Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease
Само за регистроване кориснике
2014
Аутори
Stanković, Milena N.Mladenović, Dušan
Đuričić, Ivana
Šobajić, Slađana
Timić, Jasmina
Jorgačević, Bojan
Aleksić, Vuk
Vucević, Danijela B.
Jesić-Vukicević, Rada
Radosavljević, Tatjana S.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background and Aims. Methionine-choline deficient (MCD) diet duration necessary for development of non-alcoholic fatty liver disease (NAFLD) and the dynamic of lipid profile and fatty acids are not completely established. The study examined dynamics and association between liver free fatty acids (FFA), serum lipid profile and liver morphological changes on MCD diet-induced NAFLD in mice. Methods. Male C57BL/6 mice (n = 28) were divided into four groups (n = 7 per group): control: fed with standard chow, MCD diet-fed groups: 2, 4 or 6 weeks. After treatment, liver and blood samples were taken for histopathology, serum lipid profile, and liver FFA composition. Results. Hepatic FFA profile showed a decrease in saturated acids, arachidonic and docosahexaenoic acid, whereas proportions of docosapentaenoic, oleic and linoleic acid were increased. Total cholesterol, HDL and triglycerides progressively decreased, whereas LDL level progressively increased. Focal fatty change in the liver appear...ed after 2 weeks, whereas diffuse fatty change with severe inflammation and ballooned hepatocytes were evident after 6 weeks. Conclusions. Six-week diet model may be appropriate for investigation of the role of lipotoxicity in the progression of NAFLD. Therefore, supplementation with n-3 polyunsaturated acid like DHA, rather than DPA, especially in the initial stage of fatty liver disease, may potentially have preventive effects and alleviate development of NAFLD/NASH and may also potentially reduce cardiovascular risk by moderating dyslipidemia.
Кључне речи:
Free fatty acids / Lipid status / Methionine-choline deficient diet / NAFLD / MiceИзвор:
Archives of Medical Research, 2014, 45, 2, 116-124Издавач:
- Elsevier Science Inc, New York
Финансирање / пројекти:
- Улога неуроендокрино-инфламаторне осовине у патогенези неалкохолне масне болести јетре (RS-MESTD-Basic Research (BR or ON)-175015)
DOI: 10.1016/j.arcmed.2013.12.010
ISSN: 0188-4409
PubMed: 24480733
WoS: 000333139100002
Scopus: 2-s2.0-84896879418
Институција/група
PharmacyTY - JOUR AU - Stanković, Milena N. AU - Mladenović, Dušan AU - Đuričić, Ivana AU - Šobajić, Slađana AU - Timić, Jasmina AU - Jorgačević, Bojan AU - Aleksić, Vuk AU - Vucević, Danijela B. AU - Jesić-Vukicević, Rada AU - Radosavljević, Tatjana S. PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2153 AB - Background and Aims. Methionine-choline deficient (MCD) diet duration necessary for development of non-alcoholic fatty liver disease (NAFLD) and the dynamic of lipid profile and fatty acids are not completely established. The study examined dynamics and association between liver free fatty acids (FFA), serum lipid profile and liver morphological changes on MCD diet-induced NAFLD in mice. Methods. Male C57BL/6 mice (n = 28) were divided into four groups (n = 7 per group): control: fed with standard chow, MCD diet-fed groups: 2, 4 or 6 weeks. After treatment, liver and blood samples were taken for histopathology, serum lipid profile, and liver FFA composition. Results. Hepatic FFA profile showed a decrease in saturated acids, arachidonic and docosahexaenoic acid, whereas proportions of docosapentaenoic, oleic and linoleic acid were increased. Total cholesterol, HDL and triglycerides progressively decreased, whereas LDL level progressively increased. Focal fatty change in the liver appeared after 2 weeks, whereas diffuse fatty change with severe inflammation and ballooned hepatocytes were evident after 6 weeks. Conclusions. Six-week diet model may be appropriate for investigation of the role of lipotoxicity in the progression of NAFLD. Therefore, supplementation with n-3 polyunsaturated acid like DHA, rather than DPA, especially in the initial stage of fatty liver disease, may potentially have preventive effects and alleviate development of NAFLD/NASH and may also potentially reduce cardiovascular risk by moderating dyslipidemia. PB - Elsevier Science Inc, New York T2 - Archives of Medical Research T1 - Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease VL - 45 IS - 2 SP - 116 EP - 124 DO - 10.1016/j.arcmed.2013.12.010 ER -
@article{ author = "Stanković, Milena N. and Mladenović, Dušan and Đuričić, Ivana and Šobajić, Slađana and Timić, Jasmina and Jorgačević, Bojan and Aleksić, Vuk and Vucević, Danijela B. and Jesić-Vukicević, Rada and Radosavljević, Tatjana S.", year = "2014", abstract = "Background and Aims. Methionine-choline deficient (MCD) diet duration necessary for development of non-alcoholic fatty liver disease (NAFLD) and the dynamic of lipid profile and fatty acids are not completely established. The study examined dynamics and association between liver free fatty acids (FFA), serum lipid profile and liver morphological changes on MCD diet-induced NAFLD in mice. Methods. Male C57BL/6 mice (n = 28) were divided into four groups (n = 7 per group): control: fed with standard chow, MCD diet-fed groups: 2, 4 or 6 weeks. After treatment, liver and blood samples were taken for histopathology, serum lipid profile, and liver FFA composition. Results. Hepatic FFA profile showed a decrease in saturated acids, arachidonic and docosahexaenoic acid, whereas proportions of docosapentaenoic, oleic and linoleic acid were increased. Total cholesterol, HDL and triglycerides progressively decreased, whereas LDL level progressively increased. Focal fatty change in the liver appeared after 2 weeks, whereas diffuse fatty change with severe inflammation and ballooned hepatocytes were evident after 6 weeks. Conclusions. Six-week diet model may be appropriate for investigation of the role of lipotoxicity in the progression of NAFLD. Therefore, supplementation with n-3 polyunsaturated acid like DHA, rather than DPA, especially in the initial stage of fatty liver disease, may potentially have preventive effects and alleviate development of NAFLD/NASH and may also potentially reduce cardiovascular risk by moderating dyslipidemia.", publisher = "Elsevier Science Inc, New York", journal = "Archives of Medical Research", title = "Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease", volume = "45", number = "2", pages = "116-124", doi = "10.1016/j.arcmed.2013.12.010" }
Stanković, M. N., Mladenović, D., Đuričić, I., Šobajić, S., Timić, J., Jorgačević, B., Aleksić, V., Vucević, D. B., Jesić-Vukicević, R.,& Radosavljević, T. S.. (2014). Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease. in Archives of Medical Research Elsevier Science Inc, New York., 45(2), 116-124. https://doi.org/10.1016/j.arcmed.2013.12.010
Stanković MN, Mladenović D, Đuričić I, Šobajić S, Timić J, Jorgačević B, Aleksić V, Vucević DB, Jesić-Vukicević R, Radosavljević TS. Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease. in Archives of Medical Research. 2014;45(2):116-124. doi:10.1016/j.arcmed.2013.12.010 .
Stanković, Milena N., Mladenović, Dušan, Đuričić, Ivana, Šobajić, Slađana, Timić, Jasmina, Jorgačević, Bojan, Aleksić, Vuk, Vucević, Danijela B., Jesić-Vukicević, Rada, Radosavljević, Tatjana S., "Time-dependent Changes and Association Between Liver Free Fatty Acids, Serum Lipid Profile and Histological Features in Mice Model of Nonalcoholic Fatty Liver Disease" in Archives of Medical Research, 45, no. 2 (2014):116-124, https://doi.org/10.1016/j.arcmed.2013.12.010 . .