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dc.creatorMilicević, Zorka
dc.creatorKasapović, Jelena
dc.creatorGavrilović, Ljubica
dc.creatorMilovanović, Zorka
dc.creatorBajić, Vladan
dc.creatorPotparević, Biljana
dc.date.accessioned2019-09-02T11:38:55Z
dc.date.available2019-09-02T11:38:55Z
dc.date.issued2014
dc.identifier.issn1611-2156
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2096
dc.description.abstractIt is well recognized that cancers develop and grow as a result of disordered function of tumor suppressor genes and oncogenes, which may be exploited for screening purposes. Extensive evidence indicated tumor suppressor protein p53 as candidate marker for mutation identification. We have investigated mutant p53 protein expression in human breast tumors in relation to antioxidant status deficiency. The study included 100 breast cancer patients. p53 protein expression was evaluated by Western blot assay and immunostaining using a CM-1, DO-7 and Pab240 antibodies. Antioxidant parameters and lipid peroxidation were estimated by biochemical analyses. Western blotting with epitope-specific monoclonal antibody Pab240 strongly suggests that nuclear extracts from breast cancer cells express mutant forms of p53. It is of interest that the mutant forms of p53 overexpression in conjunction with the appearance of nuclear bodies are observed in highly aggressive carcinomas. Expression of isoform Delta p53 (45 kDa) and isoform of similar to 29 kDa were more common in cases with LN metastasis. These studies point out the molecular consequences of oxidative stress (lipid peroxides, LP, p lt 0.001) and antioxidant status deficiency (copper, zinc superoxid dismutase, SOD, p lt 0.001; catalase, CAT, p lt 0.01; glutathione reductase, GR, p lt 0.001; glutathione, GSH, p lt 0.05) and indicate the importance of p53 mutation as the commonest genetic alteration detected in breast cancer cells. The expression of mutant p53 is correlated to increased lipid peroxides (0.346, p lt 0.05) and lowered antioxidant activity of CAT (- 0.437, p lt 0.01) in the breast cancer patients.en
dc.publisherExcli Journal Managing Office, Dortmund
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173034/RS//
dc.rightsopenAccess
dc.sourceEXCLI Journal
dc.subjectantioxidantsen
dc.subjectbreast carcinomaen
dc.subjectlipid peroxidationen
dc.subjectp53en
dc.titleMutant p53 protein expression and antioxidant status deficiency in breast canceren
dc.typearticle
dc.rights.licenseBY
dcterms.abstractГавриловић, Љубица; Касаповић, Јелена; Потпаревић, Биљана; Бајић, Владан; Милицевић, Зорка; Миловановић, Зорка;
dc.citation.volume13
dc.citation.spage691
dc.citation.epage708
dc.citation.other13: 691-708
dc.citation.rankM23
dc.identifier.wos000341518200016
dc.identifier.pmid26417293
dc.identifier.rcubconv_3153
dc.type.versionpublishedVersion


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