Cadmium and decabrominated diphenyl ether mixture: In vitro evaluation of cytotoxic, prooxidative and genotoxic effects
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2014
Authors
Ćurčić, Marijana
Durgo, Ksenija
Kopjar, Nevenka
Ancić, Mario
Vučinić, Slavica

Antonijević, Biljana

Article (Published version)

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In order to look into the combined effects of Cd and BDE-209 in vitro, this study was aimed at examining cytotoxic and genotoxic effects using the human colon carcinoma cell line (SW 480) as a biological test system as well as to determine if ROS production was one of the possible mechanisms of their mixture action. This cell line was chosen since ingestion of contaminated food/water represents an important route of exposure to both Cd and BDE-209, which is why intestinal cells are a common target for the contaminants present in food and water. Cells were treated with single Cd in concentrations of 2.5, 7.5 or 15 mu g Cd/mL (corresponding to 22, 67 or 134 mu M), single BDE-209 in concentrations of 2.5, 5 or 10 mu g BDE209/mL (corresponding to 2.5, 5 or 10 mu M), and their mixtures (design 3 x 3). Mixture of Cd and BDE-209 has shown clear potential to reduce the viability of SW 480 cells, as evidenced by cytotoxicity associated with ROS generation. Factorial regression models used to id...entify type of interaction revealed synergism related to mixture citotoxicity and additive interaction for the effect on ROS production. The results from this introductory study could contribute to the issue of possible adverse effects associated with co-exposure and body burden with two persistent environmental pollutants, Cd and BDE-209.
Keywords:
Cd / BDE-209 / Mixture / cyto-/genotoxicity / ROS / InteractionsSource:
Environmental Toxicology and Pharmacology, 2014, 38, 2, 663-671Publisher:
- Elsevier Science BV, Amsterdam
Funding / projects:
DOI: 10.1016/j.etap.2014.07.021
ISSN: 1382-6689
PubMed: 25218094
WoS: 000343838300035
Scopus: 2-s2.0-84907995708
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PharmacyTY - JOUR AU - Ćurčić, Marijana AU - Durgo, Ksenija AU - Kopjar, Nevenka AU - Ancić, Mario AU - Vučinić, Slavica AU - Antonijević, Biljana PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2128 AB - In order to look into the combined effects of Cd and BDE-209 in vitro, this study was aimed at examining cytotoxic and genotoxic effects using the human colon carcinoma cell line (SW 480) as a biological test system as well as to determine if ROS production was one of the possible mechanisms of their mixture action. This cell line was chosen since ingestion of contaminated food/water represents an important route of exposure to both Cd and BDE-209, which is why intestinal cells are a common target for the contaminants present in food and water. Cells were treated with single Cd in concentrations of 2.5, 7.5 or 15 mu g Cd/mL (corresponding to 22, 67 or 134 mu M), single BDE-209 in concentrations of 2.5, 5 or 10 mu g BDE209/mL (corresponding to 2.5, 5 or 10 mu M), and their mixtures (design 3 x 3). Mixture of Cd and BDE-209 has shown clear potential to reduce the viability of SW 480 cells, as evidenced by cytotoxicity associated with ROS generation. Factorial regression models used to identify type of interaction revealed synergism related to mixture citotoxicity and additive interaction for the effect on ROS production. The results from this introductory study could contribute to the issue of possible adverse effects associated with co-exposure and body burden with two persistent environmental pollutants, Cd and BDE-209. PB - Elsevier Science BV, Amsterdam T2 - Environmental Toxicology and Pharmacology T1 - Cadmium and decabrominated diphenyl ether mixture: In vitro evaluation of cytotoxic, prooxidative and genotoxic effects VL - 38 IS - 2 SP - 663 EP - 671 DO - 10.1016/j.etap.2014.07.021 ER -
@article{ author = "Ćurčić, Marijana and Durgo, Ksenija and Kopjar, Nevenka and Ancić, Mario and Vučinić, Slavica and Antonijević, Biljana", year = "2014", abstract = "In order to look into the combined effects of Cd and BDE-209 in vitro, this study was aimed at examining cytotoxic and genotoxic effects using the human colon carcinoma cell line (SW 480) as a biological test system as well as to determine if ROS production was one of the possible mechanisms of their mixture action. This cell line was chosen since ingestion of contaminated food/water represents an important route of exposure to both Cd and BDE-209, which is why intestinal cells are a common target for the contaminants present in food and water. Cells were treated with single Cd in concentrations of 2.5, 7.5 or 15 mu g Cd/mL (corresponding to 22, 67 or 134 mu M), single BDE-209 in concentrations of 2.5, 5 or 10 mu g BDE209/mL (corresponding to 2.5, 5 or 10 mu M), and their mixtures (design 3 x 3). Mixture of Cd and BDE-209 has shown clear potential to reduce the viability of SW 480 cells, as evidenced by cytotoxicity associated with ROS generation. Factorial regression models used to identify type of interaction revealed synergism related to mixture citotoxicity and additive interaction for the effect on ROS production. The results from this introductory study could contribute to the issue of possible adverse effects associated with co-exposure and body burden with two persistent environmental pollutants, Cd and BDE-209.", publisher = "Elsevier Science BV, Amsterdam", journal = "Environmental Toxicology and Pharmacology", title = "Cadmium and decabrominated diphenyl ether mixture: In vitro evaluation of cytotoxic, prooxidative and genotoxic effects", volume = "38", number = "2", pages = "663-671", doi = "10.1016/j.etap.2014.07.021" }
Ćurčić, M., Durgo, K., Kopjar, N., Ancić, M., Vučinić, S.,& Antonijević, B.. (2014). Cadmium and decabrominated diphenyl ether mixture: In vitro evaluation of cytotoxic, prooxidative and genotoxic effects. in Environmental Toxicology and Pharmacology Elsevier Science BV, Amsterdam., 38(2), 663-671. https://doi.org/10.1016/j.etap.2014.07.021
Ćurčić M, Durgo K, Kopjar N, Ancić M, Vučinić S, Antonijević B. Cadmium and decabrominated diphenyl ether mixture: In vitro evaluation of cytotoxic, prooxidative and genotoxic effects. in Environmental Toxicology and Pharmacology. 2014;38(2):663-671. doi:10.1016/j.etap.2014.07.021 .
Ćurčić, Marijana, Durgo, Ksenija, Kopjar, Nevenka, Ancić, Mario, Vučinić, Slavica, Antonijević, Biljana, "Cadmium and decabrominated diphenyl ether mixture: In vitro evaluation of cytotoxic, prooxidative and genotoxic effects" in Environmental Toxicology and Pharmacology, 38, no. 2 (2014):663-671, https://doi.org/10.1016/j.etap.2014.07.021 . .