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Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease

Authorized Users Only
2014
Authors
Stanković, Milena N.
Mladenović, Dušan
Đuričić, Ivana
Šobajić, Slađana
Timić, Jasmina
Jorgačević, Bojan
Aleksić, Vuk
Vucević, Danijela B.
Jesić-Vukicević, Rada
Radosavljević, Tatjana S.
Article (Published version)
Metadata
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Abstract
Background and Aims. Methionine-choline deficient (MCD) diet duration necessary for development of non-alcoholic fatty liver disease (NAFLD) and the dynamic of lipid profile and fatty acids are not completely established. The study examined dynamics and association between liver free fatty acids (FFA), serum lipid profile and liver morphological changes on MCD diet-induced NAFLD in mice. Methods. Male C57BL/6 mice (n = 28) were divided into four groups (n = 7 per group): control: fed with standard chow, MCD diet-fed groups: 2, 4 or 6 weeks. After treatment, liver and blood samples were taken for histopathology, serum lipid profile, and liver FFA composition. Results. Hepatic FFA profile showed a decrease in saturated acids, arachidonic and docosahexaenoic acid, whereas proportions of docosapentaenoic, oleic and linoleic acid were increased. Total cholesterol, HDL and triglycerides progressively decreased, whereas LDL level progressively increased. Focal fatty change in the liver appear...ed after 2 weeks, whereas diffuse fatty change with severe inflammation and ballooned hepatocytes were evident after 6 weeks. Conclusions. Six-week diet model may be appropriate for investigation of the role of lipotoxicity in the progression of NAFLD. Therefore, supplementation with n-3 polyunsaturated acid like DHA, rather than DPA, especially in the initial stage of fatty liver disease, may potentially have preventive effects and alleviate development of NAFLD/NASH and may also potentially reduce cardiovascular risk by moderating dyslipidemia.

Keywords:
Free fatty acids / Lipid status / Methionine-choline deficient diet / NAFLD / Mice
Source:
Archives of Medical Research, 2014, 45, 2, 116-124
Publisher:
  • Elsevier Science Inc, New York
Projects:
  • The role of neuroendocrine-inflammatory axis in the pathogenesis of non-alcoholic fatty liver disease (RS-175015)

DOI: 10.1016/j.arcmed.2013.12.010

ISSN: 0188-4409

PubMed: 24480733

WoS: 000333139100002

Scopus: 2-s2.0-84896879418
[ Google Scholar ]
36
35
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2153
Collections
  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy
TY  - JOUR
AU  - Stanković, Milena N.
AU  - Mladenović, Dušan
AU  - Đuričić, Ivana
AU  - Šobajić, Slađana
AU  - Timić, Jasmina
AU  - Jorgačević, Bojan
AU  - Aleksić, Vuk
AU  - Vucević, Danijela B.
AU  - Jesić-Vukicević, Rada
AU  - Radosavljević, Tatjana S.
PY  - 2014
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2153
AB  - Background and Aims. Methionine-choline deficient (MCD) diet duration necessary for development of non-alcoholic fatty liver disease (NAFLD) and the dynamic of lipid profile and fatty acids are not completely established. The study examined dynamics and association between liver free fatty acids (FFA), serum lipid profile and liver morphological changes on MCD diet-induced NAFLD in mice. Methods. Male C57BL/6 mice (n = 28) were divided into four groups (n = 7 per group): control: fed with standard chow, MCD diet-fed groups: 2, 4 or 6 weeks. After treatment, liver and blood samples were taken for histopathology, serum lipid profile, and liver FFA composition. Results. Hepatic FFA profile showed a decrease in saturated acids, arachidonic and docosahexaenoic acid, whereas proportions of docosapentaenoic, oleic and linoleic acid were increased. Total cholesterol, HDL and triglycerides progressively decreased, whereas LDL level progressively increased. Focal fatty change in the liver appeared after 2 weeks, whereas diffuse fatty change with severe inflammation and ballooned hepatocytes were evident after 6 weeks. Conclusions. Six-week diet model may be appropriate for investigation of the role of lipotoxicity in the progression of NAFLD. Therefore, supplementation with n-3 polyunsaturated acid like DHA, rather than DPA, especially in the initial stage of fatty liver disease, may potentially have preventive effects and alleviate development of NAFLD/NASH and may also potentially reduce cardiovascular risk by moderating dyslipidemia.
PB  - Elsevier Science Inc, New York
T2  - Archives of Medical Research
T1  - Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease
VL  - 45
IS  - 2
SP  - 116
EP  - 124
DO  - 10.1016/j.arcmed.2013.12.010
ER  - 
@article{
author = "Stanković, Milena N. and Mladenović, Dušan and Đuričić, Ivana and Šobajić, Slađana and Timić, Jasmina and Jorgačević, Bojan and Aleksić, Vuk and Vucević, Danijela B. and Jesić-Vukicević, Rada and Radosavljević, Tatjana S.",
year = "2014",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/2153",
abstract = "Background and Aims. Methionine-choline deficient (MCD) diet duration necessary for development of non-alcoholic fatty liver disease (NAFLD) and the dynamic of lipid profile and fatty acids are not completely established. The study examined dynamics and association between liver free fatty acids (FFA), serum lipid profile and liver morphological changes on MCD diet-induced NAFLD in mice. Methods. Male C57BL/6 mice (n = 28) were divided into four groups (n = 7 per group): control: fed with standard chow, MCD diet-fed groups: 2, 4 or 6 weeks. After treatment, liver and blood samples were taken for histopathology, serum lipid profile, and liver FFA composition. Results. Hepatic FFA profile showed a decrease in saturated acids, arachidonic and docosahexaenoic acid, whereas proportions of docosapentaenoic, oleic and linoleic acid were increased. Total cholesterol, HDL and triglycerides progressively decreased, whereas LDL level progressively increased. Focal fatty change in the liver appeared after 2 weeks, whereas diffuse fatty change with severe inflammation and ballooned hepatocytes were evident after 6 weeks. Conclusions. Six-week diet model may be appropriate for investigation of the role of lipotoxicity in the progression of NAFLD. Therefore, supplementation with n-3 polyunsaturated acid like DHA, rather than DPA, especially in the initial stage of fatty liver disease, may potentially have preventive effects and alleviate development of NAFLD/NASH and may also potentially reduce cardiovascular risk by moderating dyslipidemia.",
publisher = "Elsevier Science Inc, New York",
journal = "Archives of Medical Research",
title = "Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease",
volume = "45",
number = "2",
pages = "116-124",
doi = "10.1016/j.arcmed.2013.12.010"
}
Stanković MN, Mladenović D, Đuričić I, Šobajić S, Timić J, Jorgačević B, Aleksić V, Vucević DB, Jesić-Vukicević R, Radosavljević TS. Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease. Archives of Medical Research. 2014;45(2):116-124
Stanković, M. N., Mladenović, D., Đuričić, I., Šobajić, S., Timić, J., Jorgačević, B., Aleksić, V., Vucević, D. B., Jesić-Vukicević, R.,& Radosavljević, T. S. (2014). Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease.
Archives of Medical ResearchElsevier Science Inc, New York., 45(2), 116-124.
https://doi.org/10.1016/j.arcmed.2013.12.010
Stanković Milena N., Mladenović Dušan, Đuričić Ivana, Šobajić Slađana, Timić Jasmina, Jorgačević Bojan, Aleksić Vuk, Vucević Danijela B., Jesić-Vukicević Rada, Radosavljević Tatjana S., "Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease" 45, no. 2 (2014):116-124,
https://doi.org/10.1016/j.arcmed.2013.12.010 .

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