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High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-Advantages and limitations
Chromatography techniques such as HPTLC and HPLC are commonly used to produce a chemical fingerprint of a plant to allow identification and quantify the main constituents within the plant. The aims of this study were to compare HPTLC and HPLC, for qualitative and quantitative analysis of the major constituents of Calendula officinalis and to investigate the effect of different extraction techniques on the C officinalis extract composition from different parts of the plant. The results found HPTLC to be effective for qualitative analysis, however, HPLC was found to be more accurate for quantitative analysis. A combination of the two methods may be useful in a quality control setting as it would allow rapid qualitative analysis of herbal material while maintaining accurate quantification of extract composition. (C) 2014 Elsevier B.V. All rights reserved.
TY - JOUR
AU - Loescher, Christine M.
AU - Morton, David W.
AU - Ražić, Slavica
AU - Agatonović-Kuštrin, Snežana
PY - 2014
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2093
AB - Chromatography techniques such as HPTLC and HPLC are commonly used to produce a chemical fingerprint of a plant to allow identification and quantify the main constituents within the plant. The aims of this study were to compare HPTLC and HPLC, for qualitative and quantitative analysis of the major constituents of Calendula officinalis and to investigate the effect of different extraction techniques on the C officinalis extract composition from different parts of the plant. The results found HPTLC to be effective for qualitative analysis, however, HPLC was found to be more accurate for quantitative analysis. A combination of the two methods may be useful in a quality control setting as it would allow rapid qualitative analysis of herbal material while maintaining accurate quantification of extract composition. (C) 2014 Elsevier B.V. All rights reserved.
PB - Elsevier Science BV, Amsterdam
T2 - Journal of Pharmaceutical and Biomedical Analysis
T1 - High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-Advantages and limitations
VL - 98
SP - 52
EP - 59
DO - 10.1016/j.jpba.2014.04.023
ER -
@article{
author = "Loescher, Christine M. and Morton, David W. and Ražić, Slavica and Agatonović-Kuštrin, Snežana",
year = "2014",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/2093",
abstract = "Chromatography techniques such as HPTLC and HPLC are commonly used to produce a chemical fingerprint of a plant to allow identification and quantify the main constituents within the plant. The aims of this study were to compare HPTLC and HPLC, for qualitative and quantitative analysis of the major constituents of Calendula officinalis and to investigate the effect of different extraction techniques on the C officinalis extract composition from different parts of the plant. The results found HPTLC to be effective for qualitative analysis, however, HPLC was found to be more accurate for quantitative analysis. A combination of the two methods may be useful in a quality control setting as it would allow rapid qualitative analysis of herbal material while maintaining accurate quantification of extract composition. (C) 2014 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-Advantages and limitations",
volume = "98",
pages = "52-59",
doi = "10.1016/j.jpba.2014.04.023"
}
Loescher, C. M., Morton, D. W., Ražić, S.,& Agatonović-Kuštrin, S. (2014). High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-Advantages and limitations.
Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 98, 52-59.
https://doi.org/10.1016/j.jpba.2014.04.023
Loescher CM, Morton DW, Ražić S, Agatonović-Kuštrin S. High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-Advantages and limitations. Journal of Pharmaceutical and Biomedical Analysis. 2014;98:52-59
Loescher Christine M., Morton David W., Ražić Slavica, Agatonović-Kuštrin Snežana, "High performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) for the qualitative and quantitative analysis of Calendula officinalis-Advantages and limitations" Journal of Pharmaceutical and Biomedical Analysis, 98 (2014):52-59,
https://doi.org/10.1016/j.jpba.2014.04.023 .
