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Multifunctional PLGA particles containing poly(L-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity
dc.creator | Stevanović, Magdalena | |
dc.creator | Bracko, Ines | |
dc.creator | Milenković, Marina | |
dc.creator | Filipović, Nenad | |
dc.creator | Nunić, Jana | |
dc.creator | Filipić, Metka | |
dc.creator | Uskoković, Dragan P. | |
dc.date.accessioned | 2019-09-02T11:41:09Z | |
dc.date.available | 2019-09-02T11:41:09Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1742-7061 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/2184 | |
dc.description.abstract | A water-soluble antioxidant (ascorbic acid, vitamin C) was encapsulated together with poly(L-glutamic acid)-capped silver nanoparticles (AgNpPGA) within a poly(lactide-co-glycolide) (PLGA) polymeric matrix and their synergistic effects were studied. The PLGA/AgNpPGA/ascorbic acid particles synthesized by a physicochemical method with solvent/non-solvent systems are spherical, have a mean diameter of 775 nm and a narrow size distribution with a polydispersity index of 0.158. The encapsulation efficiency of AgNpPGA/ascorbic acid within PLGA was determined to be >90%. The entire amount of encapsulated ascorbic acid was released in 68 days, and the entire amount of AgNpPGAs was released in 87 days of degradation. The influence of PLGA/AgNpPGA/ascorbic acid on cell viability, generation of reactive oxygen species (ROS) in HepG2 cells, as well as antimicrobial activity against seven different pathogens was investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGA/ascorbic acid particles. We measured the kinetics of ROS formation in HepG2 cells by a DCFH-DA assay, and found that PLGA/AgNpPGA/ascorbic acid caused a significant decrease in DCF fluorescence intensity, which was 2-fold lower than that in control cells after a 5 h exposure. This indicates that the PLGA/AgNpPGA/ascorbic acid microspheres either act as scavengers of intracellular ROS and/or reduce their formation. Also, the results of antimicrobial activity of PLGA/AgNpPGA/ascorbic acid obtained by the broth microdilution method showed superior and extended activity of these particles. The samples were characterized using Fourier transform infrared spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, zeta potential and particle size analysis. This paper presents a new approach to the treatment of infection that at the same time offers a very pronounced antioxidant effect. | en |
dc.publisher | Elsevier Sci Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS// | |
dc.rights | restrictedAccess | |
dc.source | Acta Biomaterialia | |
dc.subject | Antioxidant | en |
dc.subject | Silver nanoparticles | en |
dc.subject | PLGA | en |
dc.subject | Biomedical applications | en |
dc.subject | Antimicrobial activity | en |
dc.title | Multifunctional PLGA particles containing poly(L-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Филипић, Метка; Филиповић, Ненад; Ускоковић, Драган П.; Миленковић, Марина; Брацко, Инес; Стевановић, Магдалена; Нунић, Јана; | |
dc.citation.volume | 10 | |
dc.citation.issue | 1 | |
dc.citation.spage | 151 | |
dc.citation.epage | 162 | |
dc.citation.other | 10(1): 151-162 | |
dc.citation.rank | aM21 | |
dc.identifier.wos | 000329893300017 | |
dc.identifier.doi | 10.1016/j.actbio.2013.08.030 | |
dc.identifier.pmid | 23988864 | |
dc.identifier.scopus | 2-s2.0-84888641756 | |
dc.type.version | publishedVersion |