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Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients

Authorized Users Only
2014
Authors
Vekić, Jelena
Zeljković, Aleksandra
Jelić-Ivanović, Zorana
Damjanović, Tatjana
Šuvakov, Sonja
Matić, Marija
Savić-Radojević, Ana
Simić, Tatjana
Spasojević-Kalimanovska, Vesna
Gojković, Tamara
Spasić, Slavica
Dimković, Nada
Article (Published version)
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Abstract
Objectives: End-stage renal disease (ESRD) is characterized by profound dyslipidemia and enhanced oxidative stress. The patients also show evidence of exhausted and/or deficient anti-oxidative defense enzymes, one of them being glutathione-S-transferase (GST). This study investigates relationship between GST gene polymorphism and low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses in ESRD. Design and methods: GSTM1, T1, and P1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 160 patients undergoing hemodialysis. LDL and HDL subclasses were separated by gradient gel electrophoresis and biochemical parameters were measured by routine laboratory methods. Results: GSTM1-positive patients had higher proportion of small, dense LDL III particles than those with GSTM1-null genotype (P lt 0.05). Similarly, GSTP1-Ile/Ile patients had higher proportion of LDL III (P lt 0.05), but more HDL 2b and less HDL 3a particles th...an GSTP1-Ile/Val and Val/Val carriers (P lt 0.05). LDL subclass distribution in smokers with GSTM1-null genotype was shifted towards smaller particles, as compared to GSTM1-positive and GSTM1-null non-smokers. Smokers with GSTP1-Ile/Val and Val/Val genotypes had smaller LDL size than their non-smoking counterparts (P lt 0.05). Both smokers and non-smokers with GSTP1 Ile/Ile genotype had more LDL III particles than non-smokers carrying Val allele. Non-smokers with GSTP1 Ile/Ile genotype had more HDL 2b subclasses than non-smokers with GSTP1-Ile/Val and Val/Val (P lt 0.05), but less HDL 3a particles than smokers with GSTP1-Ile/Val and Val/Val genotypes (P lt 0.05). GSTT1 gene polymorphism had no effect on lipoprotein subclass distributions. Conclusions: Our results demonstrate significant associations between low activity GST genotypes and proatherogenic lipoprotein particles in hemodialysis patients which might further increase their cardiovascular disease risk.

Keywords:
Glutathione-S-transferase polymorphism / Small / Dense LDL / HDL subclasses / End-stage renal disease / Cardiovascular disease risk
Source:
Clinical Biochemistry, 2014, 47, 6, 398-403
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
  • Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers (RS-175035)

DOI: 10.1016/j.clinbiochem.2013.11.011

ISSN: 0009-9120

PubMed: 24291050

WoS: 000333763300014

Scopus: 2-s2.0-84897112913
[ Google Scholar ]
4
3
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/2157
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Jelić-Ivanović, Zorana
AU  - Damjanović, Tatjana
AU  - Šuvakov, Sonja
AU  - Matić, Marija
AU  - Savić-Radojević, Ana
AU  - Simić, Tatjana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Gojković, Tamara
AU  - Spasić, Slavica
AU  - Dimković, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2157
AB  - Objectives: End-stage renal disease (ESRD) is characterized by profound dyslipidemia and enhanced oxidative stress. The patients also show evidence of exhausted and/or deficient anti-oxidative defense enzymes, one of them being glutathione-S-transferase (GST). This study investigates relationship between GST gene polymorphism and low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses in ESRD. Design and methods: GSTM1, T1, and P1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 160 patients undergoing hemodialysis. LDL and HDL subclasses were separated by gradient gel electrophoresis and biochemical parameters were measured by routine laboratory methods. Results: GSTM1-positive patients had higher proportion of small, dense LDL III particles than those with GSTM1-null genotype (P  lt  0.05). Similarly, GSTP1-Ile/Ile patients had higher proportion of LDL III (P  lt  0.05), but more HDL 2b and less HDL 3a particles than GSTP1-Ile/Val and Val/Val carriers (P  lt  0.05). LDL subclass distribution in smokers with GSTM1-null genotype was shifted towards smaller particles, as compared to GSTM1-positive and GSTM1-null non-smokers. Smokers with GSTP1-Ile/Val and Val/Val genotypes had smaller LDL size than their non-smoking counterparts (P  lt  0.05). Both smokers and non-smokers with GSTP1 Ile/Ile genotype had more LDL III particles than non-smokers carrying Val allele. Non-smokers with GSTP1 Ile/Ile genotype had more HDL 2b subclasses than non-smokers with GSTP1-Ile/Val and Val/Val (P  lt  0.05), but less HDL 3a particles than smokers with GSTP1-Ile/Val and Val/Val genotypes (P  lt  0.05). GSTT1 gene polymorphism had no effect on lipoprotein subclass distributions. Conclusions: Our results demonstrate significant associations between low activity GST genotypes and proatherogenic lipoprotein particles in hemodialysis patients which might further increase their cardiovascular disease risk.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients
VL  - 47
IS  - 6
SP  - 398
EP  - 403
DO  - 10.1016/j.clinbiochem.2013.11.011
ER  - 
@article{
author = "Vekić, Jelena and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Damjanović, Tatjana and Šuvakov, Sonja and Matić, Marija and Savić-Radojević, Ana and Simić, Tatjana and Spasojević-Kalimanovska, Vesna and Gojković, Tamara and Spasić, Slavica and Dimković, Nada",
year = "2014",
abstract = "Objectives: End-stage renal disease (ESRD) is characterized by profound dyslipidemia and enhanced oxidative stress. The patients also show evidence of exhausted and/or deficient anti-oxidative defense enzymes, one of them being glutathione-S-transferase (GST). This study investigates relationship between GST gene polymorphism and low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses in ESRD. Design and methods: GSTM1, T1, and P1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 160 patients undergoing hemodialysis. LDL and HDL subclasses were separated by gradient gel electrophoresis and biochemical parameters were measured by routine laboratory methods. Results: GSTM1-positive patients had higher proportion of small, dense LDL III particles than those with GSTM1-null genotype (P  lt  0.05). Similarly, GSTP1-Ile/Ile patients had higher proportion of LDL III (P  lt  0.05), but more HDL 2b and less HDL 3a particles than GSTP1-Ile/Val and Val/Val carriers (P  lt  0.05). LDL subclass distribution in smokers with GSTM1-null genotype was shifted towards smaller particles, as compared to GSTM1-positive and GSTM1-null non-smokers. Smokers with GSTP1-Ile/Val and Val/Val genotypes had smaller LDL size than their non-smoking counterparts (P  lt  0.05). Both smokers and non-smokers with GSTP1 Ile/Ile genotype had more LDL III particles than non-smokers carrying Val allele. Non-smokers with GSTP1 Ile/Ile genotype had more HDL 2b subclasses than non-smokers with GSTP1-Ile/Val and Val/Val (P  lt  0.05), but less HDL 3a particles than smokers with GSTP1-Ile/Val and Val/Val genotypes (P  lt  0.05). GSTT1 gene polymorphism had no effect on lipoprotein subclass distributions. Conclusions: Our results demonstrate significant associations between low activity GST genotypes and proatherogenic lipoprotein particles in hemodialysis patients which might further increase their cardiovascular disease risk.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients",
volume = "47",
number = "6",
pages = "398-403",
doi = "10.1016/j.clinbiochem.2013.11.011"
}
Vekić, J., Zeljković, A., Jelić-Ivanović, Z., Damjanović, T., Šuvakov, S., Matić, M., Savić-Radojević, A., Simić, T., Spasojević-Kalimanovska, V., Gojković, T., Spasić, S.,& Dimković, N.. (2014). Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 47(6), 398-403.
https://doi.org/10.1016/j.clinbiochem.2013.11.011
Vekić J, Zeljković A, Jelić-Ivanović Z, Damjanović T, Šuvakov S, Matić M, Savić-Radojević A, Simić T, Spasojević-Kalimanovska V, Gojković T, Spasić S, Dimković N. Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients. in Clinical Biochemistry. 2014;47(6):398-403.
doi:10.1016/j.clinbiochem.2013.11.011 .
Vekić, Jelena, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Damjanović, Tatjana, Šuvakov, Sonja, Matić, Marija, Savić-Radojević, Ana, Simić, Tatjana, Spasojević-Kalimanovska, Vesna, Gojković, Tamara, Spasić, Slavica, Dimković, Nada, "Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients" in Clinical Biochemistry, 47, no. 6 (2014):398-403,
https://doi.org/10.1016/j.clinbiochem.2013.11.011 . .

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