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dc.creatorStanković, Milena N.
dc.creatorMladenović, Dušan
dc.creatorNinković, Milica
dc.creatorĐuričić, Ivana
dc.creatorŠobajić, Slađana
dc.creatorJorgacević, Bojan
dc.creatorde Luka, Silvio
dc.creatorJesić-Vukicević, Rada
dc.creatorRadosavljević, Tatjana S.
dc.date.accessioned2019-09-02T11:40:56Z
dc.date.available2019-09-02T11:40:56Z
dc.date.issued2014
dc.identifier.issn1096-620X
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2176
dc.description.abstractDevelopment of nonalcoholic fatty liver disease (NAFLD) occurs through initial steatosis and subsequent oxidative stress. The aim of this study was to examine the effects of -lipoic acid (LA) on methionine-choline deficient (MCD) diet-induced NAFLD in mice. Male C57BL/6 mice (n=21) were divided into three groups (n=7 per group): (1) control fed with standard chow, (2) MCD2 groupfed with MCD diet for 2 weeks, and (3) MCD2+LA group2 weeks on MCD receiving LA i.p. 100mg/kg/day. After the treatment, liver samples were taken for pathohistology, oxidative stress parameters, antioxidative enzymes, and liver free fatty acid (FFA) composition. Mild microvesicular hepatic steatosis was found in MCD2 group, while it was reduced to single fat droplets evident in MCD2+LA group. Lipid peroxidation and nitrosative stress were increased by MCD diet, while LA administration induced a decrease in liver malondialdehyde and nitrates+nitrites level. Similary, LA improved liver antioxidative capacity by increasing total superoxide dismutase (tSOD), manganese SOD (MnSOD), and copper/zinc-SOD (Cu/ZnSOD) activity as well as glutathione (GSH) content. Liver FFA profile has shown a significant decrease in saturated acids, arachidonic, and docosahexaenoic acid (DHA), while LA treatment increased their proportions. It can be concluded that LA ameliorates lipid peroxidation and nitrosative stress in MCD diet-induced hepatic steatosis through an increase in SOD activity and GSH level. In addition, LA increases the proportion of palmitic, stearic, arachidonic, and DHA in the fatty liver. An increase in DHA may be a potential mechanism of anti-inflammatory and antioxidant effects of LA in MCD diet-induced NAFLD.en
dc.publisherMary Ann Liebert, Inc, New Rochelle
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175015/RS//
dc.rightsopenAccess
dc.sourceJournal of Medicinal Food
dc.subjectantioxidanten
dc.subjectDHAen
dc.subjectFFAen
dc.subjectlipid peroxidationen
dc.subjectlipotoxicityen
dc.subjectliveren
dc.subjectmiceen
dc.subjectsteatosisen
dc.titleThe Effects of alpha-Lipoic Acid on Liver Oxidative Stress and Free Fatty Acid Composition in Methionine-Choline Deficient Diet-Induced NAFLDen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractде Лука, Силвио; Шобајић, Слађана; Нинковић, Милица; Радосављевић, Татјана С.; Станковић, Милена Н.; Јоргацевић, Бојан; Јесић-Вукицевић, Рада; Младеновић, Душан; Ђуричић, Ивана;
dc.citation.volume17
dc.citation.issue2
dc.citation.spage254
dc.citation.epage261
dc.citation.other17(2): 254-261
dc.citation.rankM22
dc.identifier.wos000331394000009
dc.identifier.doi10.1089/jmf.2013.0111
dc.identifier.pmid24325457
dc.identifier.scopus2-s2.0-84894490378
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/902/2174.pdf
dc.identifier.rcubconv_3023
dc.type.versionpublishedVersion


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