Effects of short- and long-term physical activity on DNA stability and oxidative stress status in young soccer players
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Аутори
Sopić, MironBogavac-Stanojević, Nataša
Baralić, Ivana
Kotur-Stevuljević, Jelena
Đorđević, Brižita
Stefanović, Aleksandra
Jelić-Ivanović, Zorana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aim. The aim of the study was to determine whether single soccer training is able to cause oxidative stress and DNA instability. We have also sought to investigate if adaptive response will be developed during 45 days training period and to what extent. Methods. This study was conducted on 16 soccer players aged 18.13 +/- 0.35 years. We used single cells gel electrophoresis (comet assay) to investigate leukocyte DNA stability. The results were presented as DNA score and percent of cells with medium and high damage. Oxidative status of our subjects was estimated through blood levels of superoxide anion, the thiobarbituric acid-reacting substances (TBARs), total antioxidant status (TAS), total oxidant status (TOS), prooxidant-antioxidant balance (PAB) and sulfhydryl-groups (SH-groups). Results. During single soccer training, DNA score and percent of the cells with medium and high damage were increased after training but without significance. Sulphydryl-groups (P=0.033), TOS (P=0.002) and... PAB (P=0.045) were significantly lower after training. After 45 days training period DNA score was decreased but with no significance. However, percent of cells with medium and high damage was significantly lower (P=0.01). TOS (P=0.001) and MDA (P=0.038) levels were also significantly lower, while sulphydryl-groups levels were significantly higher (P=0.006). Conclusion. This study demonstrated that single soccer training had not compromised DNA stability. Possible development of oxidative stress was effectively neutralized by very well preserved antioxidative mechanisms. It was also shown that during 45 days adaptive response was induced. All measured parameters should be considered as useful information on oxidative status of trainees.
Кључне речи:
DNA / Soccer / Oxidative stressИзвор:
Journal of Sports Medicine and Physical Fitness, 2014, 54, 3, 354-361Издавач:
- Edizioni Minerva Medica, Turin
Финансирање / пројекти:
Институција/група
PharmacyTY - JOUR AU - Sopić, Miron AU - Bogavac-Stanojević, Nataša AU - Baralić, Ivana AU - Kotur-Stevuljević, Jelena AU - Đorđević, Brižita AU - Stefanović, Aleksandra AU - Jelić-Ivanović, Zorana PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2115 AB - Aim. The aim of the study was to determine whether single soccer training is able to cause oxidative stress and DNA instability. We have also sought to investigate if adaptive response will be developed during 45 days training period and to what extent. Methods. This study was conducted on 16 soccer players aged 18.13 +/- 0.35 years. We used single cells gel electrophoresis (comet assay) to investigate leukocyte DNA stability. The results were presented as DNA score and percent of cells with medium and high damage. Oxidative status of our subjects was estimated through blood levels of superoxide anion, the thiobarbituric acid-reacting substances (TBARs), total antioxidant status (TAS), total oxidant status (TOS), prooxidant-antioxidant balance (PAB) and sulfhydryl-groups (SH-groups). Results. During single soccer training, DNA score and percent of the cells with medium and high damage were increased after training but without significance. Sulphydryl-groups (P=0.033), TOS (P=0.002) and PAB (P=0.045) were significantly lower after training. After 45 days training period DNA score was decreased but with no significance. However, percent of cells with medium and high damage was significantly lower (P=0.01). TOS (P=0.001) and MDA (P=0.038) levels were also significantly lower, while sulphydryl-groups levels were significantly higher (P=0.006). Conclusion. This study demonstrated that single soccer training had not compromised DNA stability. Possible development of oxidative stress was effectively neutralized by very well preserved antioxidative mechanisms. It was also shown that during 45 days adaptive response was induced. All measured parameters should be considered as useful information on oxidative status of trainees. PB - Edizioni Minerva Medica, Turin T2 - Journal of Sports Medicine and Physical Fitness T1 - Effects of short- and long-term physical activity on DNA stability and oxidative stress status in young soccer players VL - 54 IS - 3 SP - 354 EP - 361 UR - https://hdl.handle.net/21.15107/rcub_farfar_2115 ER -
@article{ author = "Sopić, Miron and Bogavac-Stanojević, Nataša and Baralić, Ivana and Kotur-Stevuljević, Jelena and Đorđević, Brižita and Stefanović, Aleksandra and Jelić-Ivanović, Zorana", year = "2014", abstract = "Aim. The aim of the study was to determine whether single soccer training is able to cause oxidative stress and DNA instability. We have also sought to investigate if adaptive response will be developed during 45 days training period and to what extent. Methods. This study was conducted on 16 soccer players aged 18.13 +/- 0.35 years. We used single cells gel electrophoresis (comet assay) to investigate leukocyte DNA stability. The results were presented as DNA score and percent of cells with medium and high damage. Oxidative status of our subjects was estimated through blood levels of superoxide anion, the thiobarbituric acid-reacting substances (TBARs), total antioxidant status (TAS), total oxidant status (TOS), prooxidant-antioxidant balance (PAB) and sulfhydryl-groups (SH-groups). Results. During single soccer training, DNA score and percent of the cells with medium and high damage were increased after training but without significance. Sulphydryl-groups (P=0.033), TOS (P=0.002) and PAB (P=0.045) were significantly lower after training. After 45 days training period DNA score was decreased but with no significance. However, percent of cells with medium and high damage was significantly lower (P=0.01). TOS (P=0.001) and MDA (P=0.038) levels were also significantly lower, while sulphydryl-groups levels were significantly higher (P=0.006). Conclusion. This study demonstrated that single soccer training had not compromised DNA stability. Possible development of oxidative stress was effectively neutralized by very well preserved antioxidative mechanisms. It was also shown that during 45 days adaptive response was induced. All measured parameters should be considered as useful information on oxidative status of trainees.", publisher = "Edizioni Minerva Medica, Turin", journal = "Journal of Sports Medicine and Physical Fitness", title = "Effects of short- and long-term physical activity on DNA stability and oxidative stress status in young soccer players", volume = "54", number = "3", pages = "354-361", url = "https://hdl.handle.net/21.15107/rcub_farfar_2115" }
Sopić, M., Bogavac-Stanojević, N., Baralić, I., Kotur-Stevuljević, J., Đorđević, B., Stefanović, A.,& Jelić-Ivanović, Z.. (2014). Effects of short- and long-term physical activity on DNA stability and oxidative stress status in young soccer players. in Journal of Sports Medicine and Physical Fitness Edizioni Minerva Medica, Turin., 54(3), 354-361. https://hdl.handle.net/21.15107/rcub_farfar_2115
Sopić M, Bogavac-Stanojević N, Baralić I, Kotur-Stevuljević J, Đorđević B, Stefanović A, Jelić-Ivanović Z. Effects of short- and long-term physical activity on DNA stability and oxidative stress status in young soccer players. in Journal of Sports Medicine and Physical Fitness. 2014;54(3):354-361. https://hdl.handle.net/21.15107/rcub_farfar_2115 .
Sopić, Miron, Bogavac-Stanojević, Nataša, Baralić, Ivana, Kotur-Stevuljević, Jelena, Đorđević, Brižita, Stefanović, Aleksandra, Jelić-Ivanović, Zorana, "Effects of short- and long-term physical activity on DNA stability and oxidative stress status in young soccer players" in Journal of Sports Medicine and Physical Fitness, 54, no. 3 (2014):354-361, https://hdl.handle.net/21.15107/rcub_farfar_2115 .