Show simple item record

dc.creatorPirković, Andrea
dc.creatorŽivković, Lada
dc.creatorTopalović, Dijana
dc.creatorĐelić, Ninoslav
dc.creatorBajić, Vladan
dc.creatorDekanski, Dragana
dc.creatorPotparević, Biljana
dc.date.accessioned2019-09-02T11:40:27Z
dc.date.available2019-09-02T11:40:27Z
dc.date.issued2014
dc.identifier.issn0887-2333
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2156
dc.description.abstractExcessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P lt 0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P lt 0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173034/RS//
dc.rightsrestrictedAccess
dc.sourceToxicology Letters
dc.subjectDry olive leaf extract (DOLE), Adrenalineen
dc.subject(CAS 329-63-5)en
dc.subjectOxidative stressen
dc.subjectComet assayen
dc.titleProtective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЂелић, Нинослав; Пирковић, Aндреа; Бајић, Владан; Живковић, Лада; Декански, Драгана; Топаловић, Дијана; Потпаревић, Биљана;
dc.citation.volume28
dc.citation.issue3
dc.citation.spage451
dc.citation.epage456
dc.citation.other28(3): 451-456
dc.citation.rankM22
dc.identifier.wos000332053800015
dc.identifier.doi10.1016/j.tiv.2013.12.014
dc.identifier.pmid24389114
dc.identifier.scopus2-s2.0-84892897826
dc.identifier.rcubconv_3028
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record