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Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes

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2014
Authors
Pirković, Andrea
Živković, Lada
Topalović, Dijana
Đelić, Ninoslav
Bajić, Vladan
Dekanski, Dragana
Potparević, Biljana
Article (Published version)
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Abstract
Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P lt 0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation anal...ysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P lt 0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.

Keywords:
Dry olive leaf extract (DOLE), Adrenaline / (CAS 329-63-5) / Oxidative stress / Comet assay
Source:
Toxicology Letters, 2014, 28, 3, 451-456
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Projects:
  • Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (RS-173034)

DOI: 10.1016/j.tiv.2013.12.014

ISSN: 0887-2333

PubMed: 24389114

WoS: 000332053800015

Scopus: 2-s2.0-84892897826
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2156
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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