Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients
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2014
Authors
Vekić, Jelena
Zeljković, Aleksandra

Jelić-Ivanović, Zorana

Damjanović, Tatjana
Šuvakov, Sonja
Matić, Marija
Savić-Radojević, Ana
Simić, Tatjana

Spasojević-Kalimanovska, Vesna

Gojković, Tamara

Spasić, Slavica
Dimković, Nada
Article (Published version)

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Objectives: End-stage renal disease (ESRD) is characterized by profound dyslipidemia and enhanced oxidative stress. The patients also show evidence of exhausted and/or deficient anti-oxidative defense enzymes, one of them being glutathione-S-transferase (GST). This study investigates relationship between GST gene polymorphism and low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses in ESRD. Design and methods: GSTM1, T1, and P1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 160 patients undergoing hemodialysis. LDL and HDL subclasses were separated by gradient gel electrophoresis and biochemical parameters were measured by routine laboratory methods. Results: GSTM1-positive patients had higher proportion of small, dense LDL III particles than those with GSTM1-null genotype (P lt 0.05). Similarly, GSTP1-Ile/Ile patients had higher proportion of LDL III (P lt 0.05), but more HDL 2b and less HDL 3a particles th...an GSTP1-Ile/Val and Val/Val carriers (P lt 0.05). LDL subclass distribution in smokers with GSTM1-null genotype was shifted towards smaller particles, as compared to GSTM1-positive and GSTM1-null non-smokers. Smokers with GSTP1-Ile/Val and Val/Val genotypes had smaller LDL size than their non-smoking counterparts (P lt 0.05). Both smokers and non-smokers with GSTP1 Ile/Ile genotype had more LDL III particles than non-smokers carrying Val allele. Non-smokers with GSTP1 Ile/Ile genotype had more HDL 2b subclasses than non-smokers with GSTP1-Ile/Val and Val/Val (P lt 0.05), but less HDL 3a particles than smokers with GSTP1-Ile/Val and Val/Val genotypes (P lt 0.05). GSTT1 gene polymorphism had no effect on lipoprotein subclass distributions. Conclusions: Our results demonstrate significant associations between low activity GST genotypes and proatherogenic lipoprotein particles in hemodialysis patients which might further increase their cardiovascular disease risk.
Keywords:
Glutathione-S-transferase polymorphism / Small / Dense LDL / HDL subclasses / End-stage renal disease / Cardiovascular disease riskSource:
Clinical Biochemistry, 2014, 47, 6, 398-403Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
DOI: 10.1016/j.clinbiochem.2013.11.011
ISSN: 0009-9120
PubMed: 24291050
WoS: 000333763300014
Scopus: 2-s2.0-84897112913
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PharmacyTY - JOUR AU - Vekić, Jelena AU - Zeljković, Aleksandra AU - Jelić-Ivanović, Zorana AU - Damjanović, Tatjana AU - Šuvakov, Sonja AU - Matić, Marija AU - Savić-Radojević, Ana AU - Simić, Tatjana AU - Spasojević-Kalimanovska, Vesna AU - Gojković, Tamara AU - Spasić, Slavica AU - Dimković, Nada PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2157 AB - Objectives: End-stage renal disease (ESRD) is characterized by profound dyslipidemia and enhanced oxidative stress. The patients also show evidence of exhausted and/or deficient anti-oxidative defense enzymes, one of them being glutathione-S-transferase (GST). This study investigates relationship between GST gene polymorphism and low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses in ESRD. Design and methods: GSTM1, T1, and P1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 160 patients undergoing hemodialysis. LDL and HDL subclasses were separated by gradient gel electrophoresis and biochemical parameters were measured by routine laboratory methods. Results: GSTM1-positive patients had higher proportion of small, dense LDL III particles than those with GSTM1-null genotype (P lt 0.05). Similarly, GSTP1-Ile/Ile patients had higher proportion of LDL III (P lt 0.05), but more HDL 2b and less HDL 3a particles than GSTP1-Ile/Val and Val/Val carriers (P lt 0.05). LDL subclass distribution in smokers with GSTM1-null genotype was shifted towards smaller particles, as compared to GSTM1-positive and GSTM1-null non-smokers. Smokers with GSTP1-Ile/Val and Val/Val genotypes had smaller LDL size than their non-smoking counterparts (P lt 0.05). Both smokers and non-smokers with GSTP1 Ile/Ile genotype had more LDL III particles than non-smokers carrying Val allele. Non-smokers with GSTP1 Ile/Ile genotype had more HDL 2b subclasses than non-smokers with GSTP1-Ile/Val and Val/Val (P lt 0.05), but less HDL 3a particles than smokers with GSTP1-Ile/Val and Val/Val genotypes (P lt 0.05). GSTT1 gene polymorphism had no effect on lipoprotein subclass distributions. Conclusions: Our results demonstrate significant associations between low activity GST genotypes and proatherogenic lipoprotein particles in hemodialysis patients which might further increase their cardiovascular disease risk. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Clinical Biochemistry T1 - Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients VL - 47 IS - 6 SP - 398 EP - 403 DO - 10.1016/j.clinbiochem.2013.11.011 ER -
@article{ author = "Vekić, Jelena and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Damjanović, Tatjana and Šuvakov, Sonja and Matić, Marija and Savić-Radojević, Ana and Simić, Tatjana and Spasojević-Kalimanovska, Vesna and Gojković, Tamara and Spasić, Slavica and Dimković, Nada", year = "2014", abstract = "Objectives: End-stage renal disease (ESRD) is characterized by profound dyslipidemia and enhanced oxidative stress. The patients also show evidence of exhausted and/or deficient anti-oxidative defense enzymes, one of them being glutathione-S-transferase (GST). This study investigates relationship between GST gene polymorphism and low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses in ESRD. Design and methods: GSTM1, T1, and P1 genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 160 patients undergoing hemodialysis. LDL and HDL subclasses were separated by gradient gel electrophoresis and biochemical parameters were measured by routine laboratory methods. Results: GSTM1-positive patients had higher proportion of small, dense LDL III particles than those with GSTM1-null genotype (P lt 0.05). Similarly, GSTP1-Ile/Ile patients had higher proportion of LDL III (P lt 0.05), but more HDL 2b and less HDL 3a particles than GSTP1-Ile/Val and Val/Val carriers (P lt 0.05). LDL subclass distribution in smokers with GSTM1-null genotype was shifted towards smaller particles, as compared to GSTM1-positive and GSTM1-null non-smokers. Smokers with GSTP1-Ile/Val and Val/Val genotypes had smaller LDL size than their non-smoking counterparts (P lt 0.05). Both smokers and non-smokers with GSTP1 Ile/Ile genotype had more LDL III particles than non-smokers carrying Val allele. Non-smokers with GSTP1 Ile/Ile genotype had more HDL 2b subclasses than non-smokers with GSTP1-Ile/Val and Val/Val (P lt 0.05), but less HDL 3a particles than smokers with GSTP1-Ile/Val and Val/Val genotypes (P lt 0.05). GSTT1 gene polymorphism had no effect on lipoprotein subclass distributions. Conclusions: Our results demonstrate significant associations between low activity GST genotypes and proatherogenic lipoprotein particles in hemodialysis patients which might further increase their cardiovascular disease risk.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Clinical Biochemistry", title = "Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients", volume = "47", number = "6", pages = "398-403", doi = "10.1016/j.clinbiochem.2013.11.011" }
Vekić, J., Zeljković, A., Jelić-Ivanović, Z., Damjanović, T., Šuvakov, S., Matić, M., Savić-Radojević, A., Simić, T., Spasojević-Kalimanovska, V., Gojković, T., Spasić, S.,& Dimković, N.. (2014). Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients. in Clinical Biochemistry Pergamon-Elsevier Science Ltd, Oxford., 47(6), 398-403. https://doi.org/10.1016/j.clinbiochem.2013.11.011
Vekić J, Zeljković A, Jelić-Ivanović Z, Damjanović T, Šuvakov S, Matić M, Savić-Radojević A, Simić T, Spasojević-Kalimanovska V, Gojković T, Spasić S, Dimković N. Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients. in Clinical Biochemistry. 2014;47(6):398-403. doi:10.1016/j.clinbiochem.2013.11.011 .
Vekić, Jelena, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Damjanović, Tatjana, Šuvakov, Sonja, Matić, Marija, Savić-Radojević, Ana, Simić, Tatjana, Spasojević-Kalimanovska, Vesna, Gojković, Tamara, Spasić, Slavica, Dimković, Nada, "Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients" in Clinical Biochemistry, 47, no. 6 (2014):398-403, https://doi.org/10.1016/j.clinbiochem.2013.11.011 . .