was performed on Biospher Si 100 column using a mixture of n- heptane, isopropanol, ethylacetate, methanol and glacial acetic acid as a mobile phase. All the compounds were separate in less than 12 minutes by using a gradient elution mode. TLC-FID separation was performed on S-II Chromarods with a mixture of isooctane, ethylacetate and glacial acetic acid as a mobile phase. HPLC-ELSD method was applied to the determination of CDCA and UDCA in pharmaceuticals and their purity control when LCA, DCA, and CA were considered as impurities. | en |
dc.publisher | Lubner Communication Srl | |
dc.rights | restrictedAccess | |
dc.source | Bollettino chimico farmaceutico | |
dc.subject | Free bile acids | en |
dc.subject | HPLC-ELSD | en |
dc.subject | Pharmaceuticals | en |
dc.subject | TLC-FID | en |
dc.title | Determination of free bile acids in pharmaceuticals by thin layer cromatography and high performance liquid chromatography | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Новаковић, Ј; Ражић, Славица; Тврзицкá, Е; | |
dc.citation.volume | 137 | |
dc.citation.issue | 10 | |
dc.citation.spage | 412 | |
dc.citation.epage | 416 | |
dc.citation.other | 137(10): 412-416 | |
dc.identifier.scopus | 2-s2.0-0032435597 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_farfar_208 | |
dc.type.version | publishedVersion |
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