Mutant p53 protein expression and antioxidant status deficiency in breast cancer
rcub.bitstream.none
Authors
Milicević, ZorkaKasapović, Jelena

Gavrilović, Ljubica

Milovanović, Zorka
Bajić, Vladan

Potparević, Biljana

Article (Published version)
Metadata
Show full item recordAbstract
It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform De...lta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p lt 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p lt 0.001; catalase, CAT, p lt 0.01; glutathione reductase, GR, p lt 0.001; glutathione, GSH, p lt 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p lt 0.05) and lowered antioxidant activity of CAT (- 0.437, p lt 0.01) in the breast cancer patients.
Keywords:
antioxidants / breast carcinoma / lipid peroxidation / p53Source:
EXCLI Journal, 2014, 13, 691-708Publisher:
- Excli Journal Managing Office, Dortmund
Funding / projects:
Collections
Institution/Community
PharmacyTY - JOUR AU - Milicević, Zorka AU - Kasapović, Jelena AU - Gavrilović, Ljubica AU - Milovanović, Zorka AU - Bajić, Vladan AU - Potparević, Biljana PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2096 AB - It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p lt 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p lt 0.001; catalase, CAT, p lt 0.01; glutathione reductase, GR, p lt 0.001; glutathione, GSH, p lt 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p lt 0.05) and lowered antioxidant activity of CAT (- 0.437, p lt 0.01) in the breast cancer patients. PB - Excli Journal Managing Office, Dortmund T2 - EXCLI Journal T1 - Mutant p53 protein expression and antioxidant status deficiency in breast cancer VL - 13 SP - 691 EP - 708 UR - https://hdl.handle.net/21.15107/rcub_vinar_113 ER -
@article{ author = "Milicević, Zorka and Kasapović, Jelena and Gavrilović, Ljubica and Milovanović, Zorka and Bajić, Vladan and Potparević, Biljana", year = "2014", abstract = "It is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p lt 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p lt 0.001; catalase, CAT, p lt 0.01; glutathione reductase, GR, p lt 0.001; glutathione, GSH, p lt 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p lt 0.05) and lowered antioxidant activity of CAT (- 0.437, p lt 0.01) in the breast cancer patients.", publisher = "Excli Journal Managing Office, Dortmund", journal = "EXCLI Journal", title = "Mutant p53 protein expression and antioxidant status deficiency in breast cancer", volume = "13", pages = "691-708", url = "https://hdl.handle.net/21.15107/rcub_vinar_113" }
Milicević, Z., Kasapović, J., Gavrilović, L., Milovanović, Z., Bajić, V.,& Potparević, B.. (2014). Mutant p53 protein expression and antioxidant status deficiency in breast cancer. in EXCLI Journal Excli Journal Managing Office, Dortmund., 13, 691-708. https://hdl.handle.net/21.15107/rcub_vinar_113
Milicević Z, Kasapović J, Gavrilović L, Milovanović Z, Bajić V, Potparević B. Mutant p53 protein expression and antioxidant status deficiency in breast cancer. in EXCLI Journal. 2014;13:691-708. https://hdl.handle.net/21.15107/rcub_vinar_113 .
Milicević, Zorka, Kasapović, Jelena, Gavrilović, Ljubica, Milovanović, Zorka, Bajić, Vladan, Potparević, Biljana, "Mutant p53 protein expression and antioxidant status deficiency in breast cancer" in EXCLI Journal, 13 (2014):691-708, https://hdl.handle.net/21.15107/rcub_vinar_113 .