The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD
2014
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Authors
Stanković, Milena N.Mladenović, Dušan
Ninković, Milica
Đuričić, Ivana
Šobajić, Slađana
Jorgacević, Bojan
de Luka, Silvio
Jesić-Vukicević, Rada
Radosavljević, Tatjana S.
Article (Published version)
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Development of nonalcoholic fatty liver disease (NAFLD) occurs through initial steatosis and subsequent oxidative stress. The aim of this study was to examine the effects of -lipoic acid (LA) on methionine-choline deficient (MCD) diet-induced NAFLD in mice. Male C57BL/6 mice (n=21) were divided into three groups (n=7 per group): (1) control fed with standard chow, (2) MCD2 groupfed with MCD diet for 2 weeks, and (3) MCD2+LA group2 weeks on MCD receiving LA i.p. 100mg/kg/day. After the treatment, liver samples were taken for pathohistology, oxidative stress parameters, antioxidative enzymes, and liver free fatty acid (FFA) composition. Mild microvesicular hepatic steatosis was found in MCD2 group, while it was reduced to single fat droplets evident in MCD2+LA group. Lipid peroxidation and nitrosative stress were increased by MCD diet, while LA administration induced a decrease in liver malondialdehyde and nitrates+nitrites level. Similary, LA improved liver antioxidative capacity by inc...reasing total superoxide dismutase (tSOD), manganese SOD (MnSOD), and copper/zinc-SOD (Cu/ZnSOD) activity as well as glutathione (GSH) content. Liver FFA profile has shown a significant decrease in saturated acids, arachidonic, and docosahexaenoic acid (DHA), while LA treatment increased their proportions. It can be concluded that LA ameliorates lipid peroxidation and nitrosative stress in MCD diet-induced hepatic steatosis through an increase in SOD activity and GSH level. In addition, LA increases the proportion of palmitic, stearic, arachidonic, and DHA in the fatty liver. An increase in DHA may be a potential mechanism of anti-inflammatory and antioxidant effects of LA in MCD diet-induced NAFLD.
Keywords:
antioxidant / DHA / FFA / lipid peroxidation / lipotoxicity / liver / mice / steatosisSource:
Journal of Medicinal Food, 2014, 17, 2, 254-261Publisher:
- Mary Ann Liebert, Inc, New Rochelle
Funding / projects:
- The role of neuroendocrine-inflammatory axis in the pathogenesis of non-alcoholic fatty liver disease (RS-MESTD-Basic Research (BR or ON)-175015)
DOI: 10.1089/jmf.2013.0111
ISSN: 1096-620X
PubMed: 24325457
WoS: 000331394000009
Scopus: 2-s2.0-84894490378
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PharmacyTY - JOUR AU - Stanković, Milena N. AU - Mladenović, Dušan AU - Ninković, Milica AU - Đuričić, Ivana AU - Šobajić, Slađana AU - Jorgacević, Bojan AU - de Luka, Silvio AU - Jesić-Vukicević, Rada AU - Radosavljević, Tatjana S. PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2176 AB - Development of nonalcoholic fatty liver disease (NAFLD) occurs through initial steatosis and subsequent oxidative stress. The aim of this study was to examine the effects of -lipoic acid (LA) on methionine-choline deficient (MCD) diet-induced NAFLD in mice. Male C57BL/6 mice (n=21) were divided into three groups (n=7 per group): (1) control fed with standard chow, (2) MCD2 groupfed with MCD diet for 2 weeks, and (3) MCD2+LA group2 weeks on MCD receiving LA i.p. 100mg/kg/day. After the treatment, liver samples were taken for pathohistology, oxidative stress parameters, antioxidative enzymes, and liver free fatty acid (FFA) composition. Mild microvesicular hepatic steatosis was found in MCD2 group, while it was reduced to single fat droplets evident in MCD2+LA group. Lipid peroxidation and nitrosative stress were increased by MCD diet, while LA administration induced a decrease in liver malondialdehyde and nitrates+nitrites level. Similary, LA improved liver antioxidative capacity by increasing total superoxide dismutase (tSOD), manganese SOD (MnSOD), and copper/zinc-SOD (Cu/ZnSOD) activity as well as glutathione (GSH) content. Liver FFA profile has shown a significant decrease in saturated acids, arachidonic, and docosahexaenoic acid (DHA), while LA treatment increased their proportions. It can be concluded that LA ameliorates lipid peroxidation and nitrosative stress in MCD diet-induced hepatic steatosis through an increase in SOD activity and GSH level. In addition, LA increases the proportion of palmitic, stearic, arachidonic, and DHA in the fatty liver. An increase in DHA may be a potential mechanism of anti-inflammatory and antioxidant effects of LA in MCD diet-induced NAFLD. PB - Mary Ann Liebert, Inc, New Rochelle T2 - Journal of Medicinal Food T1 - The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD VL - 17 IS - 2 SP - 254 EP - 261 DO - 10.1089/jmf.2013.0111 ER -
@article{ author = "Stanković, Milena N. and Mladenović, Dušan and Ninković, Milica and Đuričić, Ivana and Šobajić, Slađana and Jorgacević, Bojan and de Luka, Silvio and Jesić-Vukicević, Rada and Radosavljević, Tatjana S.", year = "2014", abstract = "Development of nonalcoholic fatty liver disease (NAFLD) occurs through initial steatosis and subsequent oxidative stress. The aim of this study was to examine the effects of -lipoic acid (LA) on methionine-choline deficient (MCD) diet-induced NAFLD in mice. Male C57BL/6 mice (n=21) were divided into three groups (n=7 per group): (1) control fed with standard chow, (2) MCD2 groupfed with MCD diet for 2 weeks, and (3) MCD2+LA group2 weeks on MCD receiving LA i.p. 100mg/kg/day. After the treatment, liver samples were taken for pathohistology, oxidative stress parameters, antioxidative enzymes, and liver free fatty acid (FFA) composition. Mild microvesicular hepatic steatosis was found in MCD2 group, while it was reduced to single fat droplets evident in MCD2+LA group. Lipid peroxidation and nitrosative stress were increased by MCD diet, while LA administration induced a decrease in liver malondialdehyde and nitrates+nitrites level. Similary, LA improved liver antioxidative capacity by increasing total superoxide dismutase (tSOD), manganese SOD (MnSOD), and copper/zinc-SOD (Cu/ZnSOD) activity as well as glutathione (GSH) content. Liver FFA profile has shown a significant decrease in saturated acids, arachidonic, and docosahexaenoic acid (DHA), while LA treatment increased their proportions. It can be concluded that LA ameliorates lipid peroxidation and nitrosative stress in MCD diet-induced hepatic steatosis through an increase in SOD activity and GSH level. In addition, LA increases the proportion of palmitic, stearic, arachidonic, and DHA in the fatty liver. An increase in DHA may be a potential mechanism of anti-inflammatory and antioxidant effects of LA in MCD diet-induced NAFLD.", publisher = "Mary Ann Liebert, Inc, New Rochelle", journal = "Journal of Medicinal Food", title = "The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD", volume = "17", number = "2", pages = "254-261", doi = "10.1089/jmf.2013.0111" }
Stanković, M. N., Mladenović, D., Ninković, M., Đuričić, I., Šobajić, S., Jorgacević, B., de Luka, S., Jesić-Vukicević, R.,& Radosavljević, T. S.. (2014). The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD. in Journal of Medicinal Food Mary Ann Liebert, Inc, New Rochelle., 17(2), 254-261. https://doi.org/10.1089/jmf.2013.0111
Stanković MN, Mladenović D, Ninković M, Đuričić I, Šobajić S, Jorgacević B, de Luka S, Jesić-Vukicević R, Radosavljević TS. The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD. in Journal of Medicinal Food. 2014;17(2):254-261. doi:10.1089/jmf.2013.0111 .
Stanković, Milena N., Mladenović, Dušan, Ninković, Milica, Đuričić, Ivana, Šobajić, Slađana, Jorgacević, Bojan, de Luka, Silvio, Jesić-Vukicević, Rada, Radosavljević, Tatjana S., "The Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLD" in Journal of Medicinal Food, 17, no. 2 (2014):254-261, https://doi.org/10.1089/jmf.2013.0111 . .