Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P lt 0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation anal...ysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P lt 0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.
TY - JOUR
AU - Pirković, Andrea
AU - Živković, Lada
AU - Topalović, Dijana
AU - Đelić, Ninoslav
AU - Bajić, Vladan
AU - Dekanski, Dragana
AU - Potparević, Biljana
PY - 2014
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2156
AB - Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P lt 0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P lt 0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Toxicology Letters
T1 - Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes
VL - 28
IS - 3
SP - 451
EP - 456
DO - 10.1016/j.tiv.2013.12.014
ER -
@article{
author = "Pirković, Andrea and Živković, Lada and Topalović, Dijana and Đelić, Ninoslav and Bajić, Vladan and Dekanski, Dragana and Potparević, Biljana",
year = "2014",
abstract = "Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P lt 0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P lt 0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicology Letters",
title = "Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes",
volume = "28",
number = "3",
pages = "451-456",
doi = "10.1016/j.tiv.2013.12.014"
}
Pirković, A., Živković, L., Topalović, D., Đelić, N., Bajić, V., Dekanski, D.,& Potparević, B.. (2014). Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. in Toxicology Letters
Pergamon-Elsevier Science Ltd, Oxford., 28(3), 451-456.
https://doi.org/10.1016/j.tiv.2013.12.014
Pirković A, Živković L, Topalović D, Đelić N, Bajić V, Dekanski D, Potparević B. Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. in Toxicology Letters. 2014;28(3):451-456.
doi:10.1016/j.tiv.2013.12.014 .
Pirković, Andrea, Živković, Lada, Topalović, Dijana, Đelić, Ninoslav, Bajić, Vladan, Dekanski, Dragana, Potparević, Biljana, "Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes" in Toxicology Letters, 28, no. 3 (2014):451-456,
https://doi.org/10.1016/j.tiv.2013.12.014 . .
