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Quantitative analysis of sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate in pharmaceuticals by high-performance thin-layer chromatography and scanning densitometry
A simple and reliable thin-layer chromatographic method for determining sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate was developed and validated. A methylene chloride-methanol-ammonia solution (25%; 18 + 2.8 + 0.4, v/v) solvent system is used for separation and quantitative evaluation of chromatograms. The chromatographic plate is first scanned at 240 nm to locate chromatographic zones corresponding to sulpiride and methyl-5-sulphamoyl-2-methoxybenzoate. Then 2-aminomethyl-1-ethylpyrrolidine is derivatized in situ with ninhydrin, and resulting colored spots are measured at 500 nm. The method is reproducible and convenient for quantitative analysis and purity control of sulpiride in its raw material and in its dosage forms.
Source:
Journal of AOAC International, 1999, 82, 4, 825-829
TY - JOUR
AU - Agbaba, Danica
AU - Miljković, Tatjana
AU - Marinković, Valentina
AU - Živanov-Stakić, Dobrila
AU - Vladimirov, Sote
PY - 1999
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/214
AB - A simple and reliable thin-layer chromatographic method for determining sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate was developed and validated. A methylene chloride-methanol-ammonia solution (25%; 18 + 2.8 + 0.4, v/v) solvent system is used for separation and quantitative evaluation of chromatograms. The chromatographic plate is first scanned at 240 nm to locate chromatographic zones corresponding to sulpiride and methyl-5-sulphamoyl-2-methoxybenzoate. Then 2-aminomethyl-1-ethylpyrrolidine is derivatized in situ with ninhydrin, and resulting colored spots are measured at 500 nm. The method is reproducible and convenient for quantitative analysis and purity control of sulpiride in its raw material and in its dosage forms.
PB - AOAC International, Gaithersburg
T2 - Journal of AOAC International
T1 - Quantitative analysis of sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate in pharmaceuticals by high-performance thin-layer chromatography and scanning densitometry
VL - 82
IS - 4
SP - 825
EP - 829
DO - 10.1093/jaoac/82.4.825
ER -
@article{
author = "Agbaba, Danica and Miljković, Tatjana and Marinković, Valentina and Živanov-Stakić, Dobrila and Vladimirov, Sote",
year = "1999",
abstract = "A simple and reliable thin-layer chromatographic method for determining sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate was developed and validated. A methylene chloride-methanol-ammonia solution (25%; 18 + 2.8 + 0.4, v/v) solvent system is used for separation and quantitative evaluation of chromatograms. The chromatographic plate is first scanned at 240 nm to locate chromatographic zones corresponding to sulpiride and methyl-5-sulphamoyl-2-methoxybenzoate. Then 2-aminomethyl-1-ethylpyrrolidine is derivatized in situ with ninhydrin, and resulting colored spots are measured at 500 nm. The method is reproducible and convenient for quantitative analysis and purity control of sulpiride in its raw material and in its dosage forms.",
publisher = "AOAC International, Gaithersburg",
journal = "Journal of AOAC International",
title = "Quantitative analysis of sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate in pharmaceuticals by high-performance thin-layer chromatography and scanning densitometry",
volume = "82",
number = "4",
pages = "825-829",
doi = "10.1093/jaoac/82.4.825"
}
Agbaba, D., Miljković, T., Marinković, V., Živanov-Stakić, D.,& Vladimirov, S.. (1999). Quantitative analysis of sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate in pharmaceuticals by high-performance thin-layer chromatography and scanning densitometry. in Journal of AOAC International
AOAC International, Gaithersburg., 82(4), 825-829.
https://doi.org/10.1093/jaoac/82.4.825
Agbaba D, Miljković T, Marinković V, Živanov-Stakić D, Vladimirov S. Quantitative analysis of sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate in pharmaceuticals by high-performance thin-layer chromatography and scanning densitometry. in Journal of AOAC International. 1999;82(4):825-829.
doi:10.1093/jaoac/82.4.825 .
Agbaba, Danica, Miljković, Tatjana, Marinković, Valentina, Živanov-Stakić, Dobrila, Vladimirov, Sote, "Quantitative analysis of sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate in pharmaceuticals by high-performance thin-layer chromatography and scanning densitometry" in Journal of AOAC International, 82, no. 4 (1999):825-829,
https://doi.org/10.1093/jaoac/82.4.825 . .
