FarFaR - Farmaceutski fakultet, repozitorijum
Univerzitet u Beogradu, Farmaceutski fakultet
    • English
    • Српски
    • Српски (Serbia)
  • Srpski (latinica) 
    • Engleski
    • Srpski (ćirilica)
    • Srpski (latinica)
  • Prijava
Pregled rada 
  •   FarFaR - Farmaceutski fakultet, repozitorijum
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • Pregled rada
  •   FarFaR - Farmaceutski fakultet, repozitorijum
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • Pregled rada
JavaScript is disabled for your browser. Some features of this site may not work without it.

Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells

Thumbnail
2014
2167.pdf (2.622Mb)
Autori
Milićević, Zorka
Bajić, Vladan
Živković, Lada
Kasapović, Jelena
Anđelković, Uroš
Potparević, Biljana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) ...and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.

Izvor:
Sensors, 2014
Izdavač:
  • Hindawi Publishing Corp, New York
Projekti:
  • Aberacije ćelijskog ciklusa i uticaj oksidativnog stresa na neurodegenerativne procese i malignu transformaciju ćelije (RS-173034)

DOI: 10.1155/2014/618698

ISSN: 1537-744X

PubMed: 24511294

WoS: 000330388500001

Scopus: 2-s2.0-84893205816
[ Google Scholar ]
13
11
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2169
Kolekcije
  • Radovi istraživača / Researchers’ publications
Institucija
Pharmacy
TY  - JOUR
AU  - Milićević, Zorka
AU  - Bajić, Vladan
AU  - Živković, Lada
AU  - Kasapović, Jelena
AU  - Anđelković, Uroš
AU  - Potparević, Biljana
PY  - 2014
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2169
AB  - In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.
PB  - Hindawi Publishing Corp, New York
T2  - Sensors
T1  - Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells
DO  - 10.1155/2014/618698
ER  - 
@article{
author = "Milićević, Zorka and Bajić, Vladan and Živković, Lada and Kasapović, Jelena and Anđelković, Uroš and Potparević, Biljana",
year = "2014",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/2169",
abstract = "In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.",
publisher = "Hindawi Publishing Corp, New York",
journal = "Sensors",
title = "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells",
doi = "10.1155/2014/618698"
}
Milićević Z, Bajić V, Živković L, Kasapović J, Anđelković U, Potparević B. Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. Sensors. 2014;
Milićević, Z., Bajić, V., Živković, L., Kasapović, J., Anđelković, U.,& Potparević, B. (2014). Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells.
SensorsHindawi Publishing Corp, New York..
https://doi.org/10.1155/2014/618698
Milićević Zorka, Bajić Vladan, Živković Lada, Kasapović Jelena, Anđelković Uroš, Potparević Biljana, "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells" (2014),
https://doi.org/10.1155/2014/618698 .

DSpace software copyright © 2002-2015  DuraSpace
O FarFaR-u | Pošaljite zapažanja

OpenAIRERCUB
 

 

Kompletan repozitorijumInstitucijeAutoriNasloviTemeOva institucijaAutoriNasloviTeme

Statistika

Pregled statistika

DSpace software copyright © 2002-2015  DuraSpace
O FarFaR-u | Pošaljite zapažanja

OpenAIRERCUB