Show simple item record

dc.creatorSrecković, Ivana
dc.creatorBirner-Gruenberger, Ruth
dc.creatorBesenboeck, Carolin
dc.creatorMiljković, Milica
dc.creatorStojaković, Tatjana
dc.creatorScharnagl, Hubert
dc.creatorMarsche, Gunther
dc.creatorLang, Uwe
dc.creatorKotur-Stevuljević, Jelena
dc.creatorJelić-Ivanović, Zorana
dc.creatorDesoye, Gernot
dc.creatorWadsack, Christian
dc.date.accessioned2019-09-02T11:39:46Z
dc.date.available2019-09-02T11:39:46Z
dc.date.issued2014
dc.identifier.issn1388-1981
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2129
dc.description.abstractGestational diabetes mellitus (GDM) is related to neonatal macrosomia and an increased risk of vascular events. We hypothesized that GDM exerts qualitative effects on neonatal high-density lipoprotein (HDL). HDL was isolated from control (n = 11) and GDM maternal/neonatal donors (n = 9) and subjected to shotgun proteomics. Differences in HDL mobility were assessed by FPLC and native gel-electrophoresis. Paraoxonase (PON1) activity, cholesterol ester-transfer protein (CETP) mass and activity, phospholipid, triglyceride and cholesterol concentrations were quantified with commercial kits. Total anti-oxidative capacity and cholesterol efflux capability of HDLs were measured. Four proteins involved in lipid metabolism, inflammation and innate immunity were differentially expressed between controls and GDM neonates. ApoM (decreased, p lt 0.05) and SAA1 (increased, p lt 0.05) showed the same differences on both, maternal and neonatal GDM HDL Lower PON1 protein expression was corroborated by lower activity (p lt 0.05) which in turn was associated with attenuated anti-oxidant capacity of GDM HDL Protein changes were accompanied by increased levels of triglycerides and decreased levels of cholesterol esters, respectively. The observed differences in GDM HDL lipid moiety may be related to CETP mass and activity alterations. The rate of cholesterol efflux from term trophoblasts to maternal and from placental endothelial cells to neonatal GDM HDL was impaired (p lt 0.05). In conclusion, GDM causes changes in HDL composition and is intimately associated with impaired cholesterol efflux capability as well as diminished anti-oxidative particle properties. Remodeling of neonatal GDM HDL in utero supports the hypothesis that maternal conditions in pregnancy impact neonatal lipoprotein metabolism.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationPhD Program Molecular Medicine of the Medical University of Graz
dc.rightsrestrictedAccess
dc.sourceBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
dc.subjectNeonatal HDLen
dc.subjectGDMen
dc.subjectAnti-oxidative propertyen
dc.titleGestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneityen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКотур-Стевуљевић, Јелена; Јелић-Ивановић, Зорана; Бирнер-Груенбергер, Рутх; Стојаковић, Татјана; Сцхарнагл, Хуберт; Ланг, Уwе; Десоyе, Гернот; Wадсацк, Цхристиан; Срецковић, Ивана; Бесенбоецк, Царолин; Марсцхе, Гунтхер; Миљковић, Милица;
dc.citation.volume1841
dc.citation.issue11
dc.citation.spage1619
dc.citation.epage1627
dc.citation.other1841(11): 1619-1627
dc.citation.rankM21
dc.identifier.wos000343854300006
dc.identifier.doi10.1016/j.bbalip.2014.07.021
dc.identifier.pmid25130684
dc.identifier.scopus2-s2.0-84907720790
dc.identifier.rcubconv_3185
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record