FarFaR - Pharmacy Repository
University of Belgrade, Faculty of Pharmacy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrilic)
    • Serbian (Latin)
  • Login
View Item 
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions

Thumbnail
2014
2136.pdf (293.3Kb)
Authors
Aleksić, Mara
Kapetanović, Vera
Article (Published version)
Metadata
Show full item record
Abstract
A large number of inorganic and organic compounds is able to bind to DNA and form complexes. Among them, drugs are very important, especially chemotherapeutics. This paper presents the overview of DNA structural characteristics and types of interactions (covalent and non-covalent) between DNA molecule and drugs. Covalent binding of the drug is irreversible and leads to complete inhibition of DNA function, what conclusively, causes the cell death. On the other hand, non-covalent binding is reversible and based on the principle of molecular recognition. Special attention is given to elucidation of the specific sites in DNA molecule for drug binding. According to their structural characteristics, drugs that react non-covalently with DNA are mainly intercalators, but also minor and major groove binders. When the complex between drug and DNA is formed, both the drug molecule, as well as DNA, experienced some modifications. This paper presents the overview of the methods used for the study o...f the interactions between DNA and drugs with the aim of detection and explanation of the resulting changes. For this purpose many spectroscopic methods like UV/VIS, fluorescence, infrared and NMR, polarized light spectroscopies like circular and linear dichroism, and fluorescence anisotropy or resonance is used. The development of the electrochemical DNA biosensors has opened a wide perspective using particularly sensitive and selective electrochemical methods for the detection of specific DNA interactions. The presented results summarize literature data obtained by the mentioned methods. The results are used to confirm the DNA damage, to determine drug binding sites and sequence preference, as well as conformational changes due to drug-DNA interaction.

Keywords:
DNA / drug / interaction / spectroscopic methods / electrochemical DNA-biosensors
Source:
Acta Chimica Slovenica, 2014, 61, 3, 555-573
Publisher:
  • Slovensko Kemijsko Drustvo, Ljubljana
Projects:
  • Synthesis, Quantitative Structure and Activity Relationship, Physico-Chemical Characterisation and Analysis of Pharmacologically Active Substances (RS-172033)

ISSN: 1318-0207

PubMed: 25286211

WoS: 000345283300018

Scopus: 2-s2.0-84907184344
[ Google Scholar ]
47
48
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2138
Collections
  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy
TY  - JOUR
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
PY  - 2014
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2138
AB  - A large number of inorganic and organic compounds is able to bind to DNA and form complexes. Among them, drugs are very important, especially chemotherapeutics. This paper presents the overview of DNA structural characteristics and types of interactions (covalent and non-covalent) between DNA molecule and drugs. Covalent binding of the drug is irreversible and leads to complete inhibition of DNA function, what conclusively, causes the cell death. On the other hand, non-covalent binding is reversible and based on the principle of molecular recognition. Special attention is given to elucidation of the specific sites in DNA molecule for drug binding. According to their structural characteristics, drugs that react non-covalently with DNA are mainly intercalators, but also minor and major groove binders. When the complex between drug and DNA is formed, both the drug molecule, as well as DNA, experienced some modifications. This paper presents the overview of the methods used for the study of the interactions between DNA and drugs with the aim of detection and explanation of the resulting changes. For this purpose many spectroscopic methods like UV/VIS, fluorescence, infrared and NMR, polarized light spectroscopies like circular and linear dichroism, and fluorescence anisotropy or resonance is used. The development of the electrochemical DNA biosensors has opened a wide perspective using particularly sensitive and selective electrochemical methods for the detection of specific DNA interactions. The presented results summarize literature data obtained by the mentioned methods. The results are used to confirm the DNA damage, to determine drug binding sites and sequence preference, as well as conformational changes due to drug-DNA interaction.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions
VL  - 61
IS  - 3
SP  - 555
EP  - 573
ER  - 
@article{
author = "Aleksić, Mara and Kapetanović, Vera",
year = "2014",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/2138",
abstract = "A large number of inorganic and organic compounds is able to bind to DNA and form complexes. Among them, drugs are very important, especially chemotherapeutics. This paper presents the overview of DNA structural characteristics and types of interactions (covalent and non-covalent) between DNA molecule and drugs. Covalent binding of the drug is irreversible and leads to complete inhibition of DNA function, what conclusively, causes the cell death. On the other hand, non-covalent binding is reversible and based on the principle of molecular recognition. Special attention is given to elucidation of the specific sites in DNA molecule for drug binding. According to their structural characteristics, drugs that react non-covalently with DNA are mainly intercalators, but also minor and major groove binders. When the complex between drug and DNA is formed, both the drug molecule, as well as DNA, experienced some modifications. This paper presents the overview of the methods used for the study of the interactions between DNA and drugs with the aim of detection and explanation of the resulting changes. For this purpose many spectroscopic methods like UV/VIS, fluorescence, infrared and NMR, polarized light spectroscopies like circular and linear dichroism, and fluorescence anisotropy or resonance is used. The development of the electrochemical DNA biosensors has opened a wide perspective using particularly sensitive and selective electrochemical methods for the detection of specific DNA interactions. The presented results summarize literature data obtained by the mentioned methods. The results are used to confirm the DNA damage, to determine drug binding sites and sequence preference, as well as conformational changes due to drug-DNA interaction.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions",
volume = "61",
number = "3",
pages = "555-573"
}
Aleksić M, Kapetanović V. An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions. Acta Chimica Slovenica. 2014;61(3):555-573
Aleksić, M.,& Kapetanović, V. (2014). An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions.
Acta Chimica SlovenicaSlovensko Kemijsko Drustvo, Ljubljana., 61(3), 555-573.
Aleksić Mara, Kapetanović Vera, "An Overview of the Optical and Electrochemical Methods for Detection of DNA - Drug Interactions" 61, no. 3 (2014):555-573

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutionsAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB