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dc.creatorRadojević, Katarina
dc.creatorRakin, Ana
dc.creatorPilipović, Ivan
dc.creatorKosec, Duško
dc.creatorĐikić, Jasmina
dc.creatorBufan, Biljana
dc.creatorVujnović, Ivana
dc.creatorLeposavić, Gordana
dc.date.accessioned2019-09-02T11:41:04Z
dc.date.available2019-09-02T11:41:04Z
dc.date.issued2014
dc.identifier.issn0165-5728
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2182
dc.description.abstractThe present study, through quantification of tyrosine hydroxylase (TH) expression and catecholamine (CA) content in the presence and in the absence of alpha-methyl-p-tyrosine (AMPT), a TH inhibitor, in adult thymic organ (ATOC) and thymocyte culture, demonstrated that thymic cells produce CAs. In addition, in ATOC an increase in beta(2)-adrenoceptor (AR) mRNA expression and beta(2)-AR thymocyte surface density was registered. Furthermore, AMPT (10(-4) M), as propranolol (10(-4) M), augmented thymocyte apoptosis and diminished thymocyte proliferation in ATOC. Propranolol exerted these effects acting on CD3(high) thymocytes. However, in thymocyte cultures, propranolol (10(-6) M) acting on the same-thymocyte subset exerted the opposing effect on thymocyte apoptosis and ConA-stimulated proliferation. This suggested that, depending on thymocyte microenvironment, differential effects can be induced through the same type of AR. Additionally, arterenol (10(-8) to 10(-6) M), similar to propranolol, diminished apoptosis, but increased ConA-stimulated thymocyte proliferation in thymocyte culture. However, differently from propranolol, arterenol affected manly CD3- thymocyte subset, which harbors majority of alpha(1)-AR+ thymocytes. Additionally, arterenol showed a dose-dependent decrease in efficiency of thymocyte apoptosis and proliferation modulation with the rise in its concentration. Considering greater affinity of arterenol for alpha(1)-ARs than for beta(2)-ARs, the previous findings could be attributable to increased engagement of beta(2)-ARs with the rise of arterenol concentration. Consistently, in the presence of propranolol (10(-6) M), a beta-AR blacker, the arterenol (10(-8) M) effects on thymocytes were augmented. In conclusion, thymic endogenous CAs, acting through distinct AR types and, possible, the same AR type (but in different cell microenvironment) may exert the opposing effects on thymocyte apoptosis/proliferation.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Neuroimmunology
dc.subjectAdult thymus organ cultureen
dc.subjectThymocyte cultureen
dc.subjectPropranololen
dc.subjectArterenolen
dc.subjectThymocyte apoptosisen
dc.subjectThymocyte proliferationen
dc.titleEffects of catecholamines on thymocyte apoptosis and proliferation depend on thymocyte microenvironmenten
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПилиповић, Иван; Лепосавић, Гордана; Вујновић, Ивана; Буфан, Биљана; Радојевић, Катарина; Ракин, Aна; Косец, Душко; Ђикић, Јасмина;
dc.citation.volume272
dc.citation.issue1-2
dc.citation.spage16
dc.citation.epage28
dc.citation.other272(1-2): 16-28
dc.citation.rankM23
dc.identifier.wos000338613500003
dc.identifier.doi10.1016/j.jneuroim.2014.04.010
dc.identifier.pmid24837703
dc.identifier.scopus2-s2.0-84902002547
dc.identifier.rcubconv_3121
dc.type.versionpublishedVersion


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