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dc.creatorLeposavić, Gordana
dc.creatorPerišić-Nanut, Milica
dc.creatorPilipović, Ivan
dc.creatorKosec, Duško
dc.creatorArsenović-Ranin, Nevena
dc.creatorStojić-Vukanić, Zorica
dc.creatorĐikić, Jasrnina
dc.creatorNacka-Aleksić, Mirjana
dc.date.accessioned2019-09-02T11:41:02Z
dc.date.available2019-09-02T11:41:02Z
dc.date.issued2014
dc.identifier.issn0171-2985
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/2180
dc.description.abstractThis study explores the role of ovarian hormones in the phenotypic shaping of peripheral T-cell pool over the reproductive lifespan of rats. For this purpose, 2-month-old prepubertally ovariectomised (Ox) rats, showing oestrogen and progesterone deficiency, and 11-month-old Ox rats, exhibiting only progesterone deficiency, were examined for thymus output, and cellularity and composition of major TCR alpha beta+ peripheral blood lymphocyte (PBL) and splenocyte subsets. Although ovariectomy increased thymic output in both 2- and 11-month-old rats, the count of both CD4+ and CD8+ PBLs and splenocytes increased only in the former. In the blood and spleen of 11-month-old Ox rats only the count of CD8+ cells increased. Although ovariectomy affected the total CD4+ count in none of the examined compartments from the 11-month-old rats, it increased CD4+FoxP3+ PBL and splenocyte relative proportions over those in the age-matched controls. The age-related differences in the cellularity and the major subset composition in Ox rats were linked to the differences in the ovarian steroid hormone levels registered in 2- and 11-month-old rats. The administration of progesterone to Ox rats during the seven days before the sacrificing confirmed contribution of this hormone deficiency to the ovariectomy-induced changes in the TCR alpha beta+ PBL and splenocyte pool from 11-month-old rats. The expansion of the CD8+ splenocyte subset in the 11-month-old Ox rats reflected increases in cellularity of memory and, particularly, nave cells. This was due to greater thymic output of CD8+ cells and homeostatic proliferation than apoptosis in 11-month-old Ox rats when compared with age-matched sham-Ox control rats. The homeostatic changes within CD8+ splenocyte pool from 11-month-old Ox rats, most likely, reflected the enhanced splenic IL-7 and TGF-beta mRNA expression. Overall, in adult female rats, circulating oestrogen and progesterone provide maintenance of T-cell counts, a diversity of T-cell repertoire, and the main T-cell subset composition in the periphery. Progesterone deficiency affects mainly the CD8+ lymphocyte compartment through increasing thymic CD8+ cell export and upsetting homeostatic regulation within the CD8+ splenocyte pool. These alterations were reversible through progesterone supplementation.en
dc.publisherElsevier Gmbh, Urban & Fischer Verlag, Jena
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//
dc.rightsrestrictedAccess
dc.sourceImmunobiology
dc.subjectImmunoregulatory cytokinesen
dc.subjectPeripheral blood lymphocytesen
dc.subjectProgesteroneen
dc.subjectReproductive lifespanen
dc.subjectSplenocytesen
dc.subjectT-cell subsetsen
dc.titleReshaping of T-lymphocyte compartment in adult prepubertaly ovariectomised rats: A putative role for progesterone deficiencyen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПилиповић, Иван; Стојић-Вуканић, Зорица; Косец, Душко; Лепосавић, Гордана; Нацка-Aлексић, Мирјана; Ђикић, Јасрнина; Перишић-Нанут, Милица; Aрсеновић-Ранин, Невена;
dc.citation.volume219
dc.citation.issue2
dc.citation.spage118
dc.citation.epage130
dc.citation.other219(2): 118-130
dc.citation.rankM22
dc.identifier.wos000330555700006
dc.identifier.doi10.1016/j.imbio.2013.08.004
dc.identifier.pmid24054944
dc.identifier.scopus2-s2.0-84891827662
dc.identifier.rcubconv_3011
dc.type.versionpublishedVersion


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