New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83.
TY - JOUR
AU - Jovčić, Branko
AU - Lepsanović, Zorica
AU - Begović, Jelena
AU - Filipić, Brankica
AU - Kojić, Milan
PY - 2014
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2173
AB - New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83.
PB - Springer, Dordrecht
T2 - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1 - Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia
VL - 105
IS - 3
SP - 613
EP - 618
DO - 10.1007/s10482-013-0094-z
ER -
@article{
author = "Jovčić, Branko and Lepsanović, Zorica and Begović, Jelena and Filipić, Brankica and Kojić, Milan",
year = "2014",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/2173",
abstract = "New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia",
volume = "105",
number = "3",
pages = "613-618",
doi = "10.1007/s10482-013-0094-z"
}
Jovčić, B., Lepsanović, Z., Begović, J., Filipić, B.,& Kojić, M. (2014). Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia.
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 105(3), 613-618.
https://doi.org/10.1007/s10482-013-0094-z
Jovčić B, Lepsanović Z, Begović J, Filipić B, Kojić M. Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2014;105(3):613-618
Jovčić Branko, Lepsanović Zorica, Begović Jelena, Filipić Brankica, Kojić Milan, "Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia" Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 105, no. 3 (2014):613-618,
https://doi.org/10.1007/s10482-013-0094-z .
